IET Nanobiotechnology
Volume 8, Issue 3, September 2014
Volumes & issues:
Volume 8, Issue 3
September 2014
Application of silica nanoparticles in maize to enhance fungal resistance
- Author(s): Rangaraj Suriyaprabha ; Gopalu Karunakaran ; Kandiah Kavitha ; Rathinam Yuvakkumar ; Venkatachalam Rajendran ; Narayanasamy Kannan
- Source: IET Nanobiotechnology, Volume 8, Issue 3, p. 133 –137
- DOI: 10.1049/iet-nbt.2013.0004
- Type: Article
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In this study, maize treated with nanosilica (20–40 nm) is screened for resistance against phytopathogens such as Fusarium oxysporum and Aspergillus niger and compared with that of bulk silica. The resistivity is measured for disease index and expression of plant responsive compounds such as total phenols, phenylalanine ammonia lyase, peroxidase and polyphenol oxidase. The results indicate that nanosilica-treated plant shows a higher expression of phenolic compounds (2056 and 743 mg/ml) and a lower expression of stress-responsive enzymes against both the fungi. Maize expresses more resistance to Aspergillus spp., than Fusarium spp. These results show significantly higher resistance in maize treated with nanosilica than with bulk, especially at 10 and 15 kg/ha. In addition, hydrophobic potential and silica accumulation percentage of nanosilica treated maize (86.18° and 19.14%) are higher than bulk silica treatment. Hence, silica nanoparticles can be used as an alternative potent antifungal agent against phytopathogens.
Ionic selectivity of nystatin A1 confined in nanoporous track-etched polymer membrane
- Author(s): Sébastien Balme ; Daniela Thiele ; Sebastian Kraszewski ; Fabien Picaud ; Jean-Marc Janot ; Philippe Déjardin
- Source: IET Nanobiotechnology, Volume 8, Issue 3, p. 138 –142
- DOI: 10.1049/iet-nbt.2013.0014
- Type: Article
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The hybrid biological/polymeric solid-state nanopore membrane offers several opportunities to combine the advantage of biological channel (selectivity) and material (robustness). Based on this technology, the challenge is to obtain selective ionic exchange membranes, with no energy intake. The direct insertion of an ionic channel inside a nanopore should be a promise solution. Here, the authors report a hybrid nanopore based on nystatin A1 confinement in commercial nanopore membrane. Ionic transport and selectivity studies show that the hybrid nanopores exhibit mainly an anionic behaviour, on the contrary to biological conditions. However, the order of magnitude between the different ratios of permeation of several cationic species is retained even if the blocking of divalent cation is not totally proved.
Boron nitride nanotube-based biosensing of various bacterium/viruses: continuum modelling-based simulation approach
- Author(s): Mitesh B. Panchal and Sanjay H. Upadhyay
- Source: IET Nanobiotechnology, Volume 8, Issue 3, p. 143 –148
- DOI: 10.1049/iet-nbt.2013.0020
- Type: Article
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In this study, the feasibility of single walled boron nitride nanotube (SWBNNT)-based biosensors has been ensured considering the continuum modelling-based simulation approach, for mass-based detection of various bacterium/viruses. Various types of bacterium or viruses have been taken into consideration at the free-end of the cantilevered configuration of the SWBNNT, as a biosensor. Resonant frequency shift-based analysis has been performed with the adsorption of various bacterium/viruses considered as additional mass to the SWBNNT-based sensor system. The continuum mechanics-based analytical approach, considering effective wall thickness has been considered to validate the finite element method (FEM)-based simulation results, based on continuum volume-based modelling of the SWBNNT. As a systematic analysis approach, the FEM-based simulation results are found in excellent agreement with the analytical results, to analyse the SWBNNTs for their wide range of applications such as nanoresonators, biosensors, gas-sensors, transducers and so on. The obtained results suggest that by using the SWBNNT of smaller size the sensitivity of the sensor system can be enhanced and detection of the bacterium/virus having mass of 4.28 × 10−24 kg can be effectively performed.
Single walled boron nitride nanotube-based biosensor: an atomistic finite element modelling approach
- Author(s): Mitesh B. Panchal and Sanjay H. Upadhyay
- Source: IET Nanobiotechnology, Volume 8, Issue 3, p. 149 –156
- DOI: 10.1049/iet-nbt.2013.0012
- Type: Article
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The unprecedented dynamic characteristics of nanoelectromechanical systems make them suitable for nanoscale mass sensing applications. Owing to superior biocompatibility, boron nitride nanotubes (BNNTs) are being increasingly used for such applications. In this study, the feasibility of single walled BNNT (SWBNNT)-based bio-sensor has been explored. Molecular structural mechanics-based finite element (FE) modelling approach has been used to analyse the dynamic behaviour of SWBNNT-based biosensors. The application of an SWBNNT-based mass sensing for zeptogram level of mass has been reported. Also, the effect of size of the nanotube in terms of length as well as different chiral atomic structures of SWBNNT has been analysed for their sensitivity analysis. The vibrational behaviour of SWBNNT has been analysed for higher-order modes of vibrations to identify the intermediate landing position of biological object of zeptogram scale. The present molecular structural mechanics-based FE modelling approach is found to be very effectual to incorporate different chiralities of the atomic structures. Also, different boundary conditions can be effectively simulated using the present approach to analyse the dynamic behaviour of the SWBNNT-based mass sensor. The presented study has explored the potential of SWBNNT, as a nanobiosensor having the capability of zeptogram level mass sensing.
