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access icon free Evaluation of potential anti-cancer activity of cationic liposomal nanoformulated Lycopodium clavatum in colon cancer cells

Research dealing with early diagnosis and efficient treatment in colon cancer to improve patient's survival is still under investigation. Chemotherapeutic agent result in high systemic toxicity due to their non-specific actions on DNA repair and/or cell replication. Traditional medicine such as Lycopodium clavatum (LC) has been claimed to have therapeutic potentials against cancer. The present study focuses on targeted drug delivery of cationic liposomal nanoformulated LC (CL-LC) in colon cancer cells (HCT15) and comparing the efficacy with an anti-colon cancer drug, 7-ethyl-10-hydroxy-camptothecin (SN38) along with its nanoformulated form (CL-SN38). The colloidal suspension of LC was made using thin film hydration method. The drugs were characterised using ultraviolet, dynamic light scattering, scanning electron microscopy, energy, dispersive X-ray spectroscopy. In vitro drug release showed kinetics of 49 and 89% of SN38 and LC, whereas CL-SN38 and CL-LC showed 73 and 74% of sustained drug release, respectively. Studies on morphological changes, cell viability, cytotoxicity, apoptosis, cancer-associated gene expression analysis of Bcl-2, Bax, p53 by real-time polymerase chain reaction and western blot analysis of Bad and p53 protein were performed. Nanoformulated LC significantly inhibited growth and increased the apoptosis of colon cancer cells indicating its potential anti-cancer activity against colon cancer cells.

Inspec keywords: colloids; genetics; cellular biophysics; DNA; nanostructured materials; light scattering; toxicology; X-ray chemical analysis; lipid bilayers; biological organs; drugs; suspensions; enzymes; drug delivery systems; nanomedicine; cancer; solvation; molecular biophysics; biochemistry

Other keywords: Lycopodium clavatum; scanning electron microscopy; apoptosis; energy dispersive X-ray spectroscopy; nanoformulated form CL-SN38; CL-LC; cationic liposomal nanoformulated LC; high systemic toxicity; western blot analysis; real-time polymerase chain reaction; anticolon cancer drug; cytotoxicity; cell viability; chemotherapeutic agents; in vitro drug release; colloidal suspension; DNA repair; therapeutic potentials; thin film hydration method; dynamic light scattering; patient survival; colon cancer diagnosis; colon cancer treatment; Bcl-2; ultraviolet scattering; Bax; cell replication; morphological changes; potential anticancer activity; cancer-associated gene expression analysis; p53 protein; colon cancer cells HCT15; Bad protein; targeted drug delivery; 7-ethyl-10-hydroxy-camptothecin; nonspecific actions; traditional medicines

Subjects: Association, addition, and insertion; Patient care and treatment; Interactions with radiations at the biomolecular level; Nanotechnology applications in biomedicine; Biomolecular structure, configuration, conformation, and active sites; Electronic structure and spectra of macromolecules; Patient care and treatment; Cellular biophysics; Biomolecular interactions, charge transfer complexes; Electromagnetic radiation spectrometry (chemical analysis); Macromolecular configuration (bonds, dimensions); Physical chemistry of biomolecular solutions and condensed states; Biomedical materials; Natural and artificial biomembranes; Colloids; Emulsions and suspensions

References

    1. 1)
      • 18. Jinachandran, K., Gunasekaran, A., Haseena, Y., et al: ‘Gene expression profile induced by liposomal nanoformulation of anticancer agents: insight into cell death mechanism’, Adv. Sci. Eng. Med., 2014, 6, (4), pp. 159165.
    2. 2)
      • 8. Pan, M.-H., Ho, C.-T.: ‘Chemopreventive effects of natural dietary compounds on cancer development’, Chem. Soc. Rev., 2008, 11, pp. 25582574.
    3. 3)
      • 9. Mohanraj, V.J., Chen, Y.: ‘Nanoparticles – a review’, Trop. J. Pharm. Res., 2005, 5, pp. 561573.
    4. 4)
      • 7. Waite, C.L., Roth, C.M.: ‘Nanoscale drug delivery systems for enhanced drug penetration into solid tumors: current progress and opportunities’, Crit. Rev. Biomed. Eng., 2012, 40, pp. 2141.
    5. 5)
      • 12. Bisht, S., Maitra, A.: ‘Dextran–doxorubicin/chitosan nanoparticles for solid tumor therapy’, Wiley Interdiscip. Rev., Nanomed. Nanobiotechnol., 2009, 14, pp. 415425.
    6. 6)
      • 11. Essack, M., Bajic, V.B., Archer, J.A.: ‘Recently confirmed apoptosis-inducing lead compounds isolated from marine sponge of potential relevance in cancer treatment’, Mar. Drugs, 2011, 9, pp. 15801606.
    7. 7)
      • 14. Orhan, I., Küpeli, E., Şener, B., et al: ‘Appraisal of anti-inflammatory potential of the club moss Lycopodium clavatum L.’, J. Ethnopharmacol., 2007, 109, pp. 146150.
    8. 8)
      • 3. Fearon, E.R., Vogelstein, B.: ‘A genetic model for colorectal tumorigenesis’, Cell, 1990, 3, pp. 759767.
    9. 9)
      • 16. Lawrence, G.H.M.: ‘Taxonomy of vascular plants’, vol. 1989 (MacMillan Publishing Co., New York, USA, 1951), pp. 337338.
    10. 10)
      • 5. Singh, S.K., Hawkins, C., Clarke, I.D., et al: ‘Identification of human brain tumour initiating cells’, Nature, 2004, 7015, pp. 396401.
    11. 11)
      • 13. Chang, H.R.: ‘Trastuzumab based neoadjuvant therapy in patients with HER2 positive breast cancer’, Cancer, 2010, 116, pp. 28562867.
    12. 12)
      • 15. Corns, C.M.: ‘Herbal remedies and clinical biochemistry’, Ann. Clin. Biochem., 2003, 40, pp. 489507.
    13. 13)
      • 1. Ilyas, M., Straub, J., Tomlinson, I.P., et al: ‘Genetic pathways in colorectal and other cancers’, Eur. J. Cancer, 1999, 14, pp. 19862002.
    14. 14)
      • 10. Kehrer, D.F.S., Soepenberg, O., Loos, W.J., et al: ‘Modulation of camptothecin analogs in the treatment of cancer: a review’, Anti-cancer Drugs, 2001, 12, pp. 89105.
    15. 15)
      • 4. Bonnet, D., Dick, J.E.: ‘Human acute myeloid leukemia is organize as a hierarchy that originates from a primitive hematopoietic cell’, Nat. Med., 1997, 3, pp. 730737.
    16. 16)
      • 6. Cairns, R., Papandreou, I., Denko, N.: ‘Overcoming physiologic barriers to cancer treatment by molecularly targeting the tumor microenvironment’, Mol. Cancer Res., 2006, 4, pp. 6170.
    17. 17)
      • 2. Pardal, R., Clarke, M.K., Morrison, M.J.: ‘Applying the principles of stem-cell biology to cancer’, Nat. Rev. Can., 2003, 3, pp. 895902.
    18. 18)
      • 17. Mandal, S.K., Biswas, R., Bhattacharyya, S.S., et al: ‘Lycopodine from Lycopodium clavatum extract inhibits proliferation of HeLa cells through induction of apoptosis via caspase-3 activation’, Eur. J. Pharmacol., 2010, 626, pp. 115122.
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