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access icon openaccess Production of recombinant collagen: state of the art and challenges

This is an open access article published by the IET under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/)
Received 24/02/2017, Accepted 24/04/2017, Revised 30/03/2017, Published 11/07/2017

Collagen, which is often used in healthcare materials and biomedical research, is largely extracted from animal sources. Recombinant human collagen has the potential to be a promising alternative to animal collagen which has many shortcomings, including immunogenicity and lack of biocompatibility. Currently, recombinant human collagen has been expressed in both eukaryotic and prokaryotic hosts with varying degrees of success. One issue with recombinant collagen across all hosts is the inability to achieve full length collagen with native amounts of post-translational modifications, prompting much exciting research in this direction. There has also been much effort in improving yield and biomimicry of recombinant collagen. This review discusses collagen structure and current methods for extracting animal collagen, before introducing current research in synthesising recombinant human collagen in various hosts, and finally highlighting challenges in the field.

Inspec keywords: molecular biophysics; proteins; biochemistry; biomimetics; biomedical materials

Other keywords: biomedical research; biocompatibility; biomimicry; recombinant human collagen; collagen structure; animal sources; eukaryotic hosts; animal collagen; prokaryotic hosts; immunogenicity; healthcare materials

Subjects: Biomolecular structure, configuration, conformation, and active sites; Biomedical materials; Physical chemistry of biomolecular solutions and condensed states

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