Plant-mediated green synthesis of silver nanoparticles using Trifolium resupinatum seed exudate and their antifungal efficacy on Neofusicoccum parvum and Rhizoctonia solani

Mehrdad Khatami; Meysam Soltani Nejad; Samira Salari; Pooya Ghasemi Nejad Almani

Plant-mediated green synthesis of silver nanoparticles using Trifolium resupinatum seed exudate and their antifungal efficacy on Neofusicoccum parvum and Rhizoctonia solani

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Author(s): Mehrdad Khatami ¹ ; Meysam Soltani Nejad ² ; Samira Salari ^{3, 4} ; Pooya Ghasemi Nejad Almani ³
- Affiliations: 1: Department of Biotechnology, Shahid Bahonar University of Kerman, Kerman, Iran ;
  2: Department of Plant Pathology, Shahid Bahonar University of Kerman, Kerman, Iran ;
  3: Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran ;
  4: Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
Source: Volume 10, Issue 4, August 2016, p. 237 – 243
DOI: 10.1049/iet-nbt.2015.0078 , Print ISSN 1751-8741, Online ISSN 1751-875X

Received 05/10/2015, Accepted 14/12/2015, Revised 24/11/2015, Published 01/08/2016

In recent years, biosynthesis and the utilisation of silver nanoparticles (AgNPs) has become an interesting subject. In this study, the authors investigated the biosynthesis of AgNPs using Trifolium resupinatum (Persian clover) seed exudates. The characterisation of AgNPs were analysed using ultraviolet–visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infra-red spectroscopy. Also, antifungal efficacy of biogenic AgNPs against two important plant-pathogenic fungi (Rhizoctonia solani and Neofusicoccum Parvum) in vitro condition was evaluated. The XRD analysis showed that the AgNPs are crystalline in nature and have face-centred cubic geometry. TEM images revealed the spherical shape of the AgNPs with an average size of 17 nm. The synthesised AgNPs were formed at room temperature and kept stable for 4 months. The maximum distributions of the synthesised AgNPs were seen to range in size from 5 to 10 nm. The highest inhibition effect was observed against R. solani at 40 ppm concentration of AgNPs (94.1%) followed by N. parvum (84%). The results showed that the antifungal activity of AgNPs was dependent on the amounts of AgNPs. In conclusion, the AgNPs obtained from T. resupinatum seed exudate exhibit good antifungal activity against the pathogenic fungi R. solani and N. Parvum.

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Errata: Plant-mediated green synthesis of silver nanoparticles using Trifolium resupinatum seed exudate and their antifungal efficacy on Neofusicocum parvum and Rhizoctonia solani

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Plant-mediated green synthesis of silver nanoparticles using Trifolium resupinatum seed exudate and their antifungal efficacy on Neofusicoccum parvum and Rhizoctonia solani

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