Biofabrication of broad range antibacterial and antibiofilm silver nanoparticles

Author(s): Shariq Qayyum and Asad Ullah Khan
DOI: 10.1049/iet-nbt.2015.0091

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Author(s): Shariq Qayyum ¹ and Asad Ullah Khan ¹
- Affiliations: 1: Medical Microbiology and Molecular Biology, Laboratory Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
Source: Volume 10, Issue 5, October 2016, p. 349 – 357
DOI: 10.1049/iet-nbt.2015.0091 , Print ISSN 1751-8741, Online ISSN 1751-875X

Silver nanoparticles (AgNPs) were biosynthesized via a green route using ten different plants extracts (GNP1- Caryota urens, GNP2-Pongamia glabra, GNP3- Hamelia patens, GNP4-Thevetia peruviana, GNP5-Calendula officinalis, GNP6-Tectona grandis, GNP7-Ficus petiolaris, GNP8- Ficus busking, GNP9- Juniper communis, GNP10-Bauhinia purpurea). AgNPs were tested against drug resistant microbes and their biofilms. These nanoparticles (NPs) were characterised using UV-vis spectroscopy, transmission electron microscope, Fourier transform infrared spectroscopy, X-ray diffraction and Image J software. Most of the AgNPs were distributed over a range of 1 of 60 nm size. The results indicated that AgNPs were antibacterial in nature without differentiating between resistant or susceptible strains. Moreover, the effect was more prominent on Gram negative bacteria then Gram positive bacteria and fungus. AgNPs inhibited various classes of microbes with different concentration. It was also evident from the results that the origin or nature of extract did not affect the activity of the NPs. Protein and carbohydrate leakage assays confirmed that the cells lysis is one of the main mechanisms for the killing of microbes by green AgNPs. This study suggests that the action of AgNPs on microbial cells resulted into cell lysis and DNA damage. Excellent microbial biofilm inhibition was also seen by these green AgNPs. AgNPs have proved their candidature as a potential antibacterial and antibiofilm agent against MDR microbes.

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Biofabrication of broad range antibacterial and antibiofilm silver nanoparticles

Biofabrication of broad range antibacterial and antibiofilm silver nanoparticles

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