Evaluating the effect of green synthesised copper oxide nanoparticles on oxidative stress and mitochondrial function using murine model

Evaluating the effect of green synthesised copper oxide nanoparticles on oxidative stress and mitochondrial function using murine model

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Green synthesis of metal nanoparticles (NPs) has now received the attention of researchers due to ease of preparation and its potential to overcome hazards of these chemicals for an eco-friendly milieu. In this study, copper oxide (CuO) NPs were synthesised via Desmodium gangeticum aqueous root extract and standard chemical method, further characterised by UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Thermogravimetric analysis and scanning electron microscopy. The nephrotoxicity of the NP obtained from two routes were compared and evaluated at subcellular level in Wistar rat, renal proximal epithelial cells (LLC PK1 cell lines) and isolated renal mitochondria. CuO NP synthesised by chemical route showed prominent nephrotoxicity measured via adverse cytotoxicity to LLC PK1 cells, elevated renal oxidative stress and damage to renal tissue (determined by impaired alanine transaminase, aspartate transaminase, urea, uric acid and creatinine in the blood). However, at the level of cell organelle, CuO NP from both routes are non-toxic to mitochondrial functional activity. The authors’ finding suggests that CuO NP synthesised by chemical route may induce nephrotoxicity, but may be overcome by co-administration of antioxidants, as it is not mito-toxic.

Inspec keywords: scanning electron microscopy; visible spectra; molecular biophysics; Fourier transform infrared spectra; nanoparticles; enzymes; biological tissues; nanomedicine; thermal analysis; nanofabrication; oxidation; cellular biophysics; copper compounds; antibacterial activity; biochemistry; X-ray diffraction; toxicology; blood; ultraviolet spectra

Other keywords: renal oxidative stress; nephrotoxicity; chemicals; murine model; impaired alanine transaminase; subcellular level; cytotoxicity; Fourier transform infrared spectroscopy; blood; copper oxide NPs; mitochondrial functional activity; renal proximal epithelial cells; cell organelle; standard chemical method; uric acid; scanning electron microscopy; Wistar rat; metal nanoparticles; X-ray diffraction; renal tissue; eco-friendly milieu; green synthesised copper oxide nanoparticles; aspartate transaminase; CuO; creatinine; urea; Desmodium gangeticum aqueous root extract; UV-visible spectroscopy; renal mitochondria; thermogravimetric analysis; LLC PK1 cell lines

Subjects: Low-dimensional structures: growth, structure and nonelectronic properties; Infrared and Raman spectra in inorganic crystals; Optical properties of other inorganic semiconductors and insulators (thin films/low-dimensional structures); Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Visible and ultraviolet spectra of other nonmetals; Physical chemistry of biomolecular solutions and condensed states; Biomedical materials; Nanotechnology applications in biomedicine; Cellular biophysics


