Identification and classification of acute lymphoblastic leukemia cells from peripheral blood by using Raman spectroscopy
Identification and classification of acute lymphoblastic leukemia cells from peripheral blood by using Raman spectroscopy
- Author(s): S. Manago ; C. Valente ; P. Mirabelli ; M. Napolitano ; D. Corda ; A.C. De Luca
- DOI: 10.1049/cp.2016.0938
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- Author(s): S. Manago ; C. Valente ; P. Mirabelli ; M. Napolitano ; D. Corda ; A.C. De Luca Source: 18th Italian National Conference on Photonic Technologies (Fotonica 2016), 2016 page ()
- Conference: 18th Italian National Conference on Photonic Technologies (Fotonica 2016)
- DOI: 10.1049/cp.2016.0938
- ISBN: 978-1-78561-268-8
- Location: Rome, Italy
- Conference date: 6-8 June 2016
- Format: PDF
In this work we have used the Raman spectroscopy to identify and classify normal B cells, collected from different healthy patients, versus three different B acute lymphoblastic leukemia (B-ALL) cell lines (RS4;11, REH and MN60), by analyzing their biochemical features. Moreover, we have studied the effects of low-dose treatments of methotrexate (MTX), a key drug generally used in the BALL maintenance therapy, and all-trans-retinoic acid (ATRA), a drug used in the maintenance therapy of a different leukemic disease [1]. We have found a regression of the disease in the three B-ALL cell lines after MTX-treatments, while there were no effects after ATRA treatments. In order to quantify the capability of our system to classify leukemia/normal cells, we performed Principal Component Analysis (PCA) and obtained an accuracy of 97% for differentiating between three leukemia cell lines. In addition, we obtained a high accuracy (about 99%) in the classification of normal B cells versus the three B-ALL cell lines after MTX-treatments.
Inspec keywords: Raman spectra; patient treatment; drugs; cellular biophysics; blood; biochemistry
Subjects: Patient care and treatment; Cellular biophysics; Physical chemistry of biomolecular solutions and condensed states
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