access icon free Surface stress-induced membrane biosensor based on double-layer stable gold nanostructures for E. coli detection

© The Institution of Engineering and Technology
Received 02/03/2019, Accepted 26/07/2019, Revised 22/07/2019, Published 29/07/2019

The surface stress-based biosensor has been applied in fast and sensitive identification of Escherichia coli (E. coli)with significance for public health, food, and water safety. However, the stable sensitive element of flexible biosensor based on surface stress is still crucial and challengeable. Here, the authors reported surface stress-induced biosensors based on double-layer stable gold nanostructures (D-AuNS-SSMB) for E. coli O157:H7 detection. Bacterial detection demonstrates the high stability of the biosensor. The resistance change of biosensor is linear to the logarithmic value of the E. coli O157:H7 concentrations ranging from 103 to 107 CFU/mL with a limit of detection (LOD) of 43 CFU/mL. The captured signals of D-AuNS-SSMB comes from surface stress generated by antigen–antibody binding. In addition, the biosensor exhibits good stability, reproducibility and specificity in detection of E. coli O157:H7 as well. This study provides a new preparation method of stable sensitive element for the E. coli detection.

Inspec keywords: nanosensors; biosensors; stress measurement; nanostructured materials; membranes; gold; microorganisms

Other keywords: surface stress-induced membrane biosensor; E. coli O157:H7 concentration detection; antigen–antibody binding; double-layer stable gold nanostructures; Au; escherichia coli detection; bacterial detection; water safety; D-AuNS-SSMB; stability

Subjects: Micromechanical and nanomechanical devices and systems; MEMS and NEMS device technology; Biological transport; cellular and subcellular transmembrane physics; Microsensors and nanosensors; Sensing and detecting devices; Mechanical variables measurement; Measurement of mechanical variables; Biosensors; Biosensors; Biological engineering and techniques

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