© The Institution of Engineering and Technology
Silica/polydopamine/silver (SiO2/PD/Ag) nanoparticles (NPs) with a core–shell–satellite structure were fabricated and the mechanisms of their antibacterial activity were investigated. In this reported work, the results of the reactive oxygen species (ROS) assays, the deoxyribonucleic acid (DNA) damage assays and a cell morphology observation confirmed that Vibrio natriegens, a gram negative bacterium and Bacillus subtilis, a gram positive bacterium could be inhibited by the NPs. Gram negative bacteria exhibited more sensitivity towards the Ag NPs because these NPs were associated with penetration into the cytoplasm, with the subsequent local interaction of Ag with the cell components; thus, causing damages to the cells. SiO2/PD/Ag NPs produced ROS which caused damage to the DNA leading to the suppression of transcription as detected by a reporter gene assay. Furthermore, ROS induced membrane damage was determined by transmission electron microscopy. Thus, the mechanisms of antibacterial activity were interpreted more precisely by using the aforementioned experiments. The results revealed that the production of ROS and damage to the membrane were the two major mechanisms of the bactericidal action of SiO2/PD/Ag NPs; thus, these NPs could be employed as effective antifouling agents.
References
-
-
1)
-
3. Xu, G., Qiao, X., Qiu, X.: ‘New method about preparation of single dispersion spheric nanoparticles and antibacterial property’, Rare Met. Mater. Eng., 2008, 37, (9), pp. 1669–1672.
-
2)
-
1. Jones, W.L., Sutton, M.P., McKittrick, L.: ‘Chemical and antimicrobial treatments change the viscoelastic properties of bacterial biofilms’, Biofouling, 2011, 27, (2), pp. 207–215 (doi: 10.1080/08927014.2011.554977).
-
3)
-
22. Kimura, K., Tran, L.S.P., Uchida, I., et al: ‘Characterization of Bacillus subtilis γ-glutamyltransferase and its involvement in the degradation of capsule poly-γ-glutamate’, Microbiology, 2004, 150, (12), pp. 4115–4123 (doi: 10.1099/mic.0.27467-0).
-
4)
-
6. Perelshtein, I., Applerot, G., Perkas, N., et al: ‘CuO–cotton nanocomposite: formation, morphology, and antibacterial activity’, Surf. Coat. Technol., 2009, 204, (1–2), pp. 54–57 (doi: 10.1016/j.surfcoat.2009.06.028).
-
5)
-
29. Adams, L.K., Lyon, D.Y., Alvarez, P.J.J.: ‘Comparative eco-toxicity of nanoscale TiO2, SiO2, and ZnO water suspensions’, Water Res., 2006, 40, (19), pp. 3527–3532 (doi: 10.1016/j.watres.2006.08.004).
-
6)
-
J.R. Morones ,
J.L. Elechiguerra ,
A. Camacho
.
The bactericidal effect of silver nanoparticles.
Nanotechnology
,
10 ,
2346 -
2353
-
7)
-
X. Chen ,
H.J. Schluesener
.
Nanosilver: a nanoproduct in medical application.
Toxicol. Lett.
,
1 ,
1 -
12
-
8)
-
27. You, J., Zhang, Y., Hu, Z.: ‘Bacteria and bacteriophage inactivation by silver and zinc oxide nanoparticles’, Colloids Surf. B, Biointerfaces, 2011, 85, (2), pp. 161–167 (doi: 10.1016/j.colsurfb.2011.02.023).
-
9)
-
23. Pramanika, A., Lahaa, D., Bhattacharyaa, D., et al: ‘A novel study of antibacterial activity of copper iodide nanoparticle mediated by DNA and membrane damage’, Colloids Surf. B, Biointerfaces, 2012, 96, (1), pp. 50–55 (doi: 10.1016/j.colsurfb.2012.03.021).
-
10)
-
26. Kawano, T.: ‘Prion-derived copper-binding peptide fragments catalyze the generation of superoxide anion in the presence of aromatic monoamines’, Int. J. Biol. Sci., 2007, 3, (1), pp. 57–63 (doi: 10.7150/ijbs.3.57).
-
11)
-
M. Rai ,
A. Yadav ,
A. Gade
.
Silver nanoparticles as a new generation of antimicrobials.
Biotechnol. Adv.
,
76 -
83
-
12)
-
15. Saha, S., Leung, K.C., Nguyen, T.D., et al: ‘Nanovalves’, Adv. Funct. Mater., 2007, 17, (5), pp. 685–693 (doi: 10.1002/adfm.200600989).
-
13)
-
7. Xu, X., Yang, Q., Wang, Y.: ‘Biodegradable electrospun poly(L-lactide)fibers containing antibacterial silver nanoparticles’, Eur. Polym. J., 2006, 42, (9), pp. 2081–2087 (doi: 10.1016/j.eurpolymj.2006.03.032).
