IET Nanobiotechnology
Volume 13, Issue 2, April 2019
Volumes & issues:
Volume 13, Issue 2
April 2019
-
- Author(s): Zeinab Tohidiyan ; Shahla Hashemi ; Kaveh Parvanak Boroujeni
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 101 –106
- DOI: 10.1049/iet-nbt.2018.5003
- Type: Article
- + Show details - Hide details
-
p.
101
–106
(6)
NiO nanoparticles in high purity, 15 ± 0.5 nm in size, were prepared via solid-state microwave irradiation. The [Ni(NH3)6](NO3)2 complex as a novel source was decomposed in the presence of microwave irradiation for a short time (10 min). The present method is facile, safe, and low-cost. This method exhibits other advantages; there is no need of a solvent, fuel, surfactant, expensive material, or complex instrument. Synthesised NiO nanoparticles were determined by various analyses. Also, for the first time, NiO nanoparticle effects on biochemical factors in soybean were investigated. Seeds of soybean were grown in the Murashige and Skoog agar medium containing different concentrations of NiO nanoparticles (0, 200, and 400 mg/L) for 21 days under growth chamber conditions. Estimates of malondialdehyde, hydrogen peroxide contents, and antioxidant enzymes (catalase and ascorbate peroxidase) under treatment of NiO nanoparticles were assayed. The result showed that by significantly increasing the concentration of NiO nanoparticles, the activity of catalase and ascorbate peroxidase enzymes was enhanced. Malondialdehyde and hydrogen peroxide contents significantly increased in the presence of NiO nanoparticles. In this study, the increasing activity of catalase and ascorbate peroxidase was not enough for radical oxygen species detoxification.
- Author(s): Vidhu Sara Vargis ; Chandhana Jayachandran Priya ; Harsha Surendran ; Suneesh Punathil Vasu ; Bipin Nair ; Satheesh Babu Thekkedath Gopalakrishnan
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 107 –113
- DOI: 10.1049/iet-nbt.2018.5150
- Type: Article
- + Show details - Hide details
-
p.
107
–113
(7)
This study describes the development and testing of a simple and novel enzyme-free nanolabel for the detection and signal amplification in a sandwich immunoassay. Gold nanoparticles decorated reduced graphene oxide (rGOAu) was used as the nanolabel for the quantitative detection of human immunoglobulin G (HIgG). The rGOAu nanolabel was synthesised by one pot chemical reduction of graphene oxide and chloroauric acid using sodium borohydride. The pseudo-peroxidase behaviour of rGOAu makes the nanolabel unique from other existing labels. The immunosensing platform was fabricated using self-assembled monolayers of 11-mercaptoundecanoic acid (11-MUDA) on a gold disc electrode. The covalent immobilisation of antibody was achieved through the bonding of the carboxyl group of 11-MUDA and the amino group of the antibody using chemical linkers [1-ethyl-3-(3-dimethylaminopropyl)carbodiimide] and N-hydroxysuccinimide. The fabricated immunosensor exhibited a linear range that included HIgG concentrations of 62.5–500 ng ml−1. The sensor was also used for the testing of HIgG in the blood sample.
- Author(s): Mohsen Mohammadi ; Sabrieh Assadi Shahisaraee ; Atiyeh Tavajjohi ; Negin Pournoori ; Samad Muhammadnejad ; Shahla Roodbar Mohammadi ; Reza Poursalehi ; Hamid Delavari H
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 114 –119
- DOI: 10.1049/iet-nbt.2018.5146
- Type: Article
- + Show details - Hide details
-
p.
114
–119
(6)
Fluconazole (FLZ) application as a highly successful commercial antifungal azole agent to treat the fungal infections is limited due to emergence of FLZ-resistant candida. In this study, the potential of green synthesised silver nanoparticles (NPs) as an antifungal agent against Candida albicans fungal pathogen is investigated. The extract of ginger (Zingiber officinale) and thyme (Thymus vulgaris) plays as reducing agent, capping agent and antifungal agent. The UV–visible spectroscopy shows the peak of surface plasmon resonance of synthesised Ag NPs after a period of time. The synthesised Ag NPs are spherical, with average sizes of 12 and 18 nm based on ginger and thyme extract, respectively. Fourier transform infrared spectroscopy confirms the adsorption of the plant extract on the surface of the as-prepared Ag NPs. Based on the minimum inhibitory concentration (MIC) method against Candida albicans, the antifungal activity of as-prepared green synthesised Ag NPs shows higher inhibitory in comparison to FLZ. Finally, the Ag NPs synthesised via thyme extract shows no cytotoxicity with concentration below 3.5 ppm, which can be considered as an appropriate candidate instead of FLZ to treat the superficial fungal infections.
