This chapter discusses a new type of spectroscopy instrument for recording protein spectra using thermally modulated fluorescence, which would have to be constructed and a microwave swept signal generator and amplifier would need to be obtained.

Chapter Contents:

• 10.1 Microwave spectroscopy for caged proteins
• 10.2 Microwave spectra for Blue Fluorescent Protein
• 10.3 Matching frequencies
• 10.4 Estimating parameters and sensitivity
• 10.5 Arginine and lysine hot spots
• 10.6 Calcium oscillators - microwave sensitivity
• 10.7 The first excited vibrational state
• 10.8 Mode switching at infrared frequencies

Inspec keywords: microwave spectroscopy; fluorescence; microwave amplifiers; signal generators; fluorescence spectroscopy; molecular biophysics; proteins

Other keywords: microwave swept signal amplifier; thermally modulated fluorescence; microwave swept signal generator; spectroscopy instrument; protein spectra recording; microwave thermal fluorescence spectroscopy

Subjects: Electronic structure and spectra of macromolecules; Amplifiers; Magnetic resonance spectrometers, auxiliary instruments and techniques; Interactions with radiations at the biomolecular level; Signal generators; Biological engineering and techniques; Biophysical instrumentation and techniques