-
14)
-
25. Yamanaka, M., Hara, K., Kudo, J.: ‘Bactericidal actions of a silver ion solution on Escherichia coli, studied by energy-filtering transmission electron microscopy and proteomic analysis’, Appl. Environ. Microb., 2005, 71, (11), pp. 7589–7593 (doi: 10.1128/AEM.71.11.7589-7593.2005).
-
15)
-
8. Yang, H., Lin, C., Huang, C.: ‘Application of nanosilver surface modification to RO membrane and spacer for mitigating biofouling in seawater desalination’, Water Res., 2009, 43, (15), pp. 3777–3786 (doi: 10.1016/j.watres.2009.06.002).
-
16)
-
30. Stoimenov, P.K., Klinger, R.L., Marchin, G.L., et al: ‘Metal oxide nanoparticles as bactericidal agents’, Langmuir, 2002, 18, (17), pp. 6679–6686 (doi: 10.1021/la0202374).
-
17)
-
21. Taglietti, A., Fernandez, Y.A.D., Amato, E., et al: ‘Antibacterial activity of glutathione-coated silver nanoparticles against gram positive and gram negative bacteria’, Langmuir, 2012, 28, (21), pp. 8140–8148 (doi: 10.1021/la3003838).
-
18)
-
Q. Li ,
S. Mehendra ,
D.Y. Lyon
.
Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications.
Water Res.
,
18 ,
4591 -
4602
-
19)
-
9. Siegrist, M., Stampfli, N., Kastenholz, H., et al: ‘Perceived risks and perceived benefits of different nanotechnology foods and nanotechnology food packaging’, Appetite, 2008, 51, (2), pp. 283–290 (doi: 10.1016/j.appet.2008.02.020).
-
20)
-
28. Zhang, L., Jiang, Y., Ding, Y., et al: ‘Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofluids)’, J. Nanopart. Res., 2007, 9, (3), pp. 479–489 (doi: 10.1007/s11051-006-9150-1).
-
21)
-
14. Chamakura, K., Perez-Ballestrero, R., Luo, Z., et al: ‘Comparison of bactericidal activities of silver nanoparticles with common chemical disinfectants’, Colloids Surf. B, Biointerfaces, 2011, 84, (1), pp. 88–96 (doi: 10.1016/j.colsurfb.2010.12.020).
-
22)
-
24. Hwang, E.T., Lee, J.H., Chae, Y.J., et al: ‘Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria’, Small, 2008, 4, (6), pp. 746–750 (doi: 10.1002/smll.200700954).
-
23)
-
2. Ytreberg, E., Karlsson, J., Eklund, B.: ‘Comparison of toxicity and release rates of Cu and Zn from anti-fouling paints leached in natural and artificial brackish seawater’, Sci. Total Environ., 2010, 408, (12), pp. 2459–2466 (doi: 10.1016/j.scitotenv.2010.02.036).
-
24)
-
Q. Cai ,
Z.-S. Luo ,
W.Q. Pang ,
Y.-W. Fan ,
X.-H. Chen ,
F.-Z. Cui
.
Dilute solution routes to various controllable morphologies of MCM-41 silica with a basic medium.
Chem. Mater.
,
258 -
263
-
25)
-
5. Stephan, T.D., Panittamat, K., Pranut, P.: ‘Layer-by-layer deposition of antimicrobial silver nanoparticles on textile fibers’, Physicochem. Eng. Aspects, 2006, 289, (1–3), pp. 105–109 (doi: 10.1016/j.colsurfa.2006.04.012).
-
26)
-
11. Kumar, A., Vemula, P.K., Ajayan, P.M., et al: ‘Silver-nanoparticle-embedded antimicrobial paints based on vegetable oil’, Nat. Mater., 2008, 7, (3), pp. 236–241 (doi: 10.1038/nmat2099).
-
27)
-
20. Becerra, M.C., Eraso, A.J., Albesa, I.: ‘Comparison of oxidative stress induced by ciprofloxacin and pyoverdin in bacteria and in leukocytes to evaluate toxicity’, Luminescence, 2003, 18, (6), pp. 334–340 (doi: 10.1002/bio.742).
-
28)
-
18. Mignot, T., Denis, B., Couture-Tosi, E., et al: ‘Distribution of S-layers on the surface of Bacillus cereus strains: phylogenetic origin and ecological pressure’, Environ. Microbiol., 2001, 3, (8), pp. 493–501 (doi: 10.1046/j.1462-2920.2001.00220.x).
-
29)
-
31. Brayner, R., Ferrariliou, R., Brivois, N., et al: ‘Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium’, Nano Lett., 2006, 6, (4), pp. 866–870 (doi: 10.1021/nl052326h).
-
30)
-
Z. Shi ,
K.G. Neoh ,
E.T. Kang ,
W. Wang
.
Antibacterial and mechanical properties of bone cement impregnated with chitosan nanoparticles.
Biomaterials
,
11 ,
2440 -
2449
-
31)
-
17. Liong, M., Lu, J., Kovochich, M., et al: ‘Multifunctional inorganic nanoparticles for imaging, targeting, and drug delivery’, ACS Nano, 2008, 2, (5), pp. 889–896 (doi: 10.1021/nn800072t).
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