- Author(s): Vinod Kumar Nathan ; Parvathi Ammini ; Jasna Vijayan
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 120 –123
- DOI: 10.1049/iet-nbt.2018.5230
- Type: Article
- + Show details - Hide details
-
p.
120
–123
(4)
Biosynthesis of nanoparticles through plant extracts is gaining attention due to the toxic free synthesis process. The environmental engineering applications of many metal oxide nanoparticles have been reported. In this study, iron oxide nanoparticles (Fe2O3-Nps) were synthesised using a simple biosynthetic method using a leaf extract of a mangrove plant Rhizophora mucronata through reduction of 0.01 M ferric chloride. Fe2O3-Np synthesis was revealed by a greenish colour formation with a surface plasmon band observed close to 368 nm. The stable Fe2O3-Np possessed excitation and emission wavelength of 368.0 and 370.5 nm, respectively. The Fourier-transform infrared spectral analysis revealed the changes in functional groups during formation of Fe2O3-Np. Agglomerations of nanoparticles were observed during scanning electron microscopic analysis and energy-dispersive X-ray spectroscopic analysis confirmed the ferric oxide nature. The average particle size of Fe2O3-Np based on dynamic light scattering was 65 nm. Based on transmission electron microscopic analysis, particles were spherical in shape and the crystalline size was confirmed by selected area electron diffraction pattern analysis. The synthesised Fe2O3-Np exhibited a good photodegradation efficiency with a reduction of 83 and 95% of phenol red and crystal violet under irradiation of sunlight and florescent light, respectively. This report is a facile synthesis method for Fe2O3-Np with high photodegradation efficiency.
- Author(s): S. Mohammad Sajadi ; Kamal Kolo ; Samir M. Hamad ; Sarbast A. Mahmud ; Mohammad Pirouei ; Keyvan Amjadian ; Karzan M. Khalid
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 124 –133
- DOI: 10.1049/iet-nbt.2018.5014
- Type: Article
- + Show details - Hide details
-
p.
124
–133
(10)
For the first time, through a fast, eco-friendly and economic method, the aqueous extract of the leaf of Euphorbia corollate was used to the green synthesis of the highly stable CuO@Magnetite@Hen Bone nanocomposites (NCs) as a potent antioxidant and antibacterial agent against Pseudomonas aureus, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae pathogenic bacteria. The biosynthesised NCs were identified using the scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy, elemental mapping, X-ray diffraction (XRD), Fourier transforms infrared spectroscopy and UV–vis analytical techniques. Also, the radical scavenging activity using (2,2-diphenyl-1-picrylhydrazyl) method was used to evaluate the antioxidant activity of the NCs. The stability of nanocatalyst was monitored using the XRD and SEM analyses after 30 days from its synthesis. Furthermore, its excellent catalytic activity, recycling stability, and high substrate applicability were demonstrated to the adsorption of the polycyclic aromatic hydrocarbons of the light crude oil from Shiwashok oil fields and destruction of methylene blue and methyl orange as harmful organic dyes at ambient temperature using UV–vis spectroscopy. Moreover, the green CuO@Magnetite@Hen Bone NCs were recovered and reused several times without considerable loss of its catalytic activity.
- Author(s): Jayapriya Maruthai ; Arulmozhi Muthukumarasamy ; Balraj Baskaran
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 134 –143
- DOI: 10.1049/iet-nbt.2018.5136
- Type: Article
- + Show details - Hide details
-
p.
