Titanium dioxide (TiO₂) has been vastly used commercially, especially as white pigment in paints, colorants, plastics, coatings, cosmetics. Certain industrial uses TiO₂ in diameter <100 nm. There are three common exposure routes for TiO₂: (i) inhalation exposure, (ii) exposure via gastrointestinal tract, (iii) dermal exposure. Inhalation and gastrointestinal exposure appear to be the most probable ways of exposure, although nanoparticle (NP) penetration is limited. However, the penetration rate may increase substantially when the tissue is impaired. When TiO₂ NPs migrate into the circulatory system, they can be distributed into all tissues including brain. In brain, TiO₂ lead to oxidative stress mediated by the microglia phagocytic cells which respond to TiO₂ NPs by the production and release of superoxide radicals that convert to multiple reactive oxygen species (ROS). The ROS production may also cause the damage of blood–brain barrier which then becomes more permeable for NPs. Moreover, several studies have showed neuron degradation and the impairment of spatial recognition memory and learning abilities in laboratory rodent exposed to TiO₂ NPs.

With the advancement in nanotechnology, nanoparticles are reported to have applications in various fields. Their positive role in the environment, especially in plant ecosystem, is extensively studied nowadays. Among the metal nanoparticles, the silver nanoparticles (AgNP) are receiving special attention because of their ability to increase the growth and yield in many crops. Although many studies are found containing toxic effects of AgNPs the perspective of the present review is to collect the information about their positive role in growth and yield enhancement of crops. During this brief overview, many important crop plants are found to have a positive response towards the application of AgNPs. The appropriate elucidation of physiological, biochemical and molecular mechanism of nanoparticles in plant leads to better plant growth and development. It is concluded from this review that cautious and sensible use of nanotechnology can warrant food security through boosting agricultural production.

The increasing use of nanoparticles and their occurrence in the environment has made it imperative to elucidate their impact on the environment. Although several studies have advanced the authors’ understanding of nanoparticle–plant interactions, their knowledge of the exposure of plants to nanoparticles and their effects on edible crop plants remain meager and is often paradoxical. The aim of this study was to increase their knowledge on the effect of zinc oxide (ZnO) nanoparticles on eggplant seed germination and seedling growth. ZnO nanoparticles had a negative effect on the growth of eggplant in plant tissue-culture conditions, as the growth of seedlings decreased with the increase in the concentration of ZnO nanoparticles. In contrast, ZnO nanoparticles enhanced eggplant growth under greenhouse conditions. The accumulation of ZnO nanoparticles in various parts of eggplant was observed through scanning electron microscopy of both plant tissue-culture and greenhouse-raised eggplant seedlings. To the best of their knowledge, this is the first study to report on ZnO nanoparticle accumulation in eggplant and its effect on seed germination and seedling growth.

Hydroxyapatite-shrimp crusts nanocomposite thin films were deposited on titanium substrates by electrophoretic technique, under different preparation conditions, for bone implant applications. Fourier transform infrared spectrometer, atomic force microscope, X-ray diffraction (XRD), optical microscope, and scanning electron microscope were employed to characterise the synthesised films. Vickers’ micro-hardness measurements revealed a value of 502 HV for the hydroxyapatite films and 314.55 HV for the nanocomposite films. XRD results confirmed the polycrystalline nature of the hydroxyapatite and hydroxyapatite-shrimp nanocomposite films. The in-vitro bioactivity test of the synthesised films in simulated body fluid showed very low dissolution rate. Antibacterial activity of synthesised films was investigated against E. coli bacteria.

In this study, the conversion of silver ions into ∼30.74 nm sized silver nanoparticles (AgNPs) was achieved in 30 min at a reaction temperature of 80–90°C in aqueous leaf extract of Artemisia afra. The synthesised AgNPs showed surface plasmon resonance in the range of 423–438 nm. Spherical and face-centred cubic nanoparticles were confirmed by transmission electron microscope (TEM) and X-ray diffraction (XRD) analysis, respectively. Fourier transform infra-red (FTIR) results indicated that the obtained nanoparticles were stabilised and capped through the carbonyl and carboxylate ion groups possibly from flavonoids, terpenoids, phenolics and esters content of the extracts. In addition, the AgNPs were assessed for their biological potentials against some microbes and, also, their free radical scavenging ability was established. The AgNPs exhibited interesting antimicrobial and antioxidant properties better than the aqueous extract of A. afra.

