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access icon free Synthesis and characterisation of iron oxide nanoparticles conjugated with epidermal growth factor receptor (EGFR) monoclonal antibody as MRI contrast agent for cancer detection

  • Author(s): Zeinab Salehnia 1, 2 ; Daryoush Shahbazi-Gahrouei 1 ; Abolfazl Akbarzadeh 3 ; Behzad Baradaran 2 ; Safar Farajnia 4 ; Mehran Naghibi 5
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  • Source: Volume 13, Issue 4, June 2019, p. 400 – 406
    DOI:  10.1049/iet-nbt.2018.5285 , Print ISSN 1751-8741, Online ISSN 1751-875X
© The Institution of Engineering and Technology
Received 04/09/2018, Accepted 19/01/2019, Revised 30/10/2018, Published 28/01/2019

The aim of this study is to synthesise superparamagnetic iron oxide nanoparticles conjugated with anti-epidermal growth factor receptor monoclonal antibody (ANTI-EGFR-SPION) and investigate its physicochemical characterisation and biocompatibility as a targeted magnetic resonance imaging (MRI) contrast agent for the EGFR-specific detection in EGFR expressing tumour cells. These particles employed biocompatible polymers, poly(D,L-lactide-co-glycolide) (PLGA) and polyethylene glycol aldehyde (PEG-aldehyde), to increase the half-life of particles in circulation and reduce their side effects. The Fe3O4-loaded PLGA-PEG-aldehyde nanoparticles were prepared by a modified water-in-oil-in-water double emulsion method. The EGFR antibody was conjugated to the surface of SPIONs using the aldehyde-amine reaction. Synthesised conjugates (nanoprobes) were characterised using Fourier transform infrared spectrophotometry, dynamic light scattering, transmission electron microscopy images, and vibrating-sample magnetometery, and the results showed that the conjugation was successful. The mean diameter of nanoprobes was about 25 nm. These nanoprobes exhibited excellent water-solubility, stability, and biocompatibility. Meanwhile, MR susceptibility test proved that synthesised nanoprobes can be managed for negative contrast enhancement. The results of this study suggested the potential use of these nanoprobes for non-invasive molecular MRI in EGFR detection in the future.

Inspec keywords: light scattering; transmission electron microscopy; molecular biophysics; Fourier transform spectra; solubility; polymers; nanomagnetics; emulsions; tumours; nanoparticles; nanomedicine; cancer; proteins; superparamagnetism; magnetic particles; cellular biophysics; iron compounds; spectrophotometry; nanofabrication; biomedical MRI

Other keywords: targeted magnetic resonance imaging contrast agent; synthesised nanoprobes; Fe3O4; superparamagnetic iron oxide nanoparticles; anti-epidermal growth factor receptor monoclonal antibody; EGFR antibody; novel targeting cancer detection; PLGA-PEG-aldehyde nanoparticles; modified water-in-oil-in-water double emulsion method; physicochemical characterisation; size 25.0 nm; transmission electron microscopy images; biocompatible polymers; aldehyde-amine reaction; EGFR detection; ANTI-EGFR-SPION; EGFR-specific detection; synthesised conjugates were characterised using Fourier; biocompatibility; EGFR expressing tumour cells

Subjects: Patient diagnostic methods and instrumentation; Methods of nanofabrication and processing; Nanotechnology applications in biomedicine; Biomedical magnetic resonance imaging and spectroscopy; Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Medical magnetic resonance imaging and spectroscopy

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