IEE Proceedings - Nanobiotechnology
Volume 153, Issue 3, June 2006
Volume 153, Issue 3
June 2006
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- Author(s): J. Kim ; G.L. Liu ; Y. Lu ; L.P. Lee
- Source: IEE Proceedings - Nanobiotechnology, Volume 153, Issue 3, p. 42 –46
- DOI: 10.1049/ip-nbt:20050016
- Type: Article
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The utilisation of plasmonic effects in metallic nanostructures is gaining importance for applications in molecular sensing. Of special interest is the local field enhancement effect, which enables surface-enhanced Raman scattering and significantly boosts the sensitivity of the Raman technique. For in vivo biological research, the ability to excite the resonance of localised surface plasmon-polaritons within the biological window is often desired. A new nanostructure called the nano-crescent is introduced and exhibits strong plasmonic activities within the biological window using a novel intra-particle plasmonic coupling scheme. - Author(s): W. Bücking and T. Nann
- Source: IEE Proceedings - Nanobiotechnology, Volume 153, Issue 3, p. 47 –53
- DOI: 10.1049/ip-nbt:20050043
- Type: Article
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Efforts were made to realise a two-dimensional, on-line-coupled isotachophoresis-capillary zone electrophoresis system. The electrophoretic behaviour of gold nanoparticles was investigated with the idea that they could be used to improve the control of this electrophoretic set-up. The well-known citrate-ligated gold nanoparticles were not suitable for this application, because the ligand was desorbed, and the nanoparticle solutions were degraded. Therefore mercaptocarboxylic acids were used, because the chemisorption of thiols on the gold surface was improved. Isotachophoretic measurements were carried out with these nanoparticles. A size-dependent electrophoretic mobility was found according to theoretical predictions, and the surface and ζ-potential were discussed for the small particle range. A new method for concentration measurements of nanoparticles is presented by means of isotachophoresis. - Author(s): K.-K. Sin ; C.P.-Y. Chan ; W.-M. Leung ; M. Seydack ; R. Renneberg
- Source: IEE Proceedings - Nanobiotechnology, Volume 153, Issue 3, p. 54 –58
- DOI: 10.1049/ip-nbt:20050048
- Type: Article
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A solid-phase sandwich fluorescence immunoassay using nanocrystals of a fluorogenic precursor, fluorescein diacetate (FDA), conjugated with monoclonal antibodies for the detection of C-reactive protein (CRP), is described. FDA nanocrystals were coated with distearoylglycerophosphoethanolamine (DSPE), modified with amino(poly(ethylene glycol))(PEG(2000)-Amine) as an interface for coupling biomolecules. CRP was chosen as a model analyte because of its widely accepted role as a marker for acute inflammation and prospective heart failure. A low limit of detection (1.10 μg l−1) and high precision (CV=2.72–9.48%) were achieved. Following the immunoreaction, the monoclonal anti-CRP conjugated nanocrystals were released by hydrolysis and dissolution instigated by the addition of a large volume of organic solvent–sodium hydroxide mixture. Using human serum samples from 66 patients with high heart attack risk and 19 healthy blood donors, this CRP fluorescence immunoassay showed a good correlation to the commercially available, turbidimetric immunoassay for CRP. This result was corroborated by the Bland–Altman plot that showed a mean difference between the two methods of only 0.36±1.46 mg l−1. The study demonstrates that the organic fluorogenic FDA nanocrystals can be applied for the detection of CRP, which is a clinically interesting plasma protein with a low limit of detection.
Spectral tuning of localised surface plasmon-polariton resonance in metallic nano-crescents
Electrophoretic analysis of gold nanoparticles: size-dependent electrophoretic mobility of nanoparticles
Fluorogenic nanocrystals for highly sensitive detection of C-reactive protein
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