Research dealing with early diagnosis and efficient treatment in colon cancer to improve patient's survival is still under investigation. Chemotherapeutic agent result in high systemic toxicity due to their non-specific actions on DNA repair and/or cell replication. Traditional medicine such as Lycopodium clavatum (LC) has been claimed to have therapeutic potentials against cancer. The present study focuses on targeted drug delivery of cationic liposomal nanoformulated LC (CL-LC) in colon cancer cells (HCT15) and comparing the efficacy with an anti-colon cancer drug, 7-ethyl-10-hydroxy-camptothecin (SN38) along with its nanoformulated form (CL-SN38). The colloidal suspension of LC was made using thin film hydration method. The drugs were characterised using ultraviolet, dynamic light scattering, scanning electron microscopy, energy, dispersive X-ray spectroscopy. In vitro drug release showed kinetics of 49 and 89% of SN38 and LC, whereas CL-SN38 and CL-LC showed 73 and 74% of sustained drug release, respectively. Studies on morphological changes, cell viability, cytotoxicity, apoptosis, cancer-associated gene expression analysis of Bcl-2, Bax, p53 by real-time polymerase chain reaction and western blot analysis of Bad and p53 protein were performed. Nanoformulated LC significantly inhibited growth and increased the apoptosis of colon cancer cells indicating its potential anti-cancer activity against colon cancer cells.

Two different morphological forms of graphene nanosheets: improved reduced graphene oxide (IRGO) and modified reduced GO (rGO) (MRGO) have been synthesised by improved and modified methods, respectively. Physical characterisations of these graphene nanosheets were carried out using X-ray diffraction, Fourier transform infrared spectroscopy, and Raman spectroscopy. Colloidal stability of these nanosheets toward a selected bacterium (e.g. Staphylococcus aureus) was ascertained by zeta potential. In the present study, the authors for the first time made an attempt to study and compare the potentialities of these two different forms of graphene nanosheets as efficient bactericidal agents. Field-emission scanning electron microscopy and TEM with energy dispersive X-ray spectroscopy (EDAX) studies of IRGO and MRGO have been carried out to explore their underlying mechanism of antibacterial responses through physical as well as chemical interactions with the selected bacterial species.

Nanoparticles (NPs) are gaining special interest due to their recent applications as antimicrobial agents to defeat the massive threat of resistant pathogens. This study focused on the utilisation of Streptomyces isolate S12 purified from waste discharge soil in the biological synthesis of silver (Ag), selenium (Se), and zinc oxide (ZnO) NPs. The isolate S12 was related to Streptomyces enissocaesilis according to 16S rRNA sequence analysis, morphological characteristics, and biochemical reactions. The cell-free supernatant has been used for the synthesis of Ag, Se, and ZnO NPs. The synthesised NPs were characterised using ultraviolet–visible spectroscopy, dynamic light scattering (DLS), transmission electron microscopy, and Fourier transform infrared spectroscopy. The biogenic NPs were evaluated for antimicrobial effects against different Gram-positive and Gram-negative resistant isolates using the broth microdilution method. They showed antibacterial effect against standard and resistant isolates; Bacillus cereus, Staphylococcus aureus ATCC 29213, S. aureus S1.1, methicillin resistant S. aureus (MRSA 303, 402 and 807), Escherichia coli ATCC 12435, E. coli E7, Klebsiella pneumoniae ATCC 51503, K. pneumoniae K5, K112, Pseudomonas aeruginosa PAO1, and P. aeruginosa P8. This study showed the green synthesis of various NPs using Streptomyces isolate S12 which demonstrated diverse activities against multi-drug resistant isolates.

