IET Nanobiotechnology
Volume 12, Issue 7, October 2018
Volumes & issues:
Volume 12, Issue 7
October 2018
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- Author(s): Adila Nazli ; Muhammad Waleed Baig ; Muhammad Zia ; Muhammad Ali ; Zabta Khan Shinwari ; Ihsan Ul Haq
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 869 –878
- DOI: 10.1049/iet-nbt.2017.0325
- Type: Article
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Theranostic approach provides us a platform where diagnosis and treatment can be carried out simultaneously. Biosynthesis of theranostic-capable nanoparticles (NPs) can be carried out by phytoconstituents present inside the plants that can act as capping as well as stabilising agents by offering several advantages over chemical and physical methods. This article highlights the theranostic role of NPs with emphasis on potential of plants to produce these NPs through ecofriendly approach that is called ‘Green synthesis’. Biosynthesis, advantages, and disadvantages of plant-based theronostics have been discussed for better understanding. Moreover, this article has highlighted the approaches required to optimise the plant-mediated synthesis of NPs and to avoid the toxicity of these agents. Anticipating all of the challenges, the authors expect biogenic NPs can appear as potential diagnostic and therapeutic agents in near future.
- Author(s): Mehrdad Khatami ; Hajar Q. Alijani ; Iraj Sharifi
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 879 –887
- DOI: 10.1049/iet-nbt.2017.0308
- Type: Article
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Recently, researchers succeeded in designing and manufacturing a new class of nanoparticles (NPs) called hybrid NPs. Among hybrid NPs, bimetallic and core–shell NPs were a revolutionary step in NPs science. A large number of green physiochemical and methods for nanostructures synthesis have been published. Eventually, physiochemical methods are either expensive or require the use of chemical compounds for the synthesis of bimetallic and core–shell nanostructures. The main challenges that scientists are facing are making the process cheaper, facile and eco-friendly efficient synthesis process. Green synthesis (biosynthesis) refers to the use of bio-resources (such as bacteria, fungi, plants or their derivatives) for the synthesis of nanostructures. The popularity of the green synthesis of nanostructures is due to their environmental friendliness and no usage of toxic materials, environmental friendliness for the synthesis or stability of nanostructure. Bimetallic and core–shell NPs have many biomedical applications such as removing heavy metals, parasitology, molecular and microbial sensor, gene carrier, single bacterial detection, oligonucleotide detection and so on. The purpose of this study is to discuss briefly the biosynthesised bimetallic and core–shell NPs, their biomedical applications.
Plant-based metallic nanoparticles as potential theranostics agents: bioinspired tool for imaging and treatment
Biosynthesis of bimetallic and core–shell nanoparticles: their biomedical applications – a review
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- Author(s): Jejenija Osuntokun ; Damian C. Onwudiwe ; Eno E. Ebenso
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 888 –894
- DOI: 10.1049/iet-nbt.2017.0277
- Type: Article
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CaO nanoparticles have been prepared using CaCl2 and aqueous extract of broccoli as a precursor and reducing agent, respectively. Different volumes of the aqueous broccoli extract were utilised to obtain Ca(OH)2 and subsequent calcination gave CaO nanoparticles. The synthesised CaO was confirmed by powder X-ray diffraction (XRD). The morphology was studied using transmittance electron microscopy (TEM), and the surface composition of Ca(OH)2 was explored using Fourier transform infrared spectroscopy. The major functional groups present in the capping material responsible for the reduction of the metal salt and the surface passivation of Ca(OH)2 were identified. The XRD pattern revealed cubic phase for all the CaO nanoparticles, and the crystallite size was estimated using Scherrer's equation showed a variation which is dependent on the volume of the extract used. TEM analysis showed different shapes, while the selected area electron diffraction (SAED) results confirmed the crystallinity of the nanoparticles. Thermogravimetric analysis of Ca(OH)2 showed the decomposition product to be CaO. Sample C3, which has the smallest particle size, was used as a catalyst for the degradation of bromocresol green via photo irradiation with ultraviolet light and the result revealed a degradation efficiency of 60.1%.