Irreversible magnetoporation of micro-organisms in high pulsed magnetic fields
- Author(s): Vitalij Novickij ; Audrius Grainys ; Jurij Novickij ; Svetlana Markovskaja
- Source: IET Nanobiotechnology, Volume 8, Issue 3, p. 157 –162
- DOI: 10.1049/iet-nbt.2013.0005
- Type: Article
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Electroporation is an appealing way of stimulating living cells, which causes permanent or temporary nanoporosities in the structure of the biological objects. However, the technique has a disadvantage such as a requirement of contact between the electrodes and the cell medium. In this review, a methodology of contactless treatment of the biological objects using pulsed magnetic fields is proposed. The eukaryotic micro-organisms Achlya americana and Saprolegnia diclina have been used in the study and magnetic fields up to 7 T were applied, which caused effects similar to irreversible electroporation resulting in the death of the species. The proposed technique is applicable for different types of the biological cells or micro-organisms and possibly can be used in the area of cancer, antifungal treatment and other biotechnological fields.
Field-programmable lab-on-a-chip based on microelectrode dot array architecture
- Author(s): Gary Wang ; Daniel Teng ; Yi-Tse Lai ; Yi-Wen Lu ; Yingchieh Ho ; Chen-Yi Lee
- Source: IET Nanobiotechnology, Volume 8, Issue 3, p. 163 –171
- DOI: 10.1049/iet-nbt.2012.0043
- Type: Article
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The fundamentals of electrowetting-on-dielectric (EWOD) digital microfluidics are very strong: advantageous capability in the manipulation of fluids, small test volumes, precise dynamic control and detection, and microscale systems. These advantages are very important for future biochip developments, but the development of EWOD microfluidics has been hindered by the absence of: integrated detector technology, standard commercial components, on-chip sample preparation, standard manufacturing technology and end-to-end system integration. A field-programmable lab-on-a-chip (FPLOC) system based on microelectrode dot array (MEDA) architecture is presented in this research. The MEDA architecture proposes a standard EWOD microfluidic component called ‘microelectrode cell’, which can be dynamically configured into microfluidic components to perform microfluidic operations of the biochip. A proof-of-concept prototype FPLOC, containing a 30 × 30 MEDA, was developed by using generic integrated circuits computer aided design tools, and it was manufactured with standard low-voltage complementary metal-oxide-semiconductor technology, which allows smooth on-chip integration of microfluidics and microelectronics. By integrating 900 droplet detection circuits into microelectrode cells, the FPLOC has achieved large-scale integration of microfluidics and microelectronics. Compared to the full-custom and bottom-up design methods, the FPLOC provides hierarchical top–down design approach, field-programmability and dynamic manipulations of droplets for advanced microfluidic operations.
Lawsonia inermis-mediated synthesis of silver nanoparticles: activity against human pathogenic fungi and bacteria with special reference to formulation of an antimicrobial nanogel
- Author(s): Arpita Gupta ; Shital R. Bonde ; Swapnil Gaikwad ; Avinash Ingle ; Aniket K. Gade ; Mahendra Rai
- Source: IET Nanobiotechnology, Volume 8, Issue 3, p. 172 –178
- DOI: 10.1049/iet-nbt.2013.0015
- Type: Article
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Lawsonia inermis mediated synthesis of silver nanoparticles (Ag-NPs) and its efficacy against Candida albicans, Microsporum canis, Propioniabacterium acne and Trichophyton mentagrophytes is reported. A two-step mechanism has been proposed for bioreduction and formation of an intermediate complex leading to the synthesis of capped nanoparticles was developed. In addition, antimicrobial gel for M. canis and T. mentagrophytes was also formulated. Ag-NPs were synthesized by challenging the leaft extract of L. inermis with 1 mM AgNO3. The Ag-NPs were characterized by Ultraviolet-Visible (UV–Vis) spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Transmission electron microscopy (TEM), nanoparticle tracking and analysis sytem (NTA) and zeta potential was measured to detect the size of Ag-NPs. The antimicrobial activity of Ag-NPs was evaluated by disc diffusion method against the test organisms. Thus these Ag-NPs may prove as a better candidate drug due to their biogenic nature. Moreover, Ag-NPs may be an answer to the drug-resistant microorganisms.
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