http://iet.metastore.ingenta.com
1887

Development of a MEMS-based electrochemical aptasensor for norovirus detection

Development of a MEMS-based electrochemical aptasensor for norovirus detection

For access to this article, please select a purchase option:

Buy article PDF
£12.50
(plus tax if applicable)
Buy Knowledge Pack
10 articles for £75.00
(plus taxes if applicable)

IET members benefit from discounts to all IET publications and free access to E&T Magazine. If you are an IET member, log in to your account and the discounts will automatically be applied.

Learn more about IET membership 

Recommend to library

You must fill out fields marked with: *

Librarian details
Name:*
Email:*
Your details
Name:*
Email:*
Department:*
Why are you recommending this title?
Select reason:
 
 
 
 
 
Micro & Nano Letters — Recommend this title to your library

Thank you

Your recommendation has been sent to your librarian.

Noroviruses (NoVs) are a major cause of acute gastroenteritis worldwide and spread through person-to-person transmission and contaminated food or water. A miniaturised microelectromechanical systems (MEMS)-based electrochemical aptasensor, a biosensor using aptamer as the recognition element for simple, sensitive and rapid detection of NoV has been developed. The novelty of this work is integration of MEMS technology with aptamer to develop a miniaturised and portable electrochemical sensor for environmental pathogen monitoring. An MEMS electrochemical aptasensor device was prepared by immobilising a thiolated DNA aptamer on an on-chip gold (Au) working electrode through the affinity between thiol and Au, and formation of a DNA aptamer monolayer was confirmed by means of cyclic voltammetry as well as visual observation of a fluorescent-labelled aptamer. The MEMS electrochemical aptasensor exhibited rapid and clear square wave voltammetry response against different titres of murine norovirus, an experimental model of human NoV. The possibility of developing a handheld aptasensor platform using a portable electrochemical measurement device is also demonstrated. This research forms initial basis for the development of an electrochemical MEMS-aptasensor platform and its application for virus detection.

References

    1. 1)
    2. 2)
    3. 3)
    4. 4)
    5. 5)
      • S. Hwang , B. Alhatlani , A. Arias .
        5. Hwang, S., Alhatlani, B., Arias, A., et al: ‘Murine norovirus: propagation, quantification and genetic manipulation’, Curr. Protocols Microbiol., 2009, 33, (15K.2), pp. 161.
        . Curr. Protocols Microbiol. , 1 - 61
    6. 6)
    7. 7)
http://iet.metastore.ingenta.com/content/journals/10.1049/mnl.2016.0295
Loading

Related content

content/journals/10.1049/mnl.2016.0295
pub_keyword,iet_inspecKeyword,pub_concept
6
6
Loading
This is a required field
Please enter a valid email address