Facile green synthesis of silver nanoparticles (AgNPs) using an aqueous extract of Carissa carandas (C. carandas) leaves was studied. Fabrication of AgNPs was confirmed by the UV–visible spectroscopy which gives absorption maxima at 420 nm. C. carandas leaves are the rich source of the bioactive molecules, acts as a reducing and stabilising agent in AgNPs, confirmed by Fourier transforms infrared spectroscopy. The field emission scanning electron microscope revealed the spherical shape of biosynthesised AgNPs. A distinctive peak of silver at 3 keV was determined by energy dispersive X-ray spectroscopy. X-ray diffraction showed the facecentred cubic structure of biosynthesised AgNPs and thermal stability was confirmed by the thermogravimetric analysis. Total flavonoid and total phenolic contents were evaluated in biosynthesised AgNPs. Biosynthesised AgNPs showed free radical scavenging activities against 2, 2-diphenyl-1-picrylhydrazyl test and ferric reducing antioxidant power assay. In vitro cytotoxicity against hepatic cell lines (HUH-7) and renal cell lines (HEK-293) were also assessed. Finally, biosynthesised AgNPs were scrutinised for their antibacterial activity against methicillin-resistant Staphylococcus aureus, Shigella sonnei, Shigella boydii and Salmonella typhimurium. This study demonstrated the biofabrication of AgNPs by using C. carandas leaves extract and a potential in vitro biological application as antioxidant, anticancer and antibacterial agents.

Chondroitin sulphate is a sulphated glycosaminoglycan biopolymer composed over 100 individual sugars. Chondroitin sulphate nanoparticles (NPs) loaded with catechin were prepared by an ionic gelation method using AlCl₃ and optimised for polymer and cross-linking agent concentration, curing time and stirring speed. Zeta potential, particle size, loading efficiency, and release efficiency over 24 h (RE₂₄%) were evaluated. The surface morphology of NPs was investigated by scanning electron microscopy and their thermal behaviour by differential scanning calorimetric. Antioxidant effect of NPs was determined by chelating activity of iron ions. The cell viability of mesenchymal stem cells was determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide assay and the calcification of osteoblasts was studied by Alizarin red staining. The optimised NPs showed particle size of 176 nm, zeta potential of −20.8 mV, loading efficiency of 93.3% and RE₂₄% of 80.6%. The chatechin loaded chondroitin sulphate NPs showed 70-fold more antioxidant activity, 3-fold proliferation effect and higher calcium precipitation in osteoblasts than free catechin.

Silver nanoparticles (AgNPs) have attracted the attention of researchers due to their properties. Biological synthesis of AgNPs is eco-friendly and cost-effective preferred to physical and chemical methods, which utilize environmentally harmful agents and large amounts of energy. Microorganisms have been explored as potential biofactories to synthesize AgNPs. Bacterial NP synthesis is affected by Ag salt concentration, pH, temperature and bacterial species. In this study, Bacillus spp., isolated from soil, were screened for AgNP synthesis at pH 12 with 5 mM Ag nitrate (AgNO₃) final concentration at room temperature. The isolate with fastest color change and the best ultraviolet-visible spectrum in width and height were chosen as premier one. AgNO₃ and citrate salts were compared in terms of their influence on NP synthesis. Spherical Ag chloride (AgCl) NPs with a size range of 35–40 nm were synthesized in 1.5 mM Ag citrate solution. Fourier transform infrared analysis demonstrated that protein and carbohydrates were capping agents for NPs. In this study, antimicrobial and antitumor properties of the AgNP were investigated. The resulting AgCl NPs had bacteriostatic activity against four standard spp. And multi-drug resistant strain of Pseudomonas aeruginosa. These NPs are also cytotoxic to cancer cell lines MCF-7, U87MG and T293.

