access icon free Efficacy of mycosynthesised AgNPs from Earliella scabrosa as an in vitro antibacterial and wound healing agent

© The Institution of Engineering and Technology
Received 31/07/2018, Accepted 28/11/2018, Revised 17/11/2018, Published 12/02/2019

The silver nanoparticles (AgNPs) with their unique chemical and physical properties are proving as a new therapeutical agent. In the present study, the AgNPs synthesised from an aqueous extract of a macrofungus, Earliella scabrosa, were characterised by field emission scanning electron microscopy (FESEM), energy dispersive X-ray analysis (EDX), high-resolution transmission electron microscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and further evaluate for its in vitro antibacterial and wound healing efficacy. The mycosynthesised AgNPs exhibited the surface plasmon resonance peak at 410 nm with good stability over a period of a month. The FESEM and EDX analyses revealed the spherical-shaped AgNPs of an average size of 20 nm and the presence of elemental Ag, respectively. The XRD pattern showed the crystalline nature of AgNPs. The FTIR spectra confirmed the conversion of Ag+ ions to AgNPs due to reduction by biomolecules of macrofungus extract. The mycosynthesised AgNPs showed effective antibacterial activity against two Gram-positive bacteria, namely Bacillus subtilis and Staphylococcus aureus, and two Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. The pathogens were highly sensitive to AgNPs, whereas less sensitive to AgNO3. The mycosynthesised AgNPs showed significant wound healing potential with 68.58% of wound closure.

Inspec keywords: wounds; field emission scanning electron microscopy; microorganisms; molecular biophysics; nanomedicine; reduction (chemical); biomedical materials; X-ray diffraction; nanoparticles; X-ray chemical analysis; transmission electron microscopy; surface plasmon resonance; antibacterial activity; silver; particle size; nanofabrication; Fourier transform infrared spectra

Other keywords: wound healing agent; spherical-shaped AgNPs; in vitro antibacterial efficacy; aqueous extract; EDX; Bacillus subtilis; FTIR spectroscopy; chemical properties; mycosynthesised AgNPs; FESEM; macrofungus; energy dispersive X-ray analysis; silver nanoparticles; crystalline nature; Escherichia coli; Ag; Gram-negative bacteria; Staphylococcus aureus; surface plasmon resonance peak; Pseudomonas aeruginosa; field emission scanning electron microscopy; physical properties; biomolecules; healing efficacy; XRD; pathogens; X-ray diffraction; Fourier transform infrared spectroscopy; Earliella scabrosa; therapeutical agent; Gram-positive bacteria; wound closure; high-resolution transmission electron microscopy

Subjects: Biomedical materials; Patient care and treatment; Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Collective excitations (surface states); Other methods of nanofabrication; Nanotechnology applications in biomedicine; Low-dimensional structures: growth, structure and nonelectronic properties; Electromagnetic radiation spectrometry (chemical analysis); Infrared and Raman spectra in metals; Optical properties of metals and metallic alloys (thin films, low-dimensional and nanoscale structures)

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