134
–143
(10)
This work explores the rapid synthesis of silver nanoparticles (AgNPs) from Musa paradisiaca (M. paradisiaca) bract extract. The bio-reduction of Ag+ ion was recorded using ultraviolet–visible spectroscopy by a surface plasmon resonance extinction peak with an absorbance at 420 nm. The phytoconstituents responsible for the reduction of AgNPs was probed using Fourier transform infrared spectroscopy. The X-ray diffraction pattern confirmed the formation of crystalline AgNPs that were analogous to selected area electron diffraction patterns. Morphological studies showed that the obtained AgNPs were monodispersed with an average size of 15 nm. The biologically synthesised AgNPs showed higher obstruction against tested phytopathogens. The synthesised AgNPs exhibited higher inhibitory zone against fungal pathogen Alternaria alternata and bacterial pathogen Pseudomonas syringae. Free radical scavenging potential of AgNPs was investigated using 1,1-diphenyl-2-picryl hydroxyl and 2,2-azinobis (3-ethylbenzothiazoline)-6-sulphonic acid assays which revealed that the synthesised AgNPs act as a potent radical scavenger. The catalytic efficiency of the synthesised AgNPs was investigated for azo dyes, methyl orange (MO), methylene blue (MB) and reduction of o-nitrophenol to o-aminophenol. The results portrayed that AgNPs act as an effective nanocatalyst to degrade MO to hydrazine derivatives, MB to leucomethylene blue, and o-nitro phenol to o-amino phenol
- Author(s): Ayoub Nadi ; Damien Boyer ; Nicolas Charbonnel ; Aïcha Boukhriss ; Christiane Forestier ; Said Gmouh
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 144 –149
- DOI: 10.1049/iet-nbt.2018.5026
- Type: Article
- + Show details - Hide details
-
p.
144
–149
(6)
Azo dyes are widely used in industries and their release in the environment contributes to the pollution of effluents. The authors aim to develop a new eco-friendly water treatment method for the degradation of azo dyes based on in situ magnetic separation and immobilisation of bacterial cells. The immobilisation was achieved using superparamagnetic Fe3O4 nanoparticles and offers the possibility of reusing bacteria by magnetic separation for several degradation cycles. The iron–oxide nanoparticles were synthesised by reverse co-precipitation. The Gram-positive bacteria Bacillus subtilis were immobilised using iron–oxide nanoparticles by adsorption and then separated with an external magnetic field. Transmission electron microscopy observation showed that the particles' diameter was ∼20 nm with a narrow size distribution. Moreover, the iron–oxide nanoparticles were adsorbed onto the surface in order to coat the cells. B. subtilis has proved its ability to decolorise and degrade several azo dyes at different values of pH, with the highest decolorisation rate for Congo red. Furthermore, immobilised cells have a degradation activity similar to that of free cells. The system provided a degradation rate up to 80% and could be reused for seven batch cycles.
- Author(s): Khuram Shahzad Ahmad and Shaan Bibi Jaffri
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 150 –159
- DOI: 10.1049/iet-nbt.2018.5006
- Type: Article
- + Show details - Hide details
-
p.
150
–159
(10)
This investigation has for the first time utilised environmental resource Prunus cerasifera seed extract phytochemicals for the green synthesis of carpogenic ZnO nanoparticles (NPs). Spherical morphology and size range of 56.57–107.70 nm at variable calcination temperatures without the use of any external reducing agent was obtained. The synthesised NPs exhibited hexagonal wurtzite geometry with an average crystal size 5.62 nm and a band gap of 3.4 eV. Carpogenic NPs were investigated for optical, compositional, morphological, and phytochemical make up via ultraviolet spectroscopy (UV–Vis), Fourier transform infrared analysis, X-ray powder diffraction, scanning electron microscopy, and gas chromatography and mass spectrometry. Carpogenic NPs degraded methyl red up to 83% with pseudo-first-order degradation kinetics (R 2 = 0.88) in 18 min signifying their remediation role in environment in conformity with all principles of green chemistry. Photocatalytic assays were performed in direct solar irradiance. Nine pathogens of biomedical and agricultural significance having multi-drug resistance were inhibited in vitro via the Kirby–Bauer disc diffusion assay. The enhanced photocatalytic and antimicrobial inhibition not only makes carpogenic ZnO NPs a future photo-degradative candidate for environmental remediation but also a nanofertiliser, nanofungicide, and nanobactericide synthesised via bioinspired, biomimetic, green, and unprecedented route.