- Author(s): Iman Bagherpour ; Seyed Morteza Naghib ; Amir Hossein Yaghtin
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 895 –902
- DOI: 10.1049/iet-nbt.2017.0275
- Type: Article
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Here, nanostructured hardystonite bioceramic (Ca2ZnSi2O7) was synthesised from tetraethyl orthosilicate, zinc nitrate hexahydrate, and calcium nitrate tetrahydrate via sol–gel method, dried at 60–120°C, and finally calcinated at 1300°C. X-ray diffraction (XRD) analysis confirmed the formation of hardystonite bioceramic. Afterwards, electrophoretic method was utilised to coat the hardystonite ceramic on 316L stainless steel (SS). Methanol solution was used as suspension solvent. The best deposition procedure was carried out by electrophoretic device in the voltage of 50 V for 5 min. XRD analysis was employed for phase characterisation and scanning electron microscopy was utilised for microstructural and morphological characterisations of the coatings. Chemical composition of the coating was evaluated by energy-dispersive X-ray spectroscopy. The hardystonite coating improved the corrosion resistance of the substrate, so the corrosion current density in the coated samples was less than the uncoated ones (nine times). In order to assess the bioactivity of the coating, simulated body fluid was used. The main results of the coated sample bioactivity demonstrated that the nanostructured hardystonite coating could amend the in vitro SS bioactivity. Therefore, SS coated with nanostructured hardystonite may be a promising candidate to be applied as bioactive hard tissue implants.
- Author(s): Shrestha Roy Goswami and Mukesh Singh
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 903 –908
- DOI: 10.1049/iet-nbt.2018.0007
- Type: Article
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A successful protocol was developed to aid in the reduction in dandruff-causing fungi, namely Malassezia globasa and Malassezia furfur. Both the species were isolated from volunteers aged between 20 and 22 suffering from dandruff, cultured ex vivo, and tested against the presence of synthesised zinc oxide nanoparticles (ZnNP). Direct microscopy, scanning electron microscopy (SEM), and biochemical assays specific to Malassezia species were conducted to identify dandruff-causing fungal species. Microwave-mediated synthesis of ZnNP was performed and characterised by UV–vis, X-ray diffraction, and SEM. The nanoparticles were tested against both Malassezia species and proved highly effective in inhibiting these fungi, although M. furfur was more susceptible than M. globosa. An optimum amount of 100 ppm was found to be sufficient to work as an antifungal agent. Synergistic effects of ZnNP with commercial shampoos were tested, and the result showed enhanced antifungal effects. To mimic the natural biofilm formed by these species on human skin, the formation of fungal biofilm was allowed on polystyrene coverslips. ZnNP was effective in eradication biofilm. Since zinc is an essential mineral for all living organism and is considered as biocompatible, the synthesised nanomaterials can be used in the formulation of antidandruff shampoos.
- Author(s): Palathedath Suresh Kumar ; Sengan Megarajan ; Gajjala Rajendra Kumar Reddy ; Veerappan Anbazhagan
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 909 –914
- DOI: 10.1049/iet-nbt.2018.0048
- Type: Article
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Herein, the authors reported a carbon dots mediated synthesis of gold nanoparticles (AuNPs) at room temperature. Transmission electron microscopy revealed that the AuNPs are spherical in shape with a size of 10 nm. As-prepared AuNPs was immobilised on carbon paste electrode and subjected to electrochemical sensing of an important neurotransmitter dopamine. Differential pulse voltammetry studies revealed sensitive and selective determination of dopamine in the presence of commonly interfering ascorbic acid and uric acid. The linear detection range was 10–600 μM and the limit of detection was 0.7 ± 0.18 μM. The practical application was demonstrated by measuring dopamine in human blood serum and urine samples. The catalytic activity of AuNPs was evaluated by sodium borohydride mediated reduction of nitroaromatic compounds. The reduction kinetics was found to be pseudo-first-order kinetics. All the tested nitroaromatics reduced to corresponding amines in <10 min.
