Investigation of penetration using atomic force microscope: potential biomarkers of cell membrane

Runhuai Yang; Lianqing Liu; Changlin Zhang; Ning Xi; Jie Yang

Investigation of penetration using atomic force microscope: potential biomarkers of cell membrane

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Author(s): Runhuai Yang ^{1, 2, 3} ; Lianqing Liu ² ; Changlin Zhang ² ; Ning Xi ² ; Jie Yang ¹
- Affiliations: 1: Department of Precision Machinery & Precision Instrumentation, University of Science and Technology of China, Hefei 230000, People's Republic of China;
  2: Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110000, People's Republic of China;
  3: Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon 999077, Hong Kong
Source: Volume 10, Issue 5, May 2015, p. 248 – 252
DOI: 10.1049/mnl.2014.0693 , Online ISSN 1750-0443

Received 12/08/2014, Accepted 04/03/2015, Revised 28/12/2014, Published 13/04/2015

The penetration of cell membrane using atomic force microscope (AFM) is studied. The penetration is monitored by AFM, and the penetration force is measured for three types of cells. The dissipated energy of the cell membrane before and after drug treatment is also calculated and analysed. Experimental results indicate that AFM is an efficient tool for measuring and monitoring the penetration of the cell membrane. Results also show that the penetration force differs significantly from each type of cells, indicating that the penetration force is a potential biomarker of cell membrane to distinguish different types of cells. Moreover, the dissipated energy of cell membrane is studied to be a biomarker to indicate the condition of the cell membrane. After the cell membrane is treated with dimethyl sulfoxide, the dissipated energy of cell membrane decreases.

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Investigation of penetration using atomic force microscope: potential biomarkers of cell membrane

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