Nanopore biosensors have attracted attention due to their label-free single molecule detection capability. To date, different materials and applications have been shown in the field, varying from Si₃N₄ to graphene and biomolecule sensing to DNA sequencing. Classical nanopore devices are composed of Si₃N₄ material supported on a Si wafer and the detection is largely based on electrochemical sensing using chambers of ml volumes on both sides of the nanopore device. In this study, in-flow label-free electrochemical detection of DNA molecules at single molecule level is shown using a classical Si₃N₄ nanopore device integrated into a microfluidic device. The layout of the device given here set the basics for future works and discussions regarding future microfluidic integrated solid-state nanopores and the behaviour of the molecule under the influence of hydrodynamic flow.

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Development of in-flow label-free single molecule sensors using planar solid-state nanopore integrated microfluidic devices

References

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