Fast immunofluorescence lateral flow test strip approach for detection of homocysteine

Fast immunofluorescence lateral flow test strip approach for detection of homocysteine

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The study developed a novel quantitative detection method of homocysteine (Hcy), an independent risk factor for cardiovascular disease, based on an immunofluorescent test strip. The fluorescent nanospheres (F-NSs) were prepared by embedding fluorophores (Cy5) into poly(styrene-acrylate) copolymer nanospheres, then conjugated with streptavidin (SA) to obtain SA/F-NSs as a signal amplification label stored in sample diluents. Biotin–antibody was fixed on the conjugate pad to specifically capture S-adenosyl Hcy (SAH) in the sample, where SAH was transformed from S-adenosyl methionine (SAM) by Hcy S-methyltransferase catalysis using Hcy as a substrate, the formed SAH could be trapped by SA/F-NSs and biotin–antibody conjugates in a competitive way with SAH–bovine serum albumin fixed on the test line, a detection limit of 0.27 μM Hcy was achieved. The fluorescence intensity of F-NSs remained stable during 273 days of storage, the bioactivity of the test strip was stable during 12 months of storage, and the strip possessed good reproducibility (intra-assay variability of 5.8%). Furthermore, other structural analogues SAM and cysteine showed negative results, validating the excellent specificity of the strips.


    1. 1)
      • 1. Srivastav, K., Saxena, S., Mahdi, A.A., et al: ‘Increased serum level of homocysteine correlates with retinal nerve fiber layer thinning in diabetic retinopathy’, Mol. Vis., 2016, 22, pp. 13521360.
    2. 2)
    3. 3)
    4. 4)
    5. 5)
    6. 6)
    7. 7)
      • 7. Stanger, O., Herrmann, W., Pietrzik, K., et al: ‘DACH-LIGA homocystein (German, Austrian and Swiss Homocysteine Society): consensus paper on the rational clinical use of homocysteine, folic acid and B-vitamins in cardiovascular and thrombotic diseases: guidelines and recommendations’, Clin. Chem. Lab. Med., 2003, 41, (11), pp. 13921403.
    8. 8)
      • 8. Hankey, G.J., Eikelboom, J.W.: ‘Homocysteine and vascular disease’, Kluwer, 2000, 82, (6), pp. 726731.
    9. 9)
    10. 10)
    11. 11)
    12. 12)
    13. 13)
    14. 14)
    15. 15)
    16. 16)
    17. 17)

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