- Author(s): Xin Jia-Ying ; Li Chun-Yu ; Zhang Shuai ; Wang Yan ; Zhang Wei ; Xia Chun-Gu
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 915 –921
- DOI: 10.1049/iet-nbt.2018.0069
- Type: Article
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Methanobactin (Mb) is a small copper-chelating molecule that functions as an agent for copper acquisition, uptake and copper-containing methane monooxygenase catalysis in methane-oxidising bacteria. The UV–visible spectral and fluorescence spectral suggested that Mb/Cu coordination complex as a monomer (Mb-Cu), dimmer (Mb2-Cu) and tetramer (Mb4-Cu) could be obtained at different ratios of Mb to Cu (II). The kinetics of the oxidation of hydroquinone with hydrogen peroxide catalysed by the different Mb/Cu coordination complex were investigated. The results suggested that Mb2-Cu coordination form has highest catalytic capacity. Further, Mb-modified gold nanoparticles (AuNPs) were obtained by ligand exchange and assembled into two- and three-D nanocluster structure by metal-organic coordination as driving force. It has been found that AuNPs increased the catalytic activity of Mb2-Cu on AuNPs. The more significant catalytic activity was exhibited by the nanocluster assembly with multi-catalytic centres. This may be attributed to the multivalent collaborative characteristics of the catalytic active centres in the nanocluster network assembly. The assembly of Mb-modified AuNPs can act as excellent nanoenzyme models for imitating peroxidase.
- Author(s): Shital Vishnu Sable ; Suvidya Ranade ; Satyawati Joshi
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 922 –926
- DOI: 10.1049/iet-nbt.2017.0304
- Type: Article
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The authors have investigated beneficial effects of 1 mM of silver nanoparticles (AgNPs) on agriculturally important plant Pennisetum glaucum (Bajara). The extracellular AgNPs were synthesised using Bacillus subtilis spizizenni and characterised using ultraviolet–visible absorption spectroscopy, Fourier transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). Optical absorption spectrum showed characteristic peak of AgNPs at 423 nm. FT-IR analysis of AgNPs showed peak at 3435 cm−1, which indicates the presence of N–H group (primary, secondary amines and amides) on the surface of AgNPs. TEM studies indicate that synthesised AgNPs have average size of ∼2 nm. Energy dispersive X-ray spectroscopy showed strong signal of Ag at 3 keV. Treatment of 1 mM AgNPs to the bajara seeds was found to be sufficient for excellent germination of seeds within 3 days. There was also significant increase in radicle and plumule length as compared with control bajara seeds according to statistical analysis by one-way analysis of variance, followed by Tukey's test. The percentage of AgNPs detected in root samples was 0.003% (by inductively coupled plasma atomic emission spectroscopy), which is negligible. There is still need to study the bioavailability and the type of interaction of AgNPs with plants, necessary for application in agriculture.
- Author(s): Muhammad Ejaz ; Naveed Iqbal Raja ; Zia-Ur-Rehman Mashwani ; Muhammad Sheeraz Ahmad ; Mubashir Hussain ; Muhammad Iqbal
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 927 –932
- DOI: 10.1049/iet-nbt.2018.0057
- Type: Article
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This study was organised to check the effect of silver nanoparticles and silver nitrate on rice growth against biotic stress. Silver nanoparticles were synthesised by using plant extract as reducing agent, followed by characterisation through UV Vis spectroscopy, XRD, EDS and SEM. Aspergillus application significantly reduced rice plant fresh mass (0.9%), dry mass (0.21%), root length (2.3%), shoot length (5.2%) and root number (1%) in comparison to control. Similarly, leaf area, leaf fresh mass, dry mass and leaf number were also reduced by 23.1, 0.02, 0.11 and 0.9%, respectively. AgNPs and AgNO3 treatments increased the root length (16.2 & 12.8%), shoot length (21 & 20%), root number (8.1 & 6.8%), plant fresh weight (6.4 & 5%) and plant dry weight (4.6 & 3.5%) in 75mg/l treatment of AgNPs and AgNO3 respectively. Similarly, AgNPs and AgNO3 treatment (75 mg/l concentrations) reflected remarkable increase in leaf area (58.8 & 57.2 %), leaf number (4.3 & 3.7 %), leaf fresh weight (1.7 & 1.4 %) and leaf dry weight (0.9 & 0.8 %). Overall AgNPs showed more significant results as compared to AgNO3. The quantity of aflatoxins ranged from 3.1 to 7.7 μg/kg against tolerable limit (4 µg/kg). Overall AgNPs and AgNO3 treatments showed significant results and it could be considered as a strategy for aflatoxin management in rice plants.