    1. 1)
      • 1. Goel, S., Chen, F., Cai, W.: ‘Synthesis and biomedical applications of copper sulfide nanoparticles: from sensors to theranostics’, Small, 2014, 10, pp. 631645.
    2. 2)
      • 2. Maurer-Jones, M.A., Gunsolus, I.L., Murphy, C.J., et al: ‘Toxicity of engineered nanoparticles in the environment’, Analytical Chem., 2013, 85, (6), pp. 30363049.
    3. 3)
      • 3. Karlsson, H.L., Gliga, A.R., Calleja, F.M., et al: ‘Mechanism-based genotoxicity screening of metal oxide nanoparticles using the ToxTracker panel of reporter cell lines’, Part. Fibre Toxicol., 2014, 11, pp. 114.
    4. 4)
      • 4. Prabhu, B.M., Ali, S.F., Murdock, R.C., et al: ‘Copper nanoparticles exert size and concentration dependent toxicity on somatosensory neurons of rat’, Nanotoxicology, 2010, 4, pp. 150160.
    5. 5)
      • 5. Mekala, J., Rajan, M.R., Ramesh, R.: ‘Green synthesis and characterization of copper nanoparticles using tulsi (ocimum sanctum) leaf extract, PARIPEX’, Ind J. Res., 2016, 5, pp. 1416.
    6. 6)
      • 6. Lakshmi, K., Jayashree, M., Shakila Banu, K., et al: ‘Green and chemically synthesized copper oxide nanoparticles-A preliminary research towards its toxic behaviour’, Int. J. Pharm. Pharmaceut. Sci., 2015, 7, (13), pp. 156160.
    7. 7)
      • 7. FranÃğois, C., Fares, M., Baiocchi, C., et al: ‘Safety of desmodium adscendens extract on hepatocytes and renal cells. Protective effect against oxidative stress’, J. Intercult. Ethnopharmacol., 2015, 4, (1), pp. 15.
    8. 8)
      • 8. Zhou, J., Jin, J., Li, X., et al: ‘Total flavonoids of desmodium styracifolium attenuates the formation of hydroxy-l-proline-induced calcium oxalate urolithiasis in rats’, Urolithiasis, 2017, pp. 111,
    9. 9)
      • 9. Lee, I.-C., Ko, J.-W., Park, S.-H., et al: ‘Comparative toxicity and biodistribution of copper nanoparticles and cupric ions in rats’, Int. J. Nanomed., 2016, 11, pp. 28832900.
    10. 10)
      • 10. Khalil, M.M., Ismail, E.H., El-Baghdady, K.Z., et al: ‘Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity’, Arab. J. Chem., 2014, 7, (6), pp. 11311139.
    11. 11)
      • 11. Dang, T.M.D., Le, T.T.T., Fribourg-Blanc, E., et al: ‘Synthesis and optical properties of copper nanoparticles prepared by a chemical reduction method’, Adv. Nat. Sci. Nanosci. Nanotech., 2011, 2, (1), p. 015009.
    12. 12)
      • 12. Fraga, C.G., Leibovitz, B.E., Tappel, A.L.: ‘Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes’, Free Rad. Bio. Med., 1988, 4, pp. 155161.
    13. 13)
      • 13. Rotruck, J.T., Pope, A.L., Ganther, H.E., et al: ‘Selenium: biochemical role as a component of glutathione peroxidase’, Science, 1973, 179, p. 588.
    14. 14)
      • 14. Maehly, A.C., Chance, B.: ‘The assay of catalases and peroxidases’, Methods Biochem. Anal., 1954, 1, pp. 357424.
    15. 15)
      • 15. Marklund, S.L.: ‘Properties of extracellular superoxide dismutase from human lung’, Biochem. J., 1984, 220, (1), pp. 269272.
    16. 16)
      • 16. Bonner, W.D.: ‘Activation of the succinic dehydrogenase-cytochrome system’, Biochem. J., 1954, 56, (2), pp. 274285.
    17. 17)
      • 17. Kurian, G.A., Berenshtein, E., Kakhlon, O., et al: ‘Energy status determines the distinct biochemical and physiological behavior of interfibrillar and sub-sarcolemmal mitochondria’, Biochem. Biophys. Res. Commun., 2012, 428, (3), pp. 376382.
    18. 18)
      • 18. Rathi, A., Rao, C.V., Ravishankar, B., et al: ‘Anti-inflammatory and anti-nociceptive activity of the water decoction Desmodium gangeticum’, J. Ethnopharmacol., 2004, 95, pp. 259263.
    19. 19)
      • 19. Govindarajan, R., Rastogi, S., Vijayakumar, M., et al: ‘Studies on the antioxidant activities of Desmodium gangeticum’, Biol. Pharm. Bull., 2003, 26, pp. 14241427.
    20. 20)
      • 20. Kurian, G.A., Yagnesh, N., Kishan, R.S., et al: ‘Methanol extract of Desmodium gangeticum roots preserves mitochondrial respiratory enzymes, protecting rat heart against oxidative stress induced by reperfusion injury’, J. Pharm. Pharmacol., 2008, 60, pp. 523530.
    21. 21)
      • 21. Song, L., Vijver, M.G., Peijnenburg, W.J.: ‘Comparative toxicity of copper nanoparticles across three lemnaceae species’, Sci. Total. Environ., 2015, 518, pp. 217222.
    22. 22)
      • 22. Lemasters, J.J., Qian, T., He, L., et al: ‘Role of mitochondrial inner membrane permeabilization in necrotic cell death, apoptosis, and autophagy’, Antioxid. Redox. Signal., 2002, 4, pp. 769781.
    23. 23)
      • 23. Sankar, V., Pangayarselvi, B., Prathapan, A., et al: ‘Desmodium gangeticum (linn.) DC. Exhibits antihypertrophic effect in isoproterenol-induced cardiomyoblasts via amelioration of oxidative stress and mitochondrial alterations’, J. Cardiovasc. Pharmacol., 2013, 61, (1), pp. 2334.
    24. 24)
      • 24. Singh, S., D'Britto, V., Prabhune, A.A., et al: ‘Cytotoxic and genotoxic assessment of glycolipid-reduced and-capped gold and silver nanoparticles’, New J. Chem., 2010, 34, (2), pp. 294301.
    25. 25)
      • 25. Hauck, T.S., Ghazani, A.A., Chan, W.C.: ‘Assessing the effect of surface chemistry on gold nanorod uptake, toxicity, and gene expression in mammalian cells’, Small, 2008, 4, (1), pp. 153159.
    26. 26)
      • 26. Li, F., Lei, C., Shen, Q., et al: ‘Analysis of copper nanoparticles toxicity based on a stress-responsive bacterial biosensor array’, Nanoscale, 2013, 5, pp. 653662.

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