access icon free Bacterial detection based on polymerase chain reaction and microbead dielectrophoresis characteristics

© The Institution of Engineering and Technology
Received 13/09/2016, Accepted 02/12/2016, Revised 08/11/2016, Published 07/12/2016

In this study, an electrical DNA detection method was applied to bacterial detection. DNA was extracted from bacteria and amplified by polymerase chain reaction. The microbeads were labelled with amplicons, altering their surface conductance and therefore their dielectrophoresis characteristics. Amplicon-labelled microbeads could thus be trapped within a high-strength electric field, where they formed a pearl chain between the electrodes, resulting in an increased conductance between the electrodes. This method reduces the amplicon detection time from 1–2 h to 15 min, compared with the conventional method. The presented method realised quantitative detection of specific bacteria at concentrations above 1 × 105 and 2.4 × 104 CFU/ml for bacterial solutions with and without other bacterial presence, respectively.

Inspec keywords: bioMEMS; electrochemical electrodes; electrophoresis; molecular biophysics; surface conductivity; DNA; microorganisms; electrochemical sensors; biosensors; microsensors; enzymes; bioelectric phenomena; biochemistry

Other keywords: electrodes; amplicon detection time; pearl chain; amplicon-labelled microbeads; surface conductance; high-strength electric field; bacterial detection; bacterial solutions; electrical DNA detection; microbead dielectrophoresis characteristics; quantitative detection; polymerase chain reaction; time 15 min to 2 h

Subjects: Biosensors; Biosensors; Bioelectricity; Microsensors and nanosensors; Fabrication of MEMS and NEMS devices; Electrochemical analytical methods; Physical chemistry of biomolecular solutions and condensed states; Biological engineering and techniques; Electrochemistry and electrophoresis; Chemical sensors; Micromechanical and nanomechanical devices and systems; Chemical sensors; Biomolecular interactions, charge transfer complexes; Chemical variables measurement; Sensing and detecting devices; Biomolecular structure, configuration, conformation, and active sites

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