access icon free Ultrasensitive immunosensor for AFP detection based on Cu2O to generate electrical signals

In this experiment, a Cu2O spherical nanoparticle was reduced by hydrothermal reaction to prepare a label-free electrochemical immunosensor based on a novel signal amplification strategy. Multi-walled carbon nanotubes are a platform that combines cuprous oxide with carbon nanotubes. Nanogold was modified on the surface of cuprous oxide nanospheres by electrostatic adsorption to further amplify the electrochemical signal and link-supported anti-alpha-fetoprotein (AFP). Under optimal conditions, the proposed immunosensor has a wide linear range from 0.001 to 40 ng/ml, with a detection limit as low as 0.33 pg/ml (S/N = 3). Furthermore, the proposed label-free immunosensor has been used to determine AFP in human serum with satisfactory results. At the same time, it has good reproducibility, acceptable selectivity and long-term stability, and has broad application prospects in biometric analysis.

Inspec keywords: gold; molecular biophysics; adsorption; nanoparticles; biosensors; electrochemical sensors; nanosensors; biochemistry; proteins; semiconductor materials; nanomedicine; multi-wall carbon nanotubes; biomedical equipment; nanofabrication; electrochemistry

Other keywords: electrochemical signal; ultrasensitive immunosensor; antialpha-fetoprotein; multiwalled carbon nanotubes; signal amplification strategy; label-free electrochemical immunosensor; electrostatic adsorption; electrochemical activity; carboxylate; optimal conditions; label-free immunosensor; cuprous oxide nanospheres; AFP detection; spherical nanoparticles; detection limit; human serum; Au-C-Cu2O; nanogold; electrical signals; hydrothermal reaction

Subjects: Physical chemistry of biomolecular solutions and condensed states; Biosensors; Chemical sensors; Microsensors and nanosensors; Biosensors; Sorption and accommodation coefficients (surface chemistry); Electrochemical analytical methods; Patient diagnostic methods and instrumentation; Nanotechnology applications in biomedicine; Electrochemistry and electrophoresis; Biomedical measurement and imaging; Electromagnetic radiation spectrometry (chemical analysis); Chemical sensors

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http://iet.metastore.ingenta.com/content/journals/10.1049/mnl.2019.0267
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