access icon free Assessment of acaricidal activity of nanoscale ZnO encapsulated piperine formulation against Rhipicephalus microplus

© The Institution of Engineering and Technology
Received 09/06/2020, Accepted 04/08/2020, Revised 17/07/2020, Published 24/08/2020

This study aims to synthesise and evaluate the acaricidal activity of nanoscale zinc oxide piperine formulation (NZPF) against Rhipicephalus microplus ticks. NZPF was prepared by using zinc oxide nanoparticles (ZnONPs) and piperine by employing encapsulation technique; characterised by UV spectroscopy, Fourier transformed infrared analysis, X-ray diffraction, dynamic light scattering, zetapotential and scanning electron microscopy. Acaricidal activity of the NZPF on R. microplus was evaluated using larval packet test (LPT) and adult immersion test (AIT). LPT against R. microplus larvae showed an LC50 at 1 mg/l for NZPF followed by 2 and 3 mg/l for ZnONPs and piperine, respectively. AIT against R. microplus showed an LC50 at concentration of 3 mg/l for NZPF followed by 6 mg/l for ZnONPs and 7 mg/l for piperine. In both LPT and AIT, LC50 values of ZnONPs and NZPF were significantly lower compared to deltamethrin. NZPF showed significant ovulation inhibitory activity with lower IC50 and IC99 values compared to ZnONPs and piperine. NZPF has been proved to be the better alternative to routine chemical acaricides for control of tick infestation of cattle in the wake of acaricidal resistance, but safety issues need to be addressed before clinical application.

Inspec keywords: nanomedicine; X-ray diffraction; agricultural safety; Fourier transform infrared spectra; biotechnology; light scattering; electrokinetic effects; pest control; nanoparticles; zinc compounds; agricultural engineering; molecular biophysics; ultraviolet spectra; scanning electron microscopy; encapsulation

Other keywords: ZnO; acaricidal resistance; AIT; scanning electron microscopy; X-ray diffraction; adult immersion test; larval packet test; Fourier transformed infrared analysis; Rhipicephalus microplus ticks; ZnONP; zinc oxide nanoparticles; LPT; NZPF; zetapotential; dynamic light scattering; acaricidal activity; UV spectroscopy; nanoscale zinc oxide piperine formulation

Subjects: Biotechnology industry; Agriculture; Industrial processes; Health and safety aspects; Testing; Packaging

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