access icon free Diospyros assimilis root extract assisted biosynthesised silver nanoparticles and their evaluation of antimicrobial activity

© The Institution of Engineering and Technology
Received 21/02/2017, Accepted 05/09/2017, Revised 02/08/2017, Published 12/09/2017

The current research study focuses on biosynthesis of silver nanoparticles (Ag NPs) for the first time from silver acetate employing methanolic root extract of Diospyros assimilis. The UV–Vis absorption spectrum of biologically synthesised nanoparticles displayed a surface plasmon peak at 428 nm indicating the formation of Ag NPs. The influence of metal ion concentration, reaction time and amount of root extract in forming Ag NPs by microscopic and spectral analysis was thoroughly investigated. Structural analysis from transmission electron microscopy confirmed the nature of metallic silver as face-centered cubic (FCC) crystalline with an average diameter of 17 nm, which correlates with an average crystallite size (19 nm) calculated from X-ray diffraction analysis. Further, the work was extended for the preliminary examination of antimicrobial activity of biologically synthesised Ag NPs that displayed promising activity against all the tested pathogenic strains.

Inspec keywords: surface plasmon resonance; optical microscopy; silver; transmission electron microscopy; nanomedicine; crystallites; particle size; nanofabrication; X-ray diffraction; antibacterial activity; nanoparticles; visible spectra; biomedical materials; microorganisms; ultraviolet spectra

Other keywords: spectral analysis; structural analysis; methanolic root extract; UV-visible absorption spectrum; reaction time; average crystallite size; metallic silver; transmission electron microscopy; FCC crystalline phase; Diospyros assimilis root extract assisted biosynthesised silver nanoparticles; antimicrobial activity; Ag; Ag NPs formation; silver acetate; biologically synthesised nanoparticles; surface plasmon peak; microscopic analysis; X-ray diffraction analysis; metal ion concentration; pathogenic strains

Subjects: Nanotechnology applications in biomedicine; Visible and ultraviolet spectra of metals, semimetals, and alloys; Biomedical materials; Low-dimensional structures: growth, structure and nonelectronic properties; Optical properties of metals and metallic alloys (thin films, low-dimensional and nanoscale structures); Preparation of metals and alloys (compacts, pseudoalloys); Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Collective excitations (surface states); Other methods of nanofabrication; Microstructure

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