- Author(s): Maryam Akhbari ; Reza Hajiaghaee ; Reza Ghafarzadegan ; Sepideh Hamedi ; Mahdi Yaghoobi
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 160 –169
- DOI: 10.1049/iet-nbt.2018.5040
- Type: Article
- + Show details - Hide details
-
p.
160
–169
(10)
The potential of Mentha piperita in the iron nanoparticles (FeNPs) production was evaluated for the first time. The influences of the variables such as incubation time, temperature, and volume ratio of the extract to metal ions on the nanoparticle size were investigated using central composite design. The appearance of SPR bands at 284 nm in UV–Vis spectra of the mixtures verified the nanoparticle formation. Incubating the aqueous extract and metal precursor with 1.5 volume ratio at 50°C for 30 min leads to the formation of the smallest nanoparticles with the narrowest size distribution. At the optimal condition, the nanoparticles were found to be within the range of 35–50 nm. Experimental measurements of the average nanoparticle size were fitted well to the polynomial model satisfactory with R 2 of 0.9078. Among all model terms, the linear term of temperature, the quadratic terms of temperature, and mixing volume ratio have the significant effects on the nanoparticle average size. FeNPs produced at the optimal condition were characterised by transmission electron microscopy, thermogravimetry analysis (TGA), and Fourier-transform infrared spectroscopy. The observed weight loss in the TGA curve confirms the encapsulation of FeNPs by the biomolecules of the extract which were dissociated by heat.
- Author(s): Kalpana Vellore Nagarajan and Devi Rajeswari Vijayarangan
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 170 –177
- DOI: 10.1049/iet-nbt.2018.5011
- Type: Article
- + Show details - Hide details
-
p.
170
–177
(8)
Malaria is a dangerous disease affecting humans and animals in tropical and subtropical areas worldwide. According to recent estimates, 3.2 billion people are at risk of malaria. Many drugs are in practices to control this disease and their vectors. Eco-friendly control tools are needed to fight vectors of this important disease. Nanotechnology is playing a key role in the fight against many public health emergencies. In the present study, Lagenaria siceraria aqueous peel extract was used to prepare zinc oxide nanoparticles (ZnO NPs), then tested on Anopheles stephensi eggs, larvae and pupae. The L. siceraria-synthesised ZnO NPs were characterized additionally by FTIR, AFM, XRD, UV-Vis spectroscopy, EDX, and SEM spectroscopy The ovicidal, larvicidal, pupicidal and repellent activities of L. siceraria and green-synthesised ZnO NPs were analysed on A. stephensi. The potential mechanism of action of ZnO NPs was studied investigating the changes in various enzyme activities in A. stephensi IV instar larvae. Furthermore, the smoke toxicity of L. siceraria-based cones against A. stephensi evoked higher mortality if compared with the control. Overall, the present study concluded that L. siceraria peel extract and its mediated green synthesised ZnO NPs represent a valuable green option to manage against malaria vectors.
- Author(s): Zhu Chen ; Xu Ye ; Guo Qingkui ; Qian Wenliang ; Zhao Wen ; Wang Ning ; Zheng Min
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 178 –182
- DOI: 10.1049/iet-nbt.2018.5145
- Type: Article
- + Show details - Hide details
-
p.
178
–182
(5)
The present study is designed to analyse the antibacterial and anticancer effects of silver nanoparticles (AgNPs) synthesised from the Cymbopogon citratus, (lemongrass) (LG-AgNPs), which is widely used in ayurvedic drugs for treating various diseases. The LG-AgNPs were synthesised and characterised using ultraviolet (UV) spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis. The characterised LG-AgNPs was subjected to antimicrobial analysis by disc diffusion method against pathogenic bacteria and fungi. Furthermore, the cytotoxicity and anticancer activity of the LG-AgNPs were assessed with lung alveolar carcinoma cell line A549. Results depict that UV–visible spectra of LG-AgNPs showed strong absorption peak at 435 nm. The XRD study exposed LG-AgNPs crystals, which confirmed with TEM analysis exhibiting particle size ranging between 17 and 25.8 nm. The FTIR spectra recorded peaks at 3347, 2126, 1639, 659, 598 and 553 cm−1. The zone of inhibition study proves the LG-AgNPs possessed both antibacterial and antifungal activities. 3-(4, 5-dimethyl thiazoyl-2-yl)-(2,5-diphenyltetrazolium bromide) results show the cytotoxicity effect of LG-AgNPs in lung cancer cells. It also inhibited the cell migration and invasion at the dose of 25 µg ml−1 by increasing the apoptotic gene expression. The results reveal LG-AgNPs possess anticancer activities, proposing that it may be an alternative drug for allopathic drugs with lots of side effects used in lung cancer treatment.
