A method for fabricating patterned deposition of silver nanoparticles on the surface of a standard glass slide using laser-induced way is reported. By this method patterned deposition of Ag nanoparticles corresponding to a given irradiation pattern can be achieved. In this paper focused laser light, with a concentric circle pattern formed by a confocal microscope, in an aqueous solution of silver nitrate and trisodium citrate allowed the synthesis and immobilization of silver nanoparticles at the glass/solution interface. Microscope image demonstrated that the deposition distribution of silver nanoparticles was in good accordance with irradiation pattern. Meanwhile Scanning Electron Microscope (SEM) images illustrate that the synthesis of Ag nanoparticles only occurred in the irradiated area, and with increase of irradiation time, the number and size of Ag nanoparticles increased, and what is more, the shape of these nanoparticles changed from granule to flake. The as-synthesized Ag nanoparticles were free from aggregation. Irradiation power is a factor through which we can change the shape of nanoparticles. (3 pages)