The flow injection combined with tris-(bipyridyl) ruthenium (II)-cerium (IV) chemiluminescence (CL) method based on the sensitisation of silver nanoparticles (AgNPs) has been established for the quantitative analysis of gatifloxacin (GFX). AgNPs were synthesised using the reaction between ammonia gas and silver nitrate solution and was used to increase the CL intensity of the proposed system. The enhancement of CL intensity was linear with the concentration of GFX added. Effects of different experimental parameters were studied. Under the optimal conditions, the linear relationship was established between the concentration range of 1.4 × 10⁻¹⁰–4.5 × 10⁻⁸ M, with the correlation coefficient (r²) 02E9999. The limit of detection was found to be 4.6 × 10⁻¹¹ M. The relative standard deviation obtained was 3.2% for six replicate measurements of GFX (1.2 × 10⁻⁹ M). The proposed CL method was applied to the commercial drug and the result was in agreement with the labelled amount. The technique was further adopted to the analysis of GFX in spiked urine samples. Negligible interference was found (variation in CL intensity <5%) from a few common foreign excipients applied in preparation of pharmaceutical drug. The comparative results with a few reported methods indicates that the proposed method is more sensitive than other methods..

In this study, the authors reported the first synthesis process of silver iodide (AgI) nanoparticles (NPs) by pulsed laser ablation of the AgI target in deionised distilled water. The optical and structural properties of AgI NPs were investigated by using UV–vis absorption, X-ray diffraction, scanning electron microscope (SEM), energy dispersive X-ray, Fourier transform infrared spectroscopy, and transmission electron microscope (TEM). The optical data showed the presence of plasmon peak at 434 nm and the optical bandgap was found to be 2.6 eV at room temperature. SEM results confirm the agglomeration and aggregation of synthesised AgI NPs. TEM investigation showed that AgI NPs have a spherical shape and the average particle size was around 20 nm. The particle size distribution was the Gaussian type. The results showed that the synthesised AgI NPs have antibacterial activities against both bacterial strains and the activities were more potent against gram-negative bacteria.

This study discusses the use of Co₃O₄ impregnated graphene (CoOIG) as an efficient adsorbent for the removal of methyl violet (MV) dye from wastewater. CoOIG nanocomposites have been prepared by pyrolyzing paraffin wax with cobalt acetate. The synthesised nanocomposite was characterised by X-ray diffraction, field emission scanning electron microscope, transmission electron microscope, Fourier transform infrared spectroscope, Raman spectroscopy, and Brunauer–Emmett–Teller isotherm studies. The above studies indicate that the composites have cobalt oxide nanoparticles of size 51–58 nm embedded in the graphene nanoparticles. The adsorption studies were conducted with various parameters, pH, temperature and initial dye concentration, adsorbent dosage and contact time by the batch method. The adsorption of MV dye by the adsorbent CoOIG was about 90% initially at 15 min and 98% dye removal at pH 5. The data were fitted in Langmuir, Freundlich, Temkin, and Dubinin–Radushkevich and Sips isotherm models. Various thermodynamic parameters like Gibbs free energy, enthalpy, and entropy of the on-going adsorption process have also been calculated.

Present investigation aimed to prepare, optimise, and characterise lipid nanocapsules (LNCs) for improving the solubility and bioavailability of efavirenz (EFV). EFV-loaded LNCs were prepared by the phase-inversion temperature method and the influence of various formulation variables was assessed using Box–Behnken design. The prepared formulations were characterised for particle size, polydispersity index (PdI), zeta potential, encapsulation efficiency (EE), and release efficiency (RE). The biocompatibility of optimised formulation on Caco-2 cells was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Then, it was subjected to ex-vivo permeation using rat intestine. EFV-loaded LNCs were found to be spherical shape in the range of 20–100 nm with EE of 82–97%. The best results obtained from LNCs prepared by 17.5% labrafac and 10% solutol HS15 when the volume ratio of the diluting aqueous phase to the initial emulsion was 3.5. The mean particle size, zeta potential, PdI, EE, drug loading%, and RE during 144 h of optimised formulation were confirmed to 60.71 nm, −35.93 mV, 0.09, 92.60, 7.39 and 55.96%, respectively. Optimised LNCs increased the ex vivo intestinal permeation of EFV when compared with drug suspension. Thus, LNCs could be promising for improved oral delivery of EFV.

