Your browser does not support JavaScript!
http://iet.metastore.ingenta.com
1887

Effects of micropost spacing and stiffness on cell motility

Effects of micropost spacing and stiffness on cell motility

For access to this article, please select a purchase option:

Buy article PDF
$19.95
(plus tax if applicable)
Buy Knowledge Pack
10 articles for $120.00
(plus taxes if applicable)

IET members benefit from discounts to all IET publications and free access to E&T Magazine. If you are an IET member, log in to your account and the discounts will automatically be applied.

Learn more about IET membership 

Recommend Title Publication to library

You must fill out fields marked with: *

Librarian details
Name:*
Email:*
Your details
Name:*
Email:*
Department:*
Why are you recommending this title?
Select reason:
 
 
 
 
 
Micro & Nano Letters — Recommend this title to your library

Thank you

Your recommendation has been sent to your librarian.

Microfabrication processes enable the biophysical control of cellular environments at the micro- and nanoscale. The mechanical properties of arrayed microposts have been demonstrated to influence diverse cellular functions including cell motility, yet the cellular response to changes in micropost spacing remains unclear. In this work, a microfabricated post array with variable spacing and stiffness was constructed to investigate the effects of these biophysical factors on cell motility. Over a length of 675 µm, the spacing between arrayed microposts decreased from 6 to 2 µm in a single direction, corresponding to an average spacing decrease of 40 nm from post-to-post. Simultaneously, the radii of 7 µm-high microposts were decreased from 2 to 1 µm, resulting in a decrease in micropost stiffness from ∼50 to 5 nN/µm, respectively. Over the course of 18 h studies, bovine aortic endothelial cells (BAECs) seeded on the microfabricated post array migrated for an average of 9.6±7.3 µm in the direction of decreasing interpost spacing, opposite the direction of durotaxis. By the end of the studies, 61% of seeded BAECs exhibited displacement in the direction of decreasing interpost spacing. The experimental results suggest that the spacing between microposts can be a determinant factor of cell migration direction in the design of micro- and nanotopographic cellular platforms.

References

    1. 1)
    2. 2)
    3. 3)
    4. 4)
    5. 5)
    6. 6)
    7. 7)
    8. 8)
    9. 9)
    10. 10)
    11. 11)
    12. 12)
    13. 13)
    14. 14)
    15. 15)
    16. 16)
    17. 17)
    18. 18)
      • N.D. Gallant , A.J. Garcia . Cell adhesion strengthening and focal adhesion assembly on micropatterned substrates. Mol. Biol. Cell , 173A - 173A
    19. 19)
    20. 20)
http://iet.metastore.ingenta.com/content/journals/10.1049/mnl.2011.0020
Loading

Related content

content/journals/10.1049/mnl.2011.0020
pub_keyword,iet_inspecKeyword,pub_concept
6
6
Loading
This is a required field
Please enter a valid email address