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access icon free Electrochemical assessment of the interaction of microbial living cells and carbon nanomaterials

© The Institution of Engineering and Technology
Received 21/06/2018, Accepted 24/12/2018, Revised 22/11/2018, Published 24/12/2018

This work considers the effects of various carbon nanomaterials and fibres on bioelectrocatalytic and respiratory activity of bacterial cells during the oxidation of ethanol in the presence of an electron transport mediator. Gluconobacter oxydans sbsp. industrius VKM B-1280 cells were immobilised on the surfaces of graphite electrodes and had an adsorption contact with a nanomaterial (multi-walled carbon nanotubes, thermally expanded graphite, highly oriented pyrolytic graphite, graphene oxide, reduced graphene oxide). The electrochemical parameters of the electrodes (the polarisation curves, the value of generated current at the introduction of substrate, the impedance characteristics) were measured in two-electrode configuration. Modification by multi-walled carbon nanotubes led to the increase of microbial fuel cell (MFC) electric power by 26%. The charge transfer resistance of modified electrodes was 47% lower than unmodified ones. Thermally expanded and pyrolytic graphites had a slight negative effect on the electrochemical properties of modified electrodes. The respiratory activity of bacterial cells did not change in the presence of nanomaterials. The data can be used in the development of microbial biosensors and MFC electrodes based on Gluconobacter cells.

Inspec keywords: catalysis; graphene; electrochemical electrodes; nanofabrication; multi-wall carbon nanotubes; adsorption; charge exchange; graphite; cellular biophysics; oxidation; microorganisms; microbial fuel cells

Other keywords: bioelectrocatalytic activity; electrochemical parameters; C; microbial biosensors; polarisation curves; microbial fuel cell; microbial living cells; thermally expanded graphite; bacterial cells; graphite electrodes; reduced graphene oxide; electron transport mediator; multiwalled carbon nanotubes; highly oriented pyrolytic graphite; MFC electrodes; adsorption contact; two-electrode configuration; Gluconobacter oxydans sbsp. industrius VKM B-1280 cells; charge transfer resistance; ethanol; respiratory activity

Subjects: Heterogeneous catalysis at surfaces and other surface reactions; Fuel cells; Sorption and accommodation coefficients (surface chemistry); Fuel cells; Cellular biophysics; Adsorption and desorption kinetics; evaporation and condensation; Electrochemistry and electrophoresis; Electrochemical analytical methods

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