- Author(s): Aayushi Biswas ; Chhangte Vanlalveni ; Partha Pradip Adhikari ; Ralte Lalfakzuala ; Lalthazuala Rokhum
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 933 –938
- DOI: 10.1049/iet-nbt.2018.0050
- Type: Article
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Green synthesis of nanoparticles is considered an efficient method when compared with chemical and physical methods because of its bulk production, eco-friendliness and low cost norms. The present study reports, for the first time, green synthesis of silver nanoparticles (AgNPs) at room temperature using Solanum viarum fruit extract. The visual appearance of brownish colour with an absorption band at 450 nm, as detected by ultraviolet-visible spectrophotometer analysis, confirmed the formation of AgNPs. X-ray diffraction confirmed the AgNPs to be crystalline with a face-centred lattice. The transmission electron microscopy-energy dispersive X-ray spectroscopy image showed the AgNPs are poly-dispersed and are mostly spherical and oval in shape with particle size ranging from 2 to 40 nm. Furthermore, Fourier transform-infrared spectra of the synthesised AgNPs confirmed the presence of phytoconstituents as a capping agent. The antimicrobial activity study showed that the AgNPs exhibited high microbial activity against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus susp. aureus, Aspergillus niger, and Candida albicans. The highest antimicrobial activity of AgNPs synthesised by S. viarum fruit extract was observed in P. aeruginosa, S. aureus susp. aureus and C. albicans with zone of inhibition, 26.67 mm.
- Author(s): Maryam Bordbar and Nafiseh Mortazavimanesh
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 939 –945
- DOI: 10.1049/iet-nbt.2017.0266
- Type: Article
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Silver nanoparticles (NPs) are immobilised on pistachio shell surface by Cichorium intybus L. leaves extract as an antioxidant media. The Fourier transform infrared spectra, X-ray diffraction, field-emission scanning electron microscopy equipped with energy-dispersive X-ray spectroscopy, and transmission electron microscope analyses confirmed the support of silver NPs on the pistachio shell (Ag NPs/pistachio shell). Ag NPs on the pistachio shell had a diameter basically in the 10–15 nm range. Reduction reactions of 4-nitrophenol (4-NP), and organic dyes at ambient condition were used in the investigation of the catalytic performance of the prepared catalyst. Through this research, the Ag NPs/pistachio shell shows a high activity and recyclability, and reusability without loss of its catalytic activity.
- Author(s): Zhuanzhuan Zhang and Xianjun Dai
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 946 –950
- DOI: 10.1049/iet-nbt.2017.0260
- Type: Article
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The conditions were optimised for preparing Alginate oligosaccharide (AOS) nanoliposomes, and Caco-2 cell experiments were carried out to examine their antitumour effects. The optimal formulation of AOS nanoliposomes was as follows: a phosphatidylcholine-to-cholesterol ratio of 5.12, AOS concentration of 8.44 mg/mL, Tween 80 concentration of 1.11%, and organic phase to aqueous phase ratio of 5.25. Under the above conditions, the experimental encapsulation efficiency was 65.84%, and the AOS nanoliposomes exhibited a small particle size of 323 nm. After Caco-2 cells were treated with AOS liposomes and AOS for 24 h, AOS nanoliposomes inhibited the growth of Caco-2 cells to a greater extent than AOS at concentrations of 0.0625, 0.125, 0.25, 0.5 and 1 mg/mL (P < 0.01). LDH leakage exhibited a concentration-dependent increase following treatment with 0.5-1 mg/mL AOS nanoliposomes, and the inhibitory effect of AOS nanoliposomes exhibited a more significant difference than AOS (P < 0.01). Cells treated with 0.5 mg/mL and 1 mg/mL AOS nanoliposomes displayed a substantial and significant increase in activity compared with AOS (P < 0.01). Based on these results, AOS nanoliposomes exerted a more significant effect on inhibiting Caco-2 cell proliferation than AOS.
