access icon free Functionalisation of Fe3O4 nanoparticles by 2-((pyrazol-4-yl) methylene) hydrazinecarbothioamide enhances the apoptosis of human breast cancer MCF-7 cells

Cancer is a major cause of death. Thus, the incidence and mortality rate of cancer is globally important. Regarding vast problems caused by chemotherapy drugs, efforts have progressed to find new anti-cancer drugs. Pyrazole derivatives are known as components with anti-cancer properties. In here, Fe3O4 nanoparticles were first functionalized with (3-chloropropyl) trimethoxysilane, then 2-((pyrazol-4-yl) methylene) hydrazinecarbothioamide (P) was anchored on the surface of magnetic nanoparticles (PL). The synthesized nano-compounds were characterized using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, Zeta potential, dynamic light scattering, and energy-dispersive x-ray spectrometry analyses. The cytotoxicity effect was evaluated using MTT assay, apoptosis test by Flow cytometry, cell cycle analysis, Caspase-3 activity assay and Hoechst staining on MCF-7 cell line. The high toxicity for tumor cells and low toxicity on normal cells (MCF10A) was considered as an important feature (selectivity index, 10.9). Based on results, the IC50 for P and PL compounds were 157.80 and 131.84 μM/ml respectively. Moreover, apoptosis inducing, nuclear fragmentation, Caspase 3 activity and induction of cell rest in sub-G1 and S phases, were also observed. The inhibitory effect of PL was significantly higher than P, which could be due to the high penetrability of Fe3O4 nanoparticles.

Inspec keywords: X-ray diffraction; magnetic particles; Fourier transform infrared spectra; biomedical materials; tumours; iron compounds; cancer; light scattering; toxicology; cellular biophysics; biochemistry; enzymes; electrokinetic effects; drugs; nanomagnetics; nanomedicine; nanofabrication; X-ray chemical analysis; scanning electron microscopy; molecular biophysics; nanoparticles

Other keywords: MCF-7 cell line; human breast cancer MCF-7 cells; caspase-3 activity assay; mortality rate; nuclear fragmentation; pyrazole derivatives; cell cycle analysis; dynamic light scattering; anticancer properties; heterocyclic components; MCF10A nontumourigenic cells; cytotoxicity effect; chemotherapy drugs; Fe3O4; energy-dispersive X-ray spectrometry; nanocompounds; scanning electron microscopy; (3-chloropropyl) trimethoxysilane; X-ray diffraction; MTT assay; 2-((pyrazol-4-yl) methylene) hydrazinecarbothioamide; Zeta potential; tumour cells; Hoechst staining; Fe3O4 magnetic nanoparticles; apoptosis test; cell rest induction

Subjects: Magnetic properties of nanostructures; Physical chemistry of biomolecular solutions and condensed states; Cellular biophysics; Fine-particle magnetic systems; Brillouin and Rayleigh scattering; other light scattering (condensed matter); Electrochemistry and electrophoresis; Infrared and Raman spectra in inorganic crystals; Other methods of nanofabrication; Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Patient care and treatment; Optical properties of other inorganic semiconductors and insulators (thin films, low-dimensional and nanoscale structures); Biomedical materials; Electromagnetic radiation spectrometry (chemical analysis); Nanotechnology applications in biomedicine; Low-dimensional structures: growth, structure and nonelectronic properties; Amorphous and nanostructured magnetic materials

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http://iet.metastore.ingenta.com/content/journals/10.1049/iet-nbt.2019.0199
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