One-pot green synthesis and structural characterisation of silver nanoparticles using aqueous leaves extract of Carissa carandas: antioxidant, anticancer and antibacterial activities

One-pot green synthesis and structural characterisation of silver nanoparticles using aqueous leaves extract of Carissa carandas: antioxidant, anticancer and antibacterial activities

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Facile green synthesis of silver nanoparticles (AgNPs) using an aqueous extract of Carissa carandas (C. carandas) leaves was studied. Fabrication of AgNPs was confirmed by the UV–visible spectroscopy which gives absorption maxima at 420 nm. C. carandas leaves are the rich source of the bioactive molecules, acts as a reducing and stabilising agent in AgNPs, confirmed by Fourier transforms infrared spectroscopy. The field emission scanning electron microscope revealed the spherical shape of biosynthesised AgNPs. A distinctive peak of silver at 3 keV was determined by energy dispersive X-ray spectroscopy. X-ray diffraction showed the facecentred cubic structure of biosynthesised AgNPs and thermal stability was confirmed by the thermogravimetric analysis. Total flavonoid and total phenolic contents were evaluated in biosynthesised AgNPs. Biosynthesised AgNPs showed free radical scavenging activities against 2, 2-diphenyl-1-picrylhydrazyl test and ferric reducing antioxidant power assay. In vitro cytotoxicity against hepatic cell lines (HUH-7) and renal cell lines (HEK-293) were also assessed. Finally, biosynthesised AgNPs were scrutinised for their antibacterial activity against methicillin-resistant Staphylococcus aureus, Shigella sonnei, Shigella boydii and Salmonella typhimurium. This study demonstrated the biofabrication of AgNPs by using C. carandas leaves extract and a potential in vitro biological application as antioxidant, anticancer and antibacterial agents.

Inspec keywords: field emission scanning electron microscopy; thermal analysis; nanofabrication; tumours; antibacterial activity; cellular biophysics; nanoparticles; free radical reactions; toxicology; reduction (chemical); nanomedicine; visible spectra; cancer; biomedical materials; microorganisms; Fourier transform infrared spectra; thermal stability; X-ray diffraction; silver; ultraviolet spectra; X-ray chemical analysis

Other keywords: field emission scanning electron microscope; renal cell lines HEK-293; free radical scavenging activities; anticancer activities; thermogravimetric analysis; hepatic cell lines HUH-7; ferric reducing antioxidant power assay; X-ray diffraction; Carissa carandas; methicillin-resistant Staphylococcus aureus; absorption maxima; biofabrication; distinctive peak; Shigella sonnei; total phenolic contents; reducing agent; antibacterial activity; one-pot green synthesis; silver nanoparticles; plant extract colour; face-centred cubic structure; energy dispersive X-ray spectroscopy; UV-visible spectroscopy; aqueous leaves extract; structural characterisation; Ag; antioxidant activities; bioactive molecules; thermal stability; total flavonoid contents; antibacterial activities; stabilising agent; in vitro biological application; 2,2-diphenyl-1-picrylhydrazyl test; Shigella boydii; Salmonella typhimurium; Fourier transforms infrared spectroscopy; in vitro cytotoxicity; spherical shape

Subjects: Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Optical properties of metals and metallic alloys (thin films/low-dimensional structures); Electromagnetic radiation spectrometry (chemical analysis); Biomedical materials; Nanotechnology applications in biomedicine; Visible and ultraviolet spectra of metals, semimetals, and alloys; Atom and radical reactions (with themselves or with molecules); Infrared and Raman spectra in metals; Cellular biophysics


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