This study reports the fabrication of cellulose nanoparticles through electrospraying the solution of cellulose in N,N-dimethylacetamide/lithium chloride solvent as well as investigating the effect of electrospraying conditions and molecular weight on the average size of electrosprayed nanoparticles. Electrospraying of cellulose was carried out with the following range for each factor, namely concentration = 1–3 wt%, voltage = 15–23 kV, nozzle–collector distance = 10–25 cm, and feed rate = 0.03–0.0875 ml/h. The smallest nanoparticles had an average size of around 40 nm. Results showed that lowering the solution concentration and feed rate, as well as increasing the nozzle–collector distance and applied voltage led to a decrease in the average size of the electrosprayed cellulose nanoparticles. Fourier transform infrared analysis proved that no chemical change had occurred in the cellulose structure after the electrospraying process. According to X-ray diffraction (XRD) results, cellulose nanoparticles showed a lower degree of crystallinity in comparison with the raw cellulose powder. XRD results also proved the absence of LiCl salt in the electrosprayed nanoparticles.

Small molecule-based amphiphiles self-assemble into nanostructures (micelles) in aqueous medium which are currently being explored as novel drug delivery systems. Here, naproxen-polyethylene glycol (N-PEG), a small molecule-derived amphiphile, has been synthesised, characterised and evaluated as hydrophobic drug carrier. ¹H, ¹³C Nuclear magnetic resonance (NMR), mass spectrometry (MS) and Fourier-transform infrared spectroscopy (FTIR) confirmed the formation of N-PEG and dynamic light scattering (DLS) revealed the formation of nano-sized structures of ∼228 nm. Transmission electron microscope (TEM) analysis showed aggregation behaviour of the structures with average size of ∼230 nm. Biodegradability aspect of the micellar-structured N-PEG was demonstrated by lipase-mediated degradation studies using DLS and TEM. High encapsulation efficiency followed by release in a sustained manner of a well-known anticancer drug, doxorubicin, demonstrated the feasibility of the new drug delivery system. These results advocate the promising potential of N-PEG micelles as efficient drug delivery system for specific delivery to cancerous cells in vitro and in vivo.

This study concerns the optimisation of green synthesis of manganese oxide nanoparticles (MnO NPs) with Dittrichia graveolens (L.) extract via response surface methodology (RSM). Central composite design was used to evaluate the effect of pH, time, and the extract to the metal ratio on the synthesised nanoparticles (NPs). Nine runs were designed to investigate the effect of each parameter while NPs were synthesised under different conditions. Considering the p-values (p-value < 0.05), it is indicated that the extract to the metal ratio was the most effective parameter. The synthesised NPs were characterised using UV–vis. Synthesis of the NPs by polyphenolic compounds of green reducing agent and their stabilisation by curcumin was confirmed by Fourier transform infrared spectra and the surface morphology of the spherical MnO NPs was studied by field-emission scanning electron microscopy and transmission electron microscope techniques. The present researchers claimed the optimal condition as follows: time = 56.7 min, pH = 7.2, and the extract to the metal ratio = 87.9 v/v. MnO NPs at optimum condition were then employed for degradation of industrial dyes and they showed high dye degradation activity against Rhodamine B and light green dye. The average size of the synthesised MnO NPs at optimal condition was claimed to be nearly 38 nm.