- Author(s): Juliet Sackey ; Serge Berthier ; Malik Maaza ; Thomas Beuvier ; Alain Gibaud
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 951 –955
- DOI: 10.1049/iet-nbt.2017.0320
- Type: Article
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A characteristic feature of the giant owl butterfly, i.e. Caligo memnon, is its big wing eyespot. This feature could serve as deceiving functionality for the butterfly against predators. As evidenced by scanning electron microscope (SEM) image on black part of eyespot, the scales on wing eyespot contain nanostructured ridges and cross-ribs. Applying direct measurement, statistical method, and Fourier analysis, the authors evidence that these nanostructures display order–disorder in their shape and position. The autocorrelation of SEM image provides average values of characteristic periods of the order–disorder nanostructures together with an estimation of corresponding correlation lengths. Linecuts obtained from the Fourier transform of SEM image were also analysed with the Hosemann function to extract similar information. These analyses indicate that the nanostructured order–disorder may contribute to blackness on wing eyespot. The authors thus conclude that the blackness on wing eyespot of C. memnon could be attributed to contributions from both the nanostructured order–disorder and melanin pigment.
- Author(s): Elias E. Elemike ; Damian C. Onwudiwe ; Olusegun K. Abiola ; Kenneth A. Ibe
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 957 –962
- DOI: 10.1049/iet-nbt.2017.0316
- Type: Article
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In this study, French marigold's leaf and flower were used for the synthesis of silver nanoparticles (SNPs) in order to explore their potentials towards bioreduction of Ag+ to Agᵒ. The as-synthesised SNPs were characterised using UV–Vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction, transmission electron microscopy, and zeta-potential analysis. The results obtained showed that the particles are polydispersed with sizes in the range 15.8–42.8 nm. The bioreduction was believed to be due to the amides, aldehyde functional groups, and essential oils present in the extracts as confirmed by the FTIR analysis. The growth mechanism involved in the reaction was studied which revealed oriented attachment (OA) onwards Ostwald ripening in the case of the flower-mediated synthesis and typical OA in the leaf-mediated synthesis. The studied kinetics of the particle formation showed that the reaction is possibly a pseudo-first-order reaction with some diffusion-controlled mechanism which is driven by high surface area to volume ratio in both the leaf- and flower-mediated synthesis.
- Author(s): Manjunath Jenne ; Manjula Kambham ; N.V.K.V.Prasad Tollamadugu ; Hari Prasad Karanam ; Murali Krishna Tirupati ; Ravindra Reddy Balam ; Syed Shameer ; Muralidhar Yagireddy
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 963 –967
- DOI: 10.1049/iet-nbt.2017.0276
- Type: Article
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Nanobiotechnology is one of the emerging fields and its interventions in agriculture is been attracting the scientific community. Herein, the authors first to report on control of groundnut bruchid (Caryedon serratus O.) using nanoscale zinc oxide (ZnONPs) particles and nanoscale chitosan (CNPs) particles-based Azadirachtin formulations. ZnONPs and CNPs were prepared using sol–gel and ion tropic gelation techniques, respectively. Neem seed kernel extract (NSKE) 5% and Neem oil (3000 and 1000 ppm) were encapsulated using the prepared nanoscale materials and characterised using the techniques such as dynamic light scattering, high-resolution transmission electron microscopy. Spherical-shaped nanoparticles were formed after encapsulation with the required bio-materials (ZnONPs 33.1 nm; CNPs 78.8 nm; neem oil encapsulated (3000 ppm) ZnONPs 182.9 nm; NSKE encapsulated ZnONPs 84.9 nm) and observed that the particles are stable (52.3 mV for ZnONPs, −36.2 mV for CNPs, −43.0 mV for neem oil encapsulated (3000 ppm) ZnONPs and −39.4 mV for NSKE encapsulated ZnONPs). NSKE encapsulated CNPs were able to contain groundnut bruchid up to 180 days with 54.61% weight loss compared to other formulations tested. Thus biomaterial encapsulated nanoscale material formulations are proved to be effective in controlling stored grain pests to reduce huge economic losses.
