Hexamethylene-1,3-bis(cetyldimethylammonium bromide) protected low-toxicity ZnSe quantum dots as fluorescent probe for proteins

Yaping Zhong; Chun Deng; Yu He; Yili Ge; Gongwu Song

Hexamethylene-1,3-bis(cetyldimethylammonium bromide) protected low-toxicity ZnSe quantum dots as fluorescent probe for proteins

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Author(s): Yaping Zhong ¹ ; Chun Deng ¹ ; Yu He ^{1, 2} ; Yili Ge ^{1, 2} ; Gongwu Song ^{1, 2}
- Affiliations: 1: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, People's Republic of China;
  2: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, People's Republic of China
Source: Volume 10, Issue 5, May 2015, p. 236 – 240
DOI: 10.1049/mnl.2014.0698 , Online ISSN 1750-0443

Received 25/11/2014, Accepted 09/02/2015, Revised 29/12/2014, Published 13/04/2015

Hexamethylene-1,3-bis(cetyldimethylammonium bromide) (G_16-6-16) protected low-toxicity ZnSe quantum dots (QDs) were successfully synthesised and used as fluorescence probe to detect proteins such as bovine serum albumin (BSA). This method is simple, fast, selective and sensitive. Surfactant G_16-6-16 can interact with proteins through hydrophobic effect, thus leading to the fluorescence quenching of G_16-6-16 protected ZnSe QDs nanometre material. The fluorescence intensity of G_16-6-16 protected ZnSe QDs was linearly proportional to BSA over a concentration range from 0 to 80 nM with a correlation coefficient of 0.993. The detection limit was 0.04 nM. This method was successfully applied to determine BSA in urine samples which shows the good application value of this nanometre material.

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Hexamethylene-1,3-bis(cetyldimethylammonium bromide) protected low-toxicity ZnSe quantum dots as fluorescent probe for proteins

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