Development of in-flow label-free single molecule sensors using planar solid-state nanopore integrated microfluidic devices
- Author(s): Fatma D. Güzel 1, 2 and Benjamin Miles 2
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View affiliations
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Affiliations:
1:
Biomedical Engineering , Ankara Yildirim Beyazit University , 06010 Etlik/Ankara , Turkey ;
2: Department of Chemistry , Imperial College London , South Kensington Campus , London, SW7 2AZ , UK
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Affiliations:
1:
Biomedical Engineering , Ankara Yildirim Beyazit University , 06010 Etlik/Ankara , Turkey ;
- Source:
Volume 13, Issue 9,
September
2018,
p.
1352 – 1357
DOI: 10.1049/mnl.2018.5206 , Online ISSN 1750-0443
Nanopore biosensors have attracted attention due to their label-free single molecule detection capability. To date, different materials and applications have been shown in the field, varying from Si3N4 to graphene and biomolecule sensing to DNA sequencing. Classical nanopore devices are composed of Si3N4 material supported on a Si wafer and the detection is largely based on electrochemical sensing using chambers of ml volumes on both sides of the nanopore device. In this study, in-flow label-free electrochemical detection of DNA molecules at single molecule level is shown using a classical Si3N4 nanopore device integrated into a microfluidic device. The layout of the device given here set the basics for future works and discussions regarding future microfluidic integrated solid-state nanopores and the behaviour of the molecule under the influence of hydrodynamic flow.
Inspec keywords: electrochemical sensors; silicon compounds; flow sensors; microsensors; microfluidics; nanoporous materials; nanosensors; biological techniques; flow measurement; DNA; biosensors; elemental semiconductors; silicon
Other keywords: biomolecule sensing; graphene; label-free single molecule detection capability; hydrodynamic flow; planar solid-state nanopore integrated microfluidic devices; DNA molecule sequencing; solid-state nanopore biosensors; Si3N4-Si; in-flow label-free electrochemical detection; in-flow label-free single molecule sensors; electrochemical sensing
Subjects: Molecular biophysics; Chemical sensors; Level, flow and volume measurement; Microsensors and nanosensors; Electrochemical analytical methods; Biosensors; Biological engineering and techniques; Biosensors; MEMS and NEMS device technology; Measurement instrumentation and techniques for fluid dynamics; Chemical variables measurement; Microfluidics and nanofluidics; Chemical sensors; Micromechanical and nanomechanical devices and systems
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