A novel hydrogen peroxide (H₂O₂) biosensor was constructed based on Fe₃O₄ magnetic nanoparticles (MNPs) and horseradish peroxidase with graphene–chitosan composite material as a matrix. Fe₃O₄ MNPs were synthesised by chemical co-precipitation with sodium citrate as surfactant. Fourier transform infrared spectroscopy and transmission electron microscopy were applied for the characterisation of the obtained Fe₃O₄ MNPs. The conductivity of different composite films was researched by electrochemical impedance spectroscopy and the electrocatalytic properties of the H₂O₂ biosensor were studied by cyclic voltammetry. Under optimal conditions, experimental results indicated that the biosensor could electrocatalyse the reduction of H₂O₂; the reduction peak current had a good linear relationship with the concentration of H₂O₂ from 2.49 × 10⁻⁵ to 1.67 × 10⁻³ mol/l (R = 0.9990). The detection limit was 3.05 × 10⁻⁶ mol/l (S/N = 3). This novel biosensor showed good sensitivity, stability and repeatability for H₂O₂ detection.

References

1. 1)
  - 1. Chen, D.H., Li, M.Q.: ‘Application and production technology of hydrogen peroxide’, Technol. Dev. Chem. Ind., 2011, 40, pp. 32–35.
2. 2)
  - 16. Wei, H., Wang, E.K.: ‘Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection’, Anal. Chem., 2008, 80, pp. 2250–2254 (doi: 10.1021/ac702203f).
3. 3)
  - 11. Zhang, L., Zhou, N.J., Wang, B., Liu, C., Zhu, G.: ‘Fabrication of Fe3O4/PAH/PSS@Pd core–shell microspheres by layer-by-layer assembly and application in catalysis’, J. Colloid Interf. Sci., 2014, 421, pp. 1–5 (doi: 10.1016/j.jcis.2014.01.023).
4. 4)
  - 26. Kang, X.H., Wang, J., Wu, H., Aksay, I.A., Liu, J., Lin, Y.H.: ‘Glucose oxidase–graphene–chitosan modified electrode for direct electrochemistry and glucose sensing’, Biosens. Bioelectron., 2009, 25, pp. 901–905 (doi: 10.1016/j.bios.2009.09.004).
5. 5)
  - 21. Li, M.G., Xu, S.D., Tang, M., Liu, L., Gao, F., Wang, Y.L.: ‘Direct electrochemistry of horseradish peroxidase on graphene-modified electrode for electrocatalytic reduction towards H2O2’, Electrochim. Acta, 2011, 56, pp. 1144–1149 (doi: 10.1016/j.electacta.2010.10.034).
6. 6)
  - 25. Lu, X.B., Hu, J.Q., Yao, X., Wang, Z.P., Li, J.H.: ‘Composite system based on chitosan and room-temperature ionic liquid: direct electrochemistry and electrocatalysis of hemoglobin’, Biomacromolecules, 2006, 7, pp. 975–980 (doi: 10.1021/bm050933t).
7. 7)
  - 4. Chen, Z.B., Wang, P., Li, Y., Wang, X., Zhu, C.Q.: ‘Determination of hydrogen peroxide by fluorescence quenching of the functional nano-CdTe fluorescence probe’, PTCA (Part B: Chem. Anal.), 2009, 45, pp. 413–418.
8. 8)
  - 10. Tan, X.C., Zhang, J.L., Tan, S.W., et al: ‘Amperometric hydrogen peroxide biosensor based on horseradish peroxidase immobilized on Fe3O4/chitosan modified glassy carbon electrode’, Electroanalysis, 2009, 21, pp. 1514–1520 (doi: 10.1002/elan.200804572).
9. 9)
  - 12. Ou, J., Wang, F., Huang, Y.J., et al: ‘Fabrication and cyto-compatibility of Fe3O4/SiO2/grapheme-CdTe QDs/CS nanocom-posites for drug delivery’, Colloid Surface. B, 2014, 117, pp. 466–472 (doi: 10.1016/j.colsurfb.2013.12.003).
10. 10)
  - 2. Li, L., Lu, H.M., Deng, L.: ‘H2O2 electrochemistry biosensor based on graphene and gold nanorods composites’, Chin. J. Anal. Chem., 2013, 41, pp. 719–724.
11. 11)
  - 6. Wang, J.P., Wang, Z.H., Zhao, D.Y., Xu, C.X.: ‘Facile fabrication of nanoporous PdFe alloy for nonenzymatic electrochemical sensing of hydrogen peroxide and glucose’, Anal. Chim. Acta, 2014, 832, pp. 34–43 (doi: 10.1016/j.aca.2014.04.062).