- Author(s): Sania Naz ; Javeed Akhtar ; Muhammad Fayyaz Chaudhary ; Muhammad Zia
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 968 –972
- DOI: 10.1049/iet-nbt.2017.0314
- Type: Article
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In this work, the authors report a facile low-temperature wet-chemical route to prepare morphology-tailored hierarchical structures (HS) of copper oxide. The preparation of copper oxide collides was carried out using varying concentrations of copper acetate and a reducing agent at a constant temperature of 50°C. The prepared HS of CuO were characterised by powdered X-rays diffraction that indicates phase pure having monoclinic structures. The morphology was further confirmed by field-emission scanning electron microscope. It reveals a difference in shape and size of copper oxide HS by changing the concentration of reactants. In order to evaluate the effect of H2O2 on CuO NPs, the prepared CuO are modified by treatment with H2O2. In general trend, CuOH2O2 collide showed enhanced protein kinase inhibition, antibacterial (maximum zone 16.34 mm against Staphylococcus aureus) and antifungal activities in comparison to unmodified CuO collides. These results reveal that CuO HS exhibit antimicrobial properties and can be used as a potential candidate in pharmaceutical industries.
- Author(s): Pei-Ju Lin ; Ming-Chuen Chuang ; Szu-Chung Chang
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 973 –980
- DOI: 10.1049/iet-nbt.2017.0232
- Type: Article
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Patients with serious gingivitis or periodontal diseases suffer from receding gums. Brushing teeth with a toothbrush may result in bleeding gums and new wounds, which increases the difficulty of removing facultative anaerobes from gum pockets, to decrease the damage inflicted on gums, this study proposed a cleaning device that can generate and emit oxygen microbubbles for eliminating facultative anaerobes in the mouth cavity. In this study, the authors conducted simulations with a denture to investigate the efficacy of using this method to remove facultative anaerobes. In this research for the optimal device design, several variables were manipulated including rotation speeds of the bubble generator, different nozzle diameters, and different numbers of nozzle holes. The results revealed that the device is effective in removing facultative anaerobes; moreover, of all design variables, the number of nozzle holes was the factor having the largest effect on anaerobe removal, as it influenced the flow volume and oxygen content of the discharge: the greater the number of nozzles, the greater the flow volume, oxygen content, and efficacy of anaerobe removal.
- Author(s): Deepanshu Bhatt ; Ena Gupta ; Sanket Kaushik ; Vijay Kumar Srivastava ; Juhi Saxena ; Anupam Jyoti
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 981 –986
- DOI: 10.1049/iet-nbt.2018.0051
- Type: Article
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Multiple drug resistance and treatment of contaminated water has become a serious issue in past years. Silver nanoparticles (AgNPs), being bactericidal, non-toxic, cheap and environment friendly behaviour, have drawn attention to overcome these problems. This study has been designed to synthesise AgNPs from Pseudomonas aeruginosa. AgNPs formation was confirmed by colour change and UV–vis spectroscopy. Furthermore, Fourier transform infrared spectroscopy peaks demonstrated the presence of capped proteins as reducing and stabilising agent. Transmission electron microscopy micrograph revealed spherical shape AgNPs with the size ranging between 10 and 20 nm. Antibacterial activity of AgNPs was evaluated against the most prevalent waterborne pathogens enterohaemorrhagic Escherichia coli and Salmonellae typhimurium. Moreover, the antibacterial activity of AgNPs was tested for the treatment of contaminated water which showed attenuation in bacterial load within 8 h as demonstrated by growth kinetics data. Furthermore, AgNPs did not exhibit haemolytic effects on human red blood cells (RBCs) even at 100 mg L−1 concentration of AgNPs. The results herein suggest that AgNPs synthesised by P. aeruginosa under optimised conditions exhibit microbicidal property against waterborne pathogens and having no toxic effect on human RBCs. These AgNPs could be employed for treatment of contaminated water after process optimisation.
