AC-electric-field-induced parthenogenesis of mouse oocyte

Hong-Yuan Huang; Wei-Lun Kao; Yi-Wen Wang; Da-Jeng Yao

AC-electric-field-induced parthenogenesis of mouse oocyte

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Author(s): Hong-Yuan Huang¹ ; Wei-Lun Kao² ; Yi-Wen Wang¹ ; Da-Jeng Yao^{2, 3}
- Affiliations: 1: Department of Obstetrics and Gynecology, Lin-Kou Medical Center , Chang Gung Memorial Hospital , Taoyuan , Taiwan ;
  2: Department of Power Mechanical Engineering , National Tsing Hua University , Hsinchu 30013 , Taiwan ;
  3: Institute of NanoEngineering and MicroSystems, National Tsing Hua University , Hsinchu 30013 , Taiwan
Source: Volume 13, Issue 6, June 2018, p. 794 – 797
DOI: 10.1049/mnl.2017.0354 , Online ISSN 1750-0443

Received 13/06/2017, Accepted 21/02/2018, Revised 17/10/2017, Published 01/06/2018

To prevent parthenogenesis effect under embryo formation from sperms and oocyte, this study demonstrates that parthenogenesis of the oocyte of an ICR mouse can be induced with an AC electric field and without sperm insemination. The mouse oocytes were trapped with a positive dielectrophoretic force at the electrodes on an ITO-glass chip. The rates of parthenogenesis were tested under conditions of varied intensity of electric field from 33.35 kV/m to 266.8 kV/m and trapping durations 1, 3, 5 and 10 min. The results indicated that there is no significant statistical difference with varied trapping time (p > 0.1); with AC p-DEP electric activation, there is a significant statistical difference for 133.6 kV/m and 266.8 kV/m of electric field (p = 0.0013 and 0.0001 vs. control group respectively). The rate of parthenogenesis of the control group, oocytes with no electric activation, was 10.17±0.17% (n = 205). The rates of parthenogenesis were 51.65±7.8% (n = 27) at electric field 133.6 kV/m and 79.9±4.85% (n = 35) at 266.8 kV/m. The viability of these oocytes treated with an electric field exceeded 80%; this method of electric activation is hence safe for a mouse oocyte.

References

1. 1)
  - 5. Versieren, K., Heindryckx, B., Lierman, S., et al: ‘Developmental competence of parthenogenetic mouse and human embryos after chemical or electrical activation’, Reproductive BioMedicine Online, 2010, 21, (6), pp. 769–775 (doi: 10.1016/j.rbmo.2010.07.001).
2. 2)
  - 9. Thomas, B.J.: ‘Electromechanics of particles’ (Cambridge University Press, Cambridge UK, 1995), pp. 34–81.
3. 3)
  - 2. Mayyam Eftekhar, S.J., Rahsepar, M., Aflatoonian, A.: ‘Effect of oocyte activation with calcium ionophore on ICSI outcomes in teratospermia: A randomized clinical trial’, Iranian J. Reproductive Med., 2013, 11, (11), pp. 875–882.
4. 4)
  - 13. Melin, J., Lee, A., Foygel, K., et al: ‘In vitro embryo culture in defined, sub-microliter volumes’, Developmental Dynamics, 2009, 238, (4), pp. 950–955 (doi: 10.1002/dvdy.21918).
5. 5)
  - 7. Siracusa, G., Whittingham, D.G., Molinaro, M., et al: ‘Parthenogenetic activation of mouse oocytes induced by inhibitors of protein synthesis’, J. Embryology Exp. Morphology, 1978, 43, pp. 157–166.
6. 6)
  - 10. Choi, W., Kim, J.-S., Lee, D.-H., et al: ‘Dielectrophoretic oocyte selection chip for in vitro fertilization’, Biomed. Microdevices, 2008, 10, (3), pp. 337–345 (doi: 10.1007/s10544-007-9141-8).
7. 7)
  - 12. Malekshah, A.K., Moghaddam, A.E.: ‘The effect of culture medium volume on in vitro development of mouse embryos’, Iranian J. Reproductive Med., 2005, 3, (2), pp. 79–82.
8. 8)
  - 1. Jow, L.S., Johnston, T., Bonthron, D.T.: ‘A human parthenogenetic chimaera’, Nature Genetics, 1995, 11, pp. 164–169 (doi: 10.1038/ng1095-164).
9. 9)
  - 4. Idris, S.K., Abdullah, R.B., Embong, W.K.W., et al: ‘Comparison between different combinations of chemical treatment on parthenogenetic activation of mouse oocytes and its subsequent embryonic development’, Animal Cells Syst., 2013, 17, (3), pp. 196–202 (doi: 10.1080/19768354.2013.807877).
10. 10)
  - 11. Grosse, C., Schwan, H.P.: ‘Cellular membrane potentials induced by alternating fields’, Biophys. J., 1992, 63, (6), pp. 1632–1642 (doi: 10.1016/S0006-3495(92)81740-X).
11. 11)
  - 8. Huang, H.-Y., Huang, Y.-H., Kao, W.-L., et al: ‘Embryo formation from low sperm concentration by using dielectrophoretic force’, Biomicrofluidics, 2015, 9, (2), (doi: 10.1063/1.4915612).
12. 12)
  - 6. Mishra, V., Misra, A.K., Sharma, R.: ‘A comparative study of parthenogenic activation and in vitro fertilization of bubaline oocytes’, Animal Reproduction Sci., 2008, 103, (3-4), pp. 249–259 (doi: 10.1016/j.anireprosci.2006.12.019).
13. 13)
  - 3. Ma, S.-F., Liu, X.-Y., Miao, D., et al: ‘Parthenogenetic activation of mouse oocytes by strontium chloride: a search for the best conditions’, Theriogenology, 2005, 64, (5), pp. 1142–1157 (doi: 10.1016/j.theriogenology.2005.03.002).

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