In the present study, silver nanoparticles (SNPs) were synthesised for the first time using Pseudomonas geniculata H10 as reducing and stabilising agents. The synthesis of SNPs was the maximum when the culture supernatant was treated with 2.5 mM AgNO₃ at pH 7 and 40°C for 10 h. The SNPs were characterised by field emission scanning electron microscopy-energy-dispersive spectroscopy, transmission electron microscopy, dynamic light scattering, X-ray diffraction and UV–vis spectroscopy. Fourier transform infrared spectroscopy indicated the presence of proteins, suggesting they may have been responsible for the reduction and acted as capping agents. The SNPs displayed 1,1-diphenyl-2-picrylhydrazyl (IC₅₀ = 28.301 μg/ml) and 2,2′-azinobis-3-ethylbenzothiazoline-6-sulphonate (IC₅₀ = 27.076 μg/ml) radical scavenging activities. The SNPs exhibited a broad antimicrobial spectrum against several human pathogenic Gram-positive and Gram-negative bacteria and Candida albicans. The antimicrobial action of SNPs was due to cell deformation resulting in cytoplasmic leakage and subsequent lysis. The authors’ results indicate P. geniculata H10 could be used to produce antimicrobial SNPs in a facile, non-toxic, cost-effective manner, and that these SNPs can be used as effective growth inhibitors in various microorganisms, making them applicable to various biomedical and environmental systems. As far as the authors are aware, this study is the first to describe the potential biomedical applications of SNPs synthesised using P. geniculata.

Silver (Ag) complexes of drugs and their nanosystems have great potential as antibacterials. Recently, an Ag complex of furosemide (Ag–FSE) has shown to be a promising antimicrobial. However, poor solubility of Ag–FSE could hamper its introduction into clinics. Therefore, the authors developed a nanosuspension of Ag–FSE (Ag–FSE_NS) for its solubility and antibacterial activity enhancement. The aim of this study was to introduce a novel nanoantibiotic with enhanced antibacterial efficacy. Ag–FSE_NS was prepared by precipitation–ultrasonication technique. Size, polydispersity index (PI) and zeta potential (ZP) of prepared Ag–FSE_NS were measured by dynamic light scattering, whereas surface morphology was determined using scanning electron microscopy (SEM). In vitro antibacterial activity was evaluated against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa using broth microdilution method. Size, PI and ZP of optimised Ag–FSE_NS1 were 191.2 ± 19.34 nm, 0.465 ± 0.059 and −55.7 ± 8.18 mV, respectively. SEM revealed that Ag–FSE_NS1 particles were rod or needle-like with smooth surfaces. Saturation solubility of Ag–FSE in NS increased eight-fold than pure Ag–FSE. Ag–FSE_NS1 exhibited two-fold and eight-fold enhancements in activity against E. coli and S. aureus, respectively. The results obtained showed that developed Ag–FSE_NS1 holds a promise as a topical antibacterial.

This study proposes a method for in situ local deoxyribonucleic acid (DNA) replication and detection in a long DNA strand through laser-induced heating and strong avidin–biotin binding. To achieve the target DNA replication, dielectrophoresis was generated to stretch and immobilise DNA strands on both ends of the electrode. Subsequently, local DNA sequences were replicated using thermal cycles generated by laser-induced heating. Replicated double-stranded DNA products were captured in situ on a solid surface and detected using the fluorescence intensity of quantum dots (Qdots). The results revealed that after six laser-induced thermal cycles, the replicated local DNA sequence could be detected by analysing the difference between Qdot fluorescent intensity before and after replication. The proposed method is expected to improve the efficiency of biosample gene sequence analysis.

Developing nanostructures for cancer treatment is growing significantly. Liposomal doxorubicin is a drug that is used in the clinic and represents a lot of benefits over doxorubicin. The development of multifunctional liposomes with different cancer treatment capability enables broader applications of doxorubicin chemotherapy. Many efforts were carried to prepare more effective liposomal formulation through loading gold nanoparticles (GNPs) in the formulation. Here, GNPs with an average size of 6 nm were loaded in liposomal formulation alongside doxorubicin. The hydrodynamic diameter of final formulation was 177.3 ± 33.9 nm that in comparison with liposomes without GNPs (112.5 ± 10.3 nm), GNPs-loaded liposomes showed the bigger hydrodynamic diameter. GNPs-loaded liposomes are slightly positively charged (4.4 ± 1.1 mV), while liposomes without loading the GNPs were negatively charged (−18.5 ± 1.6 mV). Doxorubicin was loaded in this formulation through active loading technique. Doxorubicin loading efficiency in gold-loaded liposomes is slightly lesser than liposomes without GNPs, but still considerably high in comparison to passive loading techniques.

