access icon free Green synthesis of AgCl/Ag3PO4 nanoparticle using cyanobacteria and assessment of its antibacterial, colorimetric detection of heavy metals and antioxidant properties

© The Institution of Engineering and Technology
Received 10/03/2020, Accepted 04/08/2020, Revised 28/07/2020, Published 24/08/2020

In this study, the extract of two strains of cyanobacteria was used for the synthesis of silver nanoparticles (NPs). UV–vis spectroscopy, X-ray diffraction, dynamic light scattering and field emission scanning electron microscopy (FESEM) analyses were carried out to characterise the NPs. The antioxidant activity and heavy metal detection properties were investigated; moreover, their minimum inhibitory concentration and minimum bactericidal concentration against the multi-drug resistant bacteria were determined. The most abundant materials in these extracts were carbohydrates, so the biosynthesis of NPs using exopolysaccharide (EPS) was also investigated. The surface plasmon resonance of NPs had a peak at 435 nm and EPS NPs at 350–450 nm. The NPs produced by Nostoc sp. IBRC-M5064 extract revealed the face-centred cubic (fcc) structure of AgCl, while NPs of N. pruniforme showed the fcc crystalline structure of Ag3PO4 and AgCl. The FESEM showed the spherical shape of these NPs. The AgCl/Ag3PO4 colloid, in comparison with AgCl, showed better antioxidant activity and antibacterial effect. The heavy metal detection analysis of NPs revealed that the NPs of both stains involved in Hg (NO3)2 detection.

Inspec keywords: microorganisms; X-ray diffraction; field emission scanning electron microscopy; ultraviolet spectra; colloids; nanosensors; visible spectra; chemical sensors; surface plasmon resonance; nanofabrication; drugs; antibacterial activity; light scattering; nanoparticles; silver compounds; silver; biochemistry

Other keywords: X-ray diffraction analysis; colorimetric detection; inhibitory concentration; FESEM; spherical shape; exopolysaccharide; Nostoc sp. IBRC-M5064 extract; antibacterial effect; AgCl-Ag3PO4; antibacterial detection; cyanobacteria; silver nanoparticle synthesis; antioxidant activity; multidrug resistant bacteria; Ag; heavy metal detection analysis; face-centred cubic crystalline structure; UV-visible spectroscopy analysis; dynamic light scattering; field emission scanning electron microscopy analysis; surface plasmon resonance; wavelength 350.0 nm to 450.0 nm

Subjects: Physical chemistry of biomolecular solutions and condensed states; Optical properties of other inorganic semiconductors and insulators (thin films, low-dimensional and nanoscale structures); Visible and ultraviolet spectra (condensed matter); Other methods of nanofabrication; Chemical sensors; Microsensors and nanosensors; Chemical sensors; Colloids; Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Collective excitations (surface states)

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