access icon free Novel nanoplex-mediated plant transformation approach

© The Institution of Engineering and Technology
Received 04/09/2018, Accepted 17/05/2019, Revised 26/02/2019, Published 22/05/2019

Here, a rapid and easy transformation by electroporation technique for gene transfer in plants using cell penetrating amino nanocomplex (nanoplex) has been demonstrated in Nicotiana. Nanoplex was prepared using cell penetrating amino acids (CPAs) such as poly-L-lysine (PLL) and Argenine (Arg), in combination with the gold nanoparticles (AuNPs). PLLs-modified nanoplex with zeta potential of 34.2 ± 1.22 mV charge showed 63.3% efficiency for gene transformation in plant cells as compared to 60% when modified with Arg and the zeta potential was found to be 30.0 ± 0.83 mV; whereas, the transformation efficiency without nanoplex was found to be 6.6%. The findings indicate that the zeta potential of positively charged nanocomplex (AuNPs/CPAs/DNA/CPAs) increases the transformation efficiency because of their ability to protect the DNA from electroporation wave and endogenous enzyme damage. Transformation was confirmed by GUS assay and amplification of npt gene. This technique may open up new possibilities of gene transfer in plants, which will enable to produce large number of transgenic plants.

Inspec keywords: molecular biophysics; genomics; cellular biophysics; enzymes; DNA; biomedical materials; nanomedicine; genetics; gold; nanoparticles; biochemistry; electrokinetic effects

Other keywords: zeta potential; gene transfer; plant cells; AuNPs-CPAs-DNA-CPAs; electroporation wave; cell penetrating amino nanocomplex; voltage 29.169999999999998 mV to 30.830000000000002 mV; Au; transgenic plants; npt gene; gene transformation; nanoplex-mediated plant transformation approach; poly-L-lysine; cell penetrating amino acids; Arg; electroporation technique; transformation efficiency; gold nanoparticles; PLLs-modified nanoplex; positively charged nanocomplex; voltage 32.980000000000004 mV to 35.42 mV

Subjects: Physical chemistry of biomolecular solutions and condensed states; Nanotechnology applications in biomedicine; Genomic techniques; Biomolecular structure, configuration, conformation, and active sites; Biomedical materials; Macromolecular configuration (bonds, dimensions); Physics of subcellular structures; Electrochemistry and electrophoresis; Interactions with radiations at the biomolecular level

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