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access icon free Autoclave-assisted synthesis of AgNPs in Z. officinale extract and assessment of their cytotoxicity, antibacterial and antioxidant activities

© The Institution of Engineering and Technology
Received 23/04/2018, Accepted 24/10/2018, Revised 01/10/2018, Published 20/11/2018

In this study, the authors synthesised silver nanoparticles (AgNPs) using autoclave as a simple, unique and eco-friendly approach. The effect of Zingiber officinale extract was evaluated as a reducing and stabiliser agent. According to transmission electron microscopy results, the AgNPs were in the spherical shape with a particle size of ∼17 nm. The biomedical properties of AgNPs as antibacterial agents and free radical scavenging activity were estimated. Synthesised AgNPs showed significant 1,1-diphenyl-2-picryl-hydrazyl free radical scavenging. Strong bactericidal activity was shown by the AgNPs on Gram-positive and Gram-negative bacteria. A maximum inhibition zone of ∼14 mm was obtained for epidermidis at a concentration of 60 μg/ml for sample fabricated at 24 h. The AgNPs also showed a significant cytotoxic effect against MCF-7 breast cancer cell lines with an half maximal inhibitory concentration value of 62 μg/ml in 24 h by the MTT assay. It could be concluded that Z. officinale extract can be used effectively in the production of potential antioxidant and antimicrobial AgNPs for commercial application.

Inspec keywords: nanomedicine; biochemistry; visible spectra; particle size; cancer; antibacterial activity; ultraviolet spectra; microorganisms; transmission electron microscopy; nanoparticles; silver; toxicology; organic compounds; free radicals; nanofabrication; biomedical materials; cellular biophysics

Other keywords: antimicrobial AgNPs; 1-diphenyl-2-picryl-hydrazyl free radical scavenging; silver nanoparticles; unique approach; free radical scavenging activity; cytotoxic effect; autoclave-assisted synthesis; eco-friendly approach; autoclave; transmission electron microscopy results; time 24.0 hour; strong bactericidal activity; reducing agent; antibacterial agents; synthesised AgNPs; antioxidant activities; zingiber officinale; stabiliser agent

Subjects: Products and commodities; Nanotechnology industry; Engineering materials; Industrial processes; Nanofabrication

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