- Author(s): Xiaoli Tong ; Na Guo ; Zhiyan Dang ; Qingguang Ren ; Hao Shen
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 987 –993
- DOI: 10.1049/iet-nbt.2017.0230
- Type: Article
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Nanoparticles (NPs), especially biosynthesised in living plants by absorbing soluble salts and reducing metal ions, are extensively used in various fields. This work aimed at investigating the in vivo biosynthesis of silver NPs (Ag-NPs) in maize and the spatial distribution of the NPs and some important nutrient elements in the plant. The content of silver in plant was examined by inductively coupled plasma-atomic emission spectrometer showing that Ag can be absorbed by plant as soluble salts. The NPs in different parts of maize plant were detected and analysed by transmission electron microscopy, demonstrating the synthesis of NPs and their transport from the root to the shoots. Two-dimensional proton induced X-ray emission of silver, chlorine and several nutrient elements elucidated the possible relationship between synthesis of NPs and several nutrient elements in plant tissues. To their knowledge, this is the first report of possibility of synthesis of Ag-NPs in living plants maize (Zea mays L.). This study presents direct evidence for synthesis of NPs and distribution of related nutrient elements in maize, which has great significance for studying synthetic application of NPs in crop plants.
- Author(s): Shima Edalat Fard ; Farzaneh Tafvizi ; Maryam Bikhof Torbati
- Source: IET Nanobiotechnology, Volume 12, Issue 7, p. 994 –1002
- DOI: 10.1049/iet-nbt.2018.5069
- Type: Article
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The aim of this study was to green synthesised silver nanoparticles (AgNPs) using Centella asiatica leaf extract and investigate the cytotoxic and apoptosis-inducing effects of these nanoparticles in MCF-7 breast cancer cell line. The characteristics and morphology of the green synthesised AgNPs were evaluated using transmission electron microscopy, scanning electron microscopy, UV–visible spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The MTT assay was used to investigate the anti-proliferative activity of biosynthesised nanoparticles in MCF-7 cells. Apoptosis test was performed using flow cytometry and expression of caspase 3 and 9 genes. The spherical AgNPs with an average size of 19.17 nm were synthesised. The results showed that biosynthesised AgNPs exhibited cytotoxicity, anti-cancer, apoptosis induction, and increased expression of genes encoding for caspases 3 and 9 in MCF-7 cancer cells in a concentration- and time-dependent manner. It seems that green synthesised AgNPs have potential uses for pharmaceutical industries.
Aqueous extract of broccoli mediated synthesis of CaO nanoparticles and its application in the photocatalytic degradation of bromocrescol green
Synthesis and characterisation of nanostructured hardystonite coating on stainless steel for biomedical application
Microwave-mediated synthesis of zinc oxide nanoparticles: a therapeutic approach against Malassezia species
Facile synthesis of gold nanoparticles using carbon dots for electrochemical detection of neurotransmitter, dopamine in human serum and as a chemocatalyst for nitroaromatic reduction
Cu-induced assembly of methanobactin-modified gold nanoparticles and its peroxidase mimic activity
Role of AgNPs in the enhancement of seed germination and its effect on plumule and radicle length of Pennisetum glaucum
Effect of silver nanoparticles and silver nitrate on growth of rice under biotic stress
Green biosynthesis, characterisation and antimicrobial activities of silver nanoparticles using fruit extract of Solanum viarum
Biosynthesis of waste pistachio shell supported silver nanoparticles for the catalytic reduction processes
Preparation of alginate oligosaccharide nanoliposomes and an analysis of their inhibitory effects on Caco-2 cells
Comparative study on nanostructured order–disorder in the wing eyespots of the giant owl butterfly, Caligo memnon
Surface characterisation and reaction kinetics of silver nanoparticles mediated by the leaf and flower extracts of French marigold (Tagetes patula)
The use of slow releasing nanoparticle encapsulated Azadirachtin formulations for the management of Caryedon serratus O. (groundnut bruchid)
Low-temperature synthesis of hierarchical structures of copper oxide and their superior biological activity
Efficacy of using oxygen microbubble device for facultative anaerobe removal
Bio-fabrication of silver nanoparticles by Pseudomonas aeruginosa: optimisation and antibacterial activity against selected waterborne human pathogens
In vivo biosynthesis and spatial distribution of Ag nanoparticles in maize (Zea mays L.)
Silver nanoparticles biosynthesised using Centella asiatica leaf extract: apoptosis induction in MCF-7 breast cancer cell line
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Nanobiotechnology and its applications in drug delivery system: a review
- Author(s): Imran Khan ; Momin Khan ; Muhammad Naveed Umar ; Deog-Hwan Oh
- Type: Article