- Author(s): Anahita Kharabi Masooleh ; Asadollah Ahmadikhah ; Abbas Saidi
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 183 –188
- DOI: 10.1049/iet-nbt.2018.5141
- Type: Article
- + Show details - Hide details
-
p.
183
–188
(6)
Recently the use of medicinal plants potential in the production of nanoparticles has received serious attention. Here, the main component of Camellia sinensis L. (green tea) extract was detected by spectroscopy and the optimal conditions were determined for their performance in green synthesis of silver nanoparticles at room temperature. Epigallocatechin gallate was identified as the dominant component in the extract as determined by spectroscopy, and it was established that its oxidation was a function of the solution pH. Transmission electron microscopy, dynamic light scattering, and visible absorption spectroscopy (UV-Vis) confirmed the reduction in silver ions to silver nanoparticles (Ag NPs). Controlling over Ag NPs shape and narrow size distribution was achieved with 10 ml green tea leaf extract solution and in different reaction pH. Spherical colloidal Ag NPs with well-defined hydrodynamic diameters (with average hydrodynamic size of 27.9–50.2 nm) were produced. Silver nitrate concentrations used in this study were lower than that of reported in similar works, and synthesis efficiency was also higher. Nanoparticles were perfectly spherical and their uniformity, compared to similar studies, was much higher. These NPs showed higher degree of stability and were aqueously stable for >10 months in dark glasses at 4°C.
- Author(s): Mohammad Ehsan Taghavizadeh Yazdi ; Jalil Khara ; Mohammad Reza Housaindokht ; Hamid Reza Sadeghnia ; Sedigheh Esmaeilzadeh Bahabadi ; Mohammad Sadegh Amiri ; Hasan Mosawee ; Danial Taherzadeh ; Majid Darroudi
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 189 –192
- DOI: 10.1049/iet-nbt.2018.5215
- Type: Article
- + Show details - Hide details
-
p.
189
–192
(4)
Silver nanoparticles (AgNPs) have been biosynthesised through the extracts of Ribes khorassanicum fruits, which served as the reducing agents and capping agents. Biosynthesised AgNPs have been found to be ultraviolet–visible (UV–vis) absorption spectra since they have displayed one surface plasmon resonance peak at 438 nm, attesting the formation of spherical NPs. These particles have been characterised by UV–vis, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy analysis. The formation of AgNPs at 1.0 mM concentration of AgNO3 has resulted in NPs that contained mean diameters in a range of 20–40 nm. The green-synthesised AgNPs have demonstrated high antibacterial effect against pathogenic bacteria (i.e. Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa). Biosynthesising metal NPs through plant extracts can serve as the facile and eco-friendly alternative for chemical and/or physical methods that are utilised for large-scale nanometal fabrication in various medical and industrial applications.
- Author(s): Parth Sarthi Nayak ; Stuti Pradhan ; Manoranjan Arakha ; Dileep Kumar ; Mohammed Saleem ; Bibekanand Mallick ; Suman Jha
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 193 –201
- DOI: 10.1049/iet-nbt.2018.5025
- Type: Article
- + Show details - Hide details
-
p.
193
–201
(9)
Nanoparticles fabricated using medicinal plant extract have great potential in the area of nanomedicine. High surface-to-volume ratio of nanoparticle enhances the local active biomolecules concentration, leading to many fold increase in the medicinal potentials. The silver nanoparticles (AgNPs) fabricated using indigenous medicinal plants of India, Azadirachta indica and Syzygium cumini, have shown a significant effect on the viability of prokaryotic and eukaryotic cells. Biofabrication of AgNP was confirmed using different spectroscopic and microscopic techniques. Extraction and purification of AgNP from non-conjugated plant moieties are done using centrifugation and size exclusion chromatography. The cytotoxic propensity of AgNP formulations was screened against Gram-positive (Bacillus subtilis), Gram-negative (Escherichia coli) bacteria, cancerous (HT1080) and non-cancerous (HEK293) cell lines. The nanoparticle formulations showed a relatively higher cytotoxic propensity against Gram-positive bacteria and cancerous cell lines. In addition, the surface roughness and reactive oxygen species (ROS) measurements indicated that AgNP formulations mediate the cell activity predominantly by ROS-mediated disruptive change in membrane morphology upon direct interaction with the membrane. Hence, the nanoparticle formulations show an enhanced selective cytotoxic propensity towards Gram-positive bacteria and cancerous cell lines.
