access icon free Gold nanoparticles decorated reduced graphene oxide nanolabel for voltammetric immunosensing

© The Institution of Engineering and Technology
Received 01/06/2018, Accepted 14/08/2018, Published 07/09/2018

This study describes the development and testing of a simple and novel enzyme-free nanolabel for the detection and signal amplification in a sandwich immunoassay. Gold nanoparticles decorated reduced graphene oxide (rGOAu) was used as the nanolabel for the quantitative detection of human immunoglobulin G (HIgG). The rGOAu nanolabel was synthesised by one pot chemical reduction of graphene oxide and chloroauric acid using sodium borohydride. The pseudo-peroxidase behaviour of rGOAu makes the nanolabel unique from other existing labels. The immunosensing platform was fabricated using self-assembled monolayers of 11-mercaptoundecanoic acid (11-MUDA) on a gold disc electrode. The covalent immobilisation of antibody was achieved through the bonding of the carboxyl group of 11-MUDA and the amino group of the antibody using chemical linkers [1-ethyl-3-(3-dimethylaminopropyl)carbodiimide] and N-hydroxysuccinimide. The fabricated immunosensor exhibited a linear range that included HIgG concentrations of 62.5–500 ng ml−1. The sensor was also used for the testing of HIgG in the blood sample.

Inspec keywords: biochemistry; self-assembly; electrochemical sensors; nanofabrication; monolayers; biosensors; nanomedicine; blood; gold; nanoparticles; nanosensors; graphene; proteins; voltammetry (chemical analysis); reduction (chemical); molecular biophysics; chemical sensors; oxidation

Other keywords: N-hydroxysuccinimide; enzyme-free nanolabel; chloroauric acid; quantitative HIgG detection; pseudo-peroxidase behaviour; HIgG concentrations; gold nanoparticles; Au-CO; carboxyl group bonding; voltammetric immunosensing; blood sample; signal amplification; 11-mercaptoundecanoic acid; gold disc electrode; chemical linkers; reduced graphene oxide nanolabel; rGOAu nanolabel; sandwich immunoassay; immunosensor; human immunoglobulin G; sodium borohydride; 11-MUDA; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide]; self-assembled monolayers; covalent antibody immobilisation; one pot chemical reduction

Subjects: Chemical sensors; Methods of nanofabrication and processing; Nanotechnology applications in biomedicine; Electrochemistry and electrophoresis; Microsensors and nanosensors; Electrochemical analytical methods; Physical chemistry of biomolecular solutions and condensed states; Biosensors; Chemical sensors; Biosensors; Biomolecular interactions, charge transfer complexes; Biomedical measurement and imaging

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