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access icon free Facile synthesis of silver nanoparticles mediated by polyacrylamide-reduction approach to antibacterial application

  • Author(s): Hosam I. Salaheldin 1, 2 ; Meshal H.K. Almalki 3 ; Abd Elhameed.M. Hezma 4 ; Gamal E.H. Osman 3, 5
    • View affiliations
    • Affiliations: 1: Department of Physics, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, PO Box 715, Kingdom of Saudi Arabia ;
      2: Department of Physics, Faculty of Science, Mansoura University, Mansoura 35516, Egypt ;
      3: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, PO Box 715, Kingdom of Saudi Arabia ;
      4: Spectroscopy Department, Physics Division, National Research Centre, Dokki, 12622 Giza, Egypt ;
      5: Microbial Genetics Department, Agricultural Genetic Engineering Research Institute (AGERI), Giza 12619, Egypt
  • Source: Volume 11, Issue 4, June 2017, p. 448 – 453
    DOI:  10.1049/iet-nbt.2016.0135 , Print ISSN 1751-8741, Online ISSN 1751-875X
© The Institution of Engineering and Technology
Received 28/06/2016, Accepted 24/10/2016, Revised 04/10/2016, Published 25/10/2016

The current time increase in the prevalence of antibiotic resistant ‘super-bugs’ and the risks associated with food safety have become global issues. Therefore, further research is warranted to identify new and effective antimicrobial substances. Silver nanoparticles (Ag-NPs) were synthesized by autoclaving technique using, different concentrations of Ag salt (AgNO3) solution (1, 5, 10, and 25 mM). Their presence was confirmed by a surface plasmon resonance band at ∼435 nm using UV–Vis absorption spectra. The morphology of the synthesized Ag-NPs stabilized by polyacrylamide (PAM) was examined by TEM, SAED, and EDS. TEM images revealed that the synthesized Ag-NPs had an average diameter of 2.98±0.08 nm and SAED and EDS results confirmed the formation of Ag-NPs. In addition, FT-IR spectroscopy revealed that a PAM polymer matrix stabilized the Ag-NPs. The well diffusion method, was used to test, Gram positive and Gram negative bacteria were examined. Also the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were studied against Ag-NPs. The Ag-NPs exhibited strong inhibitory activity, MIC and MBC against the tested clinical bacterial isolates. These results suggest that Ag-NPs stabilized in PAM are highly effective against clinical bacterial isolates can be applied in medical fields.

Inspec keywords: silver; antibacterial activity; nanomedicine; transmission electron microscopy; nanoparticles; electron diffraction; Fourier transform infrared spectroscopy; ultraviolet spectra; surface plasmon resonance; X-ray chemical analysis; microorganisms; visible spectra

Other keywords: electron diffraction; ultraviolet-visible absorption spectra; Ag; clinical bacterial isolates; antibiotic resistant super-bugs; transmission electron microscopy; diffusion method; Ag-NP facile synthesis; Gram positive bacteria; antibiotics; Ag salt solution concentration; TEM images; PAM polymer matrix; food safety; Gram negative bacteria; energy dispersive X-ray spectroscopy; Fourier transform infrared spectroscopy; polyacrylamide; antimicrobial agents; PAM-reduction approach; antibacterial application; antimicrobial substances

Subjects: Nanotechnology applications in biomedicine

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