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access icon free Biosynthesis of silver nanoparticles using upland cress: purification, characterisation, and antimicrobial activity

© The Institution of Engineering and Technology
Received 29/08/2019, Accepted 11/11/2019, Revised 10/10/2019, Published 05/02/2020

Silver nanoparticles have traditionally been synthesised using physical and chemical methods, often requiring expensive equipment and reagents that pose risks to the environment. This work provides a green method for the biosynthesis of silver nanoparticles using leaf extracts from upland cress: Barbarea verna. Natural reducing agents within the leaf extracts of upland cress reduce silver ions from silver nitrates, resulting in the formation of silver nanoparticles. The silver nanoparticles were purified using centrifugation and extraction using Triton X-114. The resulting nanoparticles were characterised using UV–Vis spectroscopy, dynamic light scattering, atomic force microscopy, and scanning electron microscopy. Silver nanoparticles were shown to have a diameter of 30–40 nm with a characteristic UV–Vis absorption peak at 420 nm. Antimicrobial properties of the synthesised silver nanoparticles were also confirmed using S. epidermis and E. coli bacteria.

Inspec keywords: nanofabrication; microorganisms; nanoparticles; silver; visible spectra; ultraviolet spectra; nanomedicine; atomic force microscopy; antibacterial activity; scanning electron microscopy; biotechnology; light scattering

Other keywords: biosynthesis; UV–Vis absorption peak; wavelength 420.0 nm; Barbarea verna; Triton X-114; dynamic light scattering; atomic force microscopy; scanning electron microscopy; size 30.0 nm to 40.0 nm; silver nitrates; upland cress; UV–vis spectroscopy; antimicrobial activity; S. epidermis; Ag; E. coli bacteria; antimicrobial properties; silver nanoparticles; physical method; chemical method

Subjects: Biomedical materials; Visible and ultraviolet spectra of metals, semimetals, and alloys; Nanotechnology applications in biomedicine; Solid surface structure; Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials

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http://iet.metastore.ingenta.com/content/journals/10.1049/mnl.2019.0528
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