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access icon free Silver nanoparticles and silver salt (AgNO3) elicits morphogenic and biochemical variations in callus cultures of sugarcane

© The Institution of Engineering and Technology
Received 15/05/2018, Accepted 19/06/2019, Revised 24/05/2019, Published 21/06/2019

The research work was arranged to check the role of AgNPs and silver ions on callus cells of sugarcane (Saccharum spp. cv CP-77,400). AgNPs were synthesized chemically and characterized by UV-Vis spectra, XRD and SEM. AgNPs and silver ions were applied in various concentrations (0, 20, 40, 60 ppm) to sugarcane calli and the induced stress was characterized by studying various morphological and biochemical parameters. AgNPs and silver ions treatments produced high levels of malondialdehyde, proline, proteins, TP and TF contents. Similarly, CAT, SOD and POX activity was also significant in both treatments. The lower concentration of AgNPs and silver ions (20 ppm) provided maximum intracellular GSH level. This work mainly showed effects of AgNPs and silver ions on sugarcane calli in terms of morphological aberrations and cell membrane damage due to severe oxidative stress and production of enhanced levels of enzymatic and non-enzymatic antioxidants as self-defence to tolerate oxidative stress by scavenging reactive oxygen species. These preliminary findings will provide the way to study ecotoxicity mechanism of the metal ions and NPs in medicine industry and in vitro toxicity research. Furthermore, silver ions alone and their chemically synthesised AgNPs can be used for various biomedical applications in future.

Inspec keywords: crops; microorganisms; scanning electron microscopy; ultraviolet spectra; nanofabrication; enzymes; silver; nanotechnology; X-ray diffraction; agricultural safety; plant diseases; biomedical materials; toxicology; nanoparticles; visible spectra; molecular biophysics

Other keywords: UV-visible spectroscopy; proline; biomedical applications; Ag; silver salt; sugarcane cultivation; scavenging reactive oxygen species; Saccharum spp; scanning electron microscopy; total phenolic content; superoxide dismutase; total flavonoid contents; X-ray diffraction analysis; proteins; malondialdehyde; catalase; silver nanoparticles; peroxidases activities; callus cultures; AgNO3; microbial disease resistance

Subjects: Engineering materials; Nanotechnology industry; Products and commodities; Agriculture; Health and safety aspects

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