The present work is emphasised on the bio-fabrication of silver and gold nanoparticles in a single step by a microwave-assisted method using the leaf extract of Synedrella nodiflora as both reducing and stabilising agent. The synthesised nanoparticles are highly stable and show surface plasmon resonance peak at 413 and 535 nm, respectively, for silver and gold nanoparticles in UV–Vis spectrum. The functional group responsible for the reduction of metal ions were obtained from Fourier transform infrared spectroscopy. The crystalline nature of nanoparticles with face-centred cubic geometry was confirmed by the X-ray diffraction and selected area electron diffraction patterns. The morphology and sizes of the silver and gold nanoparticles were obtained from transmission electron microscopy images. The nanoparticles exhibit effective antimicrobial activities against various pathogenic strains. These antimicrobial properties were analysed by employing agar well diffusion method. The nanoparticles show significant antioxidant properties, and it was determined using 2, 2-diphenyl-1-picrylhydrazyl assay. The nanoparticles also show potent catalytic activity in the degradation of anthropogenic pollutant dyes Congo red and eosin Y by excess NaBH₄. Thus, the current study demonstrates the potential use of S. nodiflora as a reducing and stabilising agent for the synthesis of silver and gold nanoparticles and their relevance in the field of biomedicine and catalysis.

Green synthesis of nanoparticles has fuelled the use of biomaterials to synthesise a variety of metallic nanoparticles. The current study investigates the use of xylanases of Aspergillus niger L3 (NEA) and Trichoderma longibrachiatum L2 (TEA) to synthesise silver nanoparticles (AgNPs). Characterisation of AgNPs was carried out using UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy, while their effectiveness as antimicrobial, antioxidant, catalytic, anticoagulant, and thrombolytic agents were determined. The colloidal AgNPs was brownish with surface plasmon resonance at 402.5 and 410 nm for NEA-AgNPs and TEA-AgNPs, respectively; while FTIR indicated that protein molecules were responsible for the capping and stabilisation of the nanoparticles. The spherical nanoparticles had size of 15.21–77.49 nm. The nanoparticles significantly inhibited the growth of tested bacteria (63.20–88.10%) and fungi (82.20–86.10%), and also scavenged DPPH (37.48–79.42%) and hydrogen peroxide (20.50–96.50%). In addition, the AgNPs degraded malachite green (78.97%) and methylene blue (25.30%). Furthermore, the AgNPs displayed excellent anticoagulant and thrombolytic activities using human blood. This study has demonstrated the potential of xylanases to synthesise AgNPs which is to the best of our knowledge the first record of such. The present study underscores the relevance of xylanases in nanobiotechnology.

Here, the authors describe a simple method to formulate the nanodispersion of hexaconazole (hexa); henceforth, referred to as nanohexaconazole (N-hexa) that is water soluble and effective against several species of Aspergillus. Size and shape of the prepared nanocomposite was determined with high-resolution transmission electron microscopy and field-emission scanning electron microscopy. Nanohexaconazole structure was further confirmed by Fourier-transform infrared spectroscopy and gas chromatography–mass spectrometry. The antifungal efficacy of nanohexaconazole (N-hexa) was studied in vitro, compared with micronised hexaconazole (M-hexa) at different doses (5 ppm, 10 ppm and control) against two food pathogenic fungi: Aspergillus niger (MTCC 282, MTCC 2196 and BDS 113) and Aspergillus fumigatus through poisoned food technique. A dose-dependent significant growth inhibition was observed in nanohexaconazole (N-hexa) treated fungal sample compared with that of micronised hexaconazole (M-hexa). Micrographic studies for the morphological analysis of control and nanohexaconazole (N-hexa) treated fungal samples were done, exhibited an alternation in fungal morphology. Results showed that nanohexaconazole (N-hexa) is more efficacious than commercially available micronised hexaconazole (M-hexa). In future nanohexaconazole (N-hexa) could be a possible candidate for modern medical science and also reduce damage to the environment from injudicious use of pesticides.