Development of in-flow label-free single molecule sensors using planar solid-state nanopore integrated microfluidic devices

Fatma D. Güzel; Benjamin Miles

Development of in-flow label-free single molecule sensors using planar solid-state nanopore integrated microfluidic devices

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Author(s): Fatma D. Güzel^{1, 2} and Benjamin Miles²
- Affiliations: 1: Biomedical Engineering , Ankara Yildirim Beyazit University , 06010 Etlik/Ankara , Turkey ;
  2: Department of Chemistry , Imperial College London , South Kensington Campus , London, SW7 2AZ , UK
Source: Volume 13, Issue 9, September 2018, p. 1352 – 1357
DOI: 10.1049/mnl.2018.5206 , Online ISSN 1750-0443

Received 28/02/2018, Accepted 08/06/2018, Revised 22/05/2018, Published 01/09/2018

Nanopore biosensors have attracted attention due to their label-free single molecule detection capability. To date, different materials and applications have been shown in the field, varying from Si₃N₄ to graphene and biomolecule sensing to DNA sequencing. Classical nanopore devices are composed of Si₃N₄ material supported on a Si wafer and the detection is largely based on electrochemical sensing using chambers of ml volumes on both sides of the nanopore device. In this study, in-flow label-free electrochemical detection of DNA molecules at single molecule level is shown using a classical Si₃N₄ nanopore device integrated into a microfluidic device. The layout of the device given here set the basics for future works and discussions regarding future microfluidic integrated solid-state nanopores and the behaviour of the molecule under the influence of hydrodynamic flow.

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Development of in-flow label-free single molecule sensors using planar solid-state nanopore integrated microfluidic devices

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