- Author(s): Mohammad Fahiminia ; Narges Sadat Shamabadi ; Mahmoud Nasrollahzadeh ; S. Mohammad Sajadi
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 202 –213
- DOI: 10.1049/iet-nbt.2018.5175
- Type: Article
- + Show details - Hide details
-
p.
202
–213
(12)
For the first time, copper nanoparticles (Cu NPs) superficially deposited on reduced graphene oxide (rGO) using Euphorbia cheiradenia Boiss leaf aqueous media. A beneficial series of analytical methods was used to characterise E. cheiradenia Boiss leaf extract and involved nanostructures. The Cu/rGO nanocomposite (NC) obtained from the conversion of Cu2+ ions to Cu NPs and GO to rGO undergoes the plant extract and used as a heterogeneous and reusable nanocatalyst for the destruction of 4-nitrophenol, rhodamine B, methylene blue, methyl orange and congo red using sodium borohydride at ambient temperature. In addition, Cu/rGO NC has reusability for many times in the reduction reactions with no decreasing of its catalytic capability.
- Author(s): Mohammad Rasouli
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 214 –218
- DOI: 10.1049/iet-nbt.2018.5187
- Type: Article
- + Show details - Hide details
-
p.
214
–218
(5)
Selenium (Se) is a rare and essential element for the human body and other living organisms because of its role in the structure of several proteins and having anti-oxidant properties to reduce oxidative stress at cells. Some microorganisms can absorb Se oxyanions and convert them into zero-valent Se (Se0) in the nanoscale dimensions, which can be used for producing Se nanoparticles (SeNPs). In the present study, SeNPs were intracellularly biosynthesised by yeast Nematospora coryli, which is an inexpensive method and does not involve using materials hazardous for human and environment. The produced NPs were refined by a two-phase system and then characterised and identified by ultraviolet–visible, X-ray diffraction, X-ray fluorescence, transmission electron microscope, and Fourier transform infrared spectroscopy analyses. The structural analysis of biosynthesised SeNPs showed spherical-shaped NPs with size ranging from 50 to 250 nm. Also, extracted NPs were applied to explore their anti-candida and anti-oxidant activities. The results of this investigation confirm the biological properties of Se.
- Author(s): Kitherian Sahayaraj ; Sathyamourthy Rajesh ; Jesu Antony Martin Rathi ; Vivek Kumar
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 219 –225
- DOI: 10.1049/iet-nbt.2018.5007
- Type: Article
- + Show details - Hide details
-
p.
219
–225
(7)
Silver nanoparticles (Ag NPs) were synthesised using the crude ethyl acetate extracts of Ulva lactuca and evaluated their bioefficacy against two crop-damaging pathogens. The sets of lattice planes in the XRD spectrum for the Ag NPs were indexed to the 111, 200, 220 and 311 orientations and support the crystalline nature of the Ag NPs. The 3414 and 2968 cm−1 peaks were observed in crude algal thallus extract and they were characteristic of terpenoids. Further, a peak at 1389 cm−1 was observed as fatty acids. The marine macroalgae terpenoids and palmitic acid acted as reducing agent and stabiliser, respectively. The size (3 and 50 nm) and shape (spherical) of Ag NPs were recorded. The energy-dispersive X-ray spectroscopy analysis exemplified the presence of silver in its elemental nature. Moreover, U. lactuca Ag NPs were effective against two cotton phytopathogens namely Fusarium oxysporum f.sp. vasinfectum (FOV) and Xanthomonas campestris pv. malvacearum (XAM). The minimum inhibitory concentration was found to be 80.0 and 43.33 μg ml−1 against FOV and XAM, respectively. Results confirmed the anti-microbial activity of green nanoparticles against select pathogens and suggest their possible usage in developing antifungal agents for controlling destructive pathogens in a cotton agroecosystem.
