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Interaction of gold nanoparticles with Pfu DNA polymerase and effect on polymerase chain reaction

Interaction of gold nanoparticles with Pfu DNA polymerase and effect on polymerase chain reaction

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The interaction of gold nanoparticles with Pfu DNA polymerase has been investigated by a number of biological, optical and electronic spectroscopic techniques. Polymerase chain reaction was performed to show gold nanoparticles’ biological effect. Ultraviolet–visible and circular dichroism spectra analysis were applied to character the structure of Pfu DNA polymerase after conjugation with gold nanoparticles. X-ray photoelectron spectroscopy was used to investigate the bond properties of the polymerase-gold nanoparticles complex. The authors demonstrate that gold nanoparticles do not affect the amplification efficiency of polymerase chain reaction using Pfu DNA polymerase, and Pfu DNA polymerase displays no significant changes of the secondary structure upon interaction with gold nanoparticles. The adsorption of Pfu DNA polymerase to gold nanoparticles is mainly through Au–NH2 bond and electrostatic interaction. These findings may have important implications regarding the safety issue as gold nanoparticles are widely used in biomedical applications.

Inspec keywords: molecular configurations; electron spectroscopy; nanobiotechnology; visible spectra; X-ray photoelectron spectra; ultraviolet spectra; circular dichroism; DNA; bonds (chemical); spectrochemical analysis; molecular biophysics; biochemistry; gold; biological techniques; adsorption; nanoparticles

Other keywords: X-ray photoelectron spectroscopy; biological technique; secondary structure; Au; electrostatic interaction; adsorption; electronic spectroscopic technique; circular dichroism spectra analysis; biomedical applications; optical technique; Pfu DNA polymerase; polymerase chain reaction; amplification efficiency; conjugation; ultraviolet-visible spectra analysis; bond properties; polymerase-gold nanoparticles complex

Subjects: Macromolecular configuration (bonds, dimensions); Biophysical instrumentation and techniques; Biomolecular structure, configuration, conformation, and active sites; X-ray photoelectron spectra of molecules; Electromagnetic radiation spectrometry (chemical analysis); Electron spectroscopy for chemical analysis (photoelectron, Auger spectroscopy, etc.); Biomolecular interactions, charge transfer complexes; Physical chemistry of biomolecular solutions and condensed states; Optical activity, optical rotation, circular dichroism in molecules; Interactions with radiations at the biomolecular level; Sorption and accommodation coefficients (surface chemistry)

References

    1. 1)
      • M. Li , Y.C. Lin , C.C. Wu , H.S. Liu . Enhancing the efficiency of a PCR using gold nanoparticles. Nucl. Acids Res.
    2. 2)
      • B.V. Vu , D. Litvinov , R.C. Willson . Gold nanoparticle effects in polymerase chain reaction: favoring of smaller products by polymerase adsorption. Anal. Chem. , 5462 - 5467
    3. 3)
      • L.J. Mi , H.P. Zhu , X.D. Zhang , J. Hu , C.H. Fan . Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR. Chin. Sci. Bull. , 2345 - 2349
    4. 4)
      • W. Zhao , F. Gonzaga , Y.F. Li , A. Michael . Brook highly stabilized nucleotide-capped small gold nanoparticles with tunable size. Adv. Mater. , 1766 - 1771
    5. 5)
      • L.J. Lai , Y.W. Yang , Y.K. Lin , L.L. Huang , Y.H. Hsieh . Surface characterization of immunosensor conjugated with gold nanoparticles based on cyclic voltammetry and X-ray photoelectron spectroscopy. Colloid Surf. B – Biointerfaces , 130 - 135
    6. 6)
      • J. Cline , J.C. Braman , H.H. Hogrefe . PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases. Nucl. Acids Res. , 3546 - 3551
    7. 7)
      • J.H. Huang , X.D. Zhang , C.M. Wang . Size and surface effect of gold nanoparticles (AuNPs) in nanogold-assisted PCR. Surf. Rev. Lett. , 757 - 762
    8. 8)
      • H.K. Li , J.H. Huang , J.H. Lv . Nanoparticle PCR: nanogold-assisted PCR with enhanced specificity. Angew. Chem. Int. Edit. , 5100 - 5103
    9. 9)
      • S.W. Kim , D.U. Kim , J.K. Kim , L.W. Kang , H.S. Cho . Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus. Int. J. Biol. Macromol. , 356 - 361
    10. 10)
      • P.H. Patel , L.A. Loeb . DNA polymerase active site is highly mutable: evolutionary consequences. PNAS , 5095 - 5100
    11. 11)
      • L.V. Gening , S.A. Klincheva , A. Reshetnjak , A.P. Grollman , H. Miller . RNA aptamers selected against DNA polymerase β inhibit the polymerase activities of DNA polymerases β and κ. Nucl. Acids Res. , 2579 - 2586
    12. 12)
      • T. Sainsbury , T. Ikuno , D. Okawa , D. Pacile , J.M.J. Frechet , A. Zettl . Self-assembly of gold nanoparticles at the surface of amine- and thiol-functionalized boron nitride nanotubes. J. Phys. Chem. C , 12992 - 12999
    13. 13)
      • N. Wangoo , C.R. Suri , G. Shekhawat . Interaction of gold nanoparticles with protein: a spectroscopic study to monitor protein conformational changes. Appl. Phys. Lett. , 133104 - 133106
    14. 14)
      • W. Yang , L. Mi , X. Cao , X. Zhang , C. Fan , J. Hu . Evaluation of gold nanoparticles as the additive in real-time polymerase chain reaction with SYBR Green I dye. Nanotechnology , 255101 - 255108
    15. 15)
      • E. Boisselier , D. Astruc . Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem. Soc. Rev. , 1759 - 1782
    16. 16)
      • L.P. Sun , Z.W. Zhang , S. Wang . Effect of pH on the interaction of gold nanoparticles with DNA and application in the detection of human p53 gene mutation. Nanoscale Res. Lett. , 216 - 220
    17. 17)
      • H.P. Zhu , L.J. Mi , S.M. Chen , W.F. Wang , S.D. Yao . Study on the interaction of colloidal gold with Taq DNA polymerase. Chin. J. Chem. , 1233 - 1237
    18. 18)
      • J.J. Storhoff , R.C. Mucic , C.A. Mirkin , R.L. Letsinger , G.C. Schatz , A.A. Lazarides . What controls the optical properties of DNA-linked gold nanoparticle assemblies?. J. Am. Chem. Soc. , 4640 - 4650
    19. 19)
      • Sun, Z.H.: `Purification of recombinant DNA polymerase in ', 2006, Master, Zhejiang University, China.
    20. 20)
      • H.K. Li , X.Y. Cao , Z.L. Zhou , H. Chen . Preliminary research on gold nanoparticles enhancing the specificity of PCR amplification of low copy gene from the complicated system. China Biotechnol. , 75 - 80
    21. 21)
      • Y. Kim , S.H. Eom , J. Wang , D.S. Lee , S.W. Suh , T.A. Steitz . Crystal structure of Thermus aquaticus DNA polymerase. Nature , 612 - 616
    22. 22)
      • W.J. Wan , J.T.W. Yeow . The effects of gold nanoparticles with different sizes on polymerase chain reaction efficiency. Nanotechnology , 325702 - 325706
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