- Author(s): Kuttalam Sambamoorthy Uma Suganya ; Kasivelu Govindaraju ; Chitoor Veena Vani ; Mariappan Premanathan ; Vijaya Kumar Ganesh Kumar
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 226 –229
- DOI: 10.1049/iet-nbt.2018.5139
- Type: Article
- + Show details - Hide details
-
p.
226
–229
(4)
Diabetes mellitus has been considered as a heterogeneous metabolic disorder characterised by complete or relative impairment in the production of insulin by pancreatic β-cells or insulin resistance. In the present study, propanoic acid, an active biocomponent isolated from Cassia auriculata is employed for the synthesis of propanoic acid functionalised gold nanoparticles (Pa@AuNPs) and its anti-diabetic activity has been demonstrated in vitro. In vitro cytotoxicity of synthesised Pa@AuNPs was performed in L6 myotubes. The mode of action of Pa@AuNPs exhibiting anti-diabetic potential was validated by glucose uptake assay in the presence of Genistein (insulin receptor tyrosine kinase inhibitor) and Wortmannin (Phosphatidyl inositide kinase inhibitor). Pa@AuNPs exhibited significant glucose uptake in L6 myotubes with maximum uptake at 50 ng/ml. Assays were performed to study the potential of Pa@AuNPs in the inhibition of protein-tyrosine phosphatase 1B, α-glucosidases, and α-amylase activity.
- Author(s): Muhammad Iqbal ; Naveed Iqbal Raja ; Zia-Ur-Rehman Mashwani ; Feroza Hamid Wattoo ; Mubashir Hussain ; Muhammad Ejaz ; Hafiza Saira
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 230 –236
- DOI: 10.1049/iet-nbt.2018.5041
- Type: Article
- + Show details - Hide details
-
p.
230
–236
(7)
The present study was designed to check the role of silver nanoparticles (AgNPs) on physiological, biochemical parameters and antioxidants of wheat (Triticum aestivum L.) under heat stress. Plant extract of Moringa oleifera was used for AgNPs synthesis followed by characterization through UV–Vis spectroscopy, SEM, XRD and Zeta analyser. Heat stress was applied in range of 35–40°C for 3 hrs/ day for 3 days to wheat plants at trifoliate stage. Heat stress decreased the RWC (13.2%), MSI (16.3%), chl a (5.2%), chl b (4.1%) and TCCs (9.9%). Wheat plants treated with AgNPs showed significant increase in RWC (12.2%), MSI (26.5%), chl a (10%), chl b (16.4%), TCCs (19%), TPC (2.4%), TFC (2.5%), TASC (2.5%), SOD (1.3%), POX (1.5%), CAT (1.8%), APX (1.2%) and GPX (1.4%), under heat stress. Lower concentration of AgNPs (50 mg/l) decreased the sugar (5.8%) and proline contents (4%), while increase was observed in higher AgNPs concentrations. Overall, AgNPs treatment enhanced thermo-tolerance in wheat plants, but the mechanism of AgNPs action needs further investigation at genome and proteome level in wheat plants under heat stress.
- Author(s): Debarati Dey ; Pradipta Roy ; Debashis De
- Source: IET Nanobiotechnology, Volume 13, Issue 2, p. 237 –241
- DOI: 10.1049/iet-nbt.2018.5227
- Type: Article
- + Show details - Hide details
-
p.
237
–241
(5)
The switching property of an optical single molecular switch based on a single DNA molecule guanine with a single walled carbon nanotube electrode has been investigated using density functional theory along with non-equilibrium Green's function based first principle approach. The semi-empirical model of this single bio-molecular switch has been operated at an ultra-high 25 THz frequency in mid-UV range. This single bio-molecule comprises switching activity upon UV photo-excitation. The influence of the highest occupied molecular orbital and lowest unoccupied molecular orbital gap and the quantum ballistic transmission into the switching activity are discussed in detail in this study. It has been observed that the maximum ON–OFF ratio, i.e. 327 is obtained at +0.8 V bias voltage. Theoretical results show that current through the twisted form is sufficiently larger than the straightened form, which recommends that this structure has smart prospective application in the future generation switching nanotechnology.
Facile microwave-assisted synthesis of NiO nanoparticles and its effect on soybean (Glycine max)
Gold nanoparticles decorated reduced graphene oxide nanolabel for voltammetric immunosensing
Green synthesis of silver nanoparticles using Zingiber officinale and Thymus vulgaris extracts: characterisation, cell cytotoxicity, and its antifungal activity against Candida albicans in comparison to fluconazole
Photocatalytic degradation of synthetic dyes using iron (III) oxide nanoparticles (Fe2O3-Nps) synthesised using Rhizophora mucronata Lam
Biosynthesis of reusable and recyclable CuO@Magnetite@Hen Bone NCs and its antioxidant and antibacterial activities: a highly stable magnetically nanocatalyst for excellent reduction of organic dyes and adsorption of polycyclic aromatic hydrocarbons
Fabrication and characterisation of silver nanoparticles using bract extract of Musa paradisiaca for its synergistic combating effect on phytopathogens, free radical scavenging activity, and catalytic efficiency
Immobilisation of bacteria onto magnetic nanoparticles for the decolorisation and degradation of azo dyes
Carpogenic ZnO nanoparticles: amplified nanophotocatalytic and antimicrobial action
Process optimisation for green synthesis of zero-valent iron nanoparticles using Mentha piperita
Lagenaria siceraria – synthesised ZnO NPs – a valuable green route to control the malaria vector Anopheles stephensi
Anticancer activity of green synthesised AgNPs from Cymbopogon citratus (LG) against lung carcinoma cell line A549
Green synthesis of stable silver nanoparticles by the main reduction component of green tea (Camellia sinensis L.)
Role of Ribes khorassanicum in the biosynthesis of AgNPs and their antibacterial properties
Silver nanoparticles fabricated using medicinal plant extracts show enhanced antimicrobial and selective cytotoxic propensities
Phytosynthesis of Cu/rGO using Euphorbia cheiradenia Boiss extract and study of its ability in the reduction of organic dyes and 4-nitrophenol in aqueous medium
Biosynthesis of Selenium Nanoparticles using yeast Nematospora coryli and examination of their anti-candida and anti-oxidant activities
Green preparation of seaweed-based silver nano-liquid for cotton pathogenic fungi management
In vitro biological evaluation of anti-diabetic activity of organic–inorganic hybrid gold nanoparticles
Assessment of AgNPs exposure on physiological and biochemical changes and antioxidative defence system in wheat (Triticum aestivum L) under heat stress
First principle study of the self-switching characteristics of the guanine based single optical molecular switch using carbon nanotube electrodes
Most viewed content
Most cited content for this Journal
-
Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells
- Author(s): Ramasamy Subbaiya ; Muthupandian Saravanan ; Andavar Raja Priya ; Konathala Ravi Shankar ; Masilamani Selvam ; Muhammad Ovais ; Ramachandran Balajee ; Hamed Barabadi
- Type: Article
-
Medicinal plants mediated the green synthesis of silver nanoparticles and their biomedical applications
- Author(s): Haajira Beevi Habeeb Rahuman ; Ranjithkumar Dhandapani ; Santhoshini Narayanan ; Velmurugan Palanivel ; Ragul Paramasivam ; Ramalakshmi Subbarayalu ; Sathiamoorthi Thangavelu ; Saravanan Muthupandian
- Type: Article
-
Copper/copper oxide nanoparticles synthesis using Stachys lavandulifolia and its antibacterial activity
- Author(s): Mehrdad Khatami ; Hossein Heli ; Peyman Mohammadzadeh Jahani ; Hakim Azizi ; Marcos Augusto Lima Nobre
- Type: Article
-
Green synthesis of silver nanoparticles for selective toxicity towards cancer cells
- Author(s): Kasivelu Govindaraju ; Karthikeyan Krishnamoorthy ; Suliman A. Alsagaby ; Ganesan Singaravelu ; Mariappan Premanathan
- Type: Article
-
Nanobiotechnology and its applications in drug delivery system: a review
- Author(s): Imran Khan ; Momin Khan ; Muhammad Naveed Umar ; Deog-Hwan Oh
- Type: Article