12. 12)
  - 28. Pan, Q.X., Wang, H.S., Wang, G.X.: ‘Hydrogen peroxide biosensor based on immobilization of hemin in chitosan/nafion matrix’, Chin. J. Anal. Lab., 2010, 29, pp. 91–94.
13. 13)
  - 18. Zhang, L.H., Zhai, Y.M., Gao, N., Wen, D., Dong, S.J.: ‘Sensing H2O2 with layer-by-layer assembled Fe3O4–PDDA nanocomposite film’, Electrochem. Commun., 2008, 10, pp. 1524–1526 (doi: 10.1016/j.elecom.2008.05.022).
14. 14)
  - 27. Srivastava, S., Awasthi, R., Gajbhiye, N.S., Agarwal, V., Singh, A., Gupta, R.K.: ‘Innovative synthesis of citrate-coated superparamagnetic Fe3O4 nanoparticles and its preliminary applications’, J. Colloid Interface Sci., 2011, 359, pp. 104–111 (doi: 10.1016/j.jcis.2011.03.059).
15. 15)
  - 17. Zhang, Z.X., Zhu, H., Wang, X.L., Yang, X.R.: ‘Sensitive electrochemical sensor for hydrogen peroxide using Fe3O4 magnetic nanoparticles as a mimic for peroxidase’, Microchim. Acta, 2011, 174, pp. 183–189 (doi: 10.1007/s00604-011-0600-9).
16. 16)
  - D. Li , M.B. Müller , S. Gilje , R.B. Kaner , G.G. Wallace . Processable aqueous dispersions of graphene nanosheets. Nature Nanotechnol. , 101 - 105
17. 17)
  - 9. Ma, M.P., Cao, J.M., Lian, G.J.: ‘Determination of H2O2 by modified electrode based on L-Cysteine/horseradish peroxidase/nano-Ag/horseradish peroxidase’, J. Instrum. Anal., 2010, 29, pp. 1165–1168.
18. 18)
  - 13. Ranjithkumar, V., Hazeen, A.N., Thamilselvan, M., Vairam, S.: ‘Magnetic activated carbon-Fe3O4 nanocomposites—synthesis and applications in the removal of acid yellow dye 17 from water’, J. Nanosci. Nanotechnol., 2014, 14, pp. 4949–4959 (doi: 10.1166/jnn.2014.9068).
19. 19)
  - 23. Jin, Y.H., Jia, M.Q., Zhang, M., Wen, Q.Q.: ‘Preparation of stable aqueous dispersion of graphene nanosheets and their electrochemical capacitive properties’, Appl. Surf. Sci., 2013, 264, pp. 787–793 (doi: 10.1016/j.apsusc.2012.10.130).
20. 20)
  - 20. Jin, E., Lu, X.F., Cui, L.L., Chao, D.M., Wang, C.: ‘Fabrication of graphene/Prussian blue composite nanosheets and their electrocatalytic reduction of H2O2’, Electrochim. Acta, 2010, 55, pp. 7230–7234 (doi: 10.1016/j.electacta.2010.07.029).
21. 21)
  - 8. Xu, S.X., Wu, J.S., Zhang, Y., Qi, H.L., Zhang, C.X.: ‘Study on the third-generation H2O2 biosensor incorporating HRP immobilizing by nanoparticle Au and carbon nanotubes’, J. Instrum. Anal., 2008, 27, pp. 1099–1102.
22. 22)
  - 7. Zhang, Y.Q., Yang, W.X., Wang, Y.Z., Jia, J.B., Wang, J.G.: ‘Nonenzymatic hydrogen peroxide sensor based on a glassy carbon electrode modified with electrospun PdO-NiO composite nanofibers’, Microchim. Acta, 2013, 180, pp. 1085–1091 (doi: 10.1007/s00604-013-1033-4).
23. 23)
  - 14. Yuan, K.F., Ni, Y.H., Zhang, L.: ‘Facile hydrothermal synthesis of polyhedral Fe3O4 nanocrystals, influencing factors and application in the electrochemical detection of H2O2’, J. Alloys Compd., 2012, 532, pp. 10–15 (doi: 10.1016/j.jallcom.2012.04.007).
24. 24)
  - 24. Shan, C.S., Yang, H.F., Han, D.X., Zhang, Q.X., Ivaska, A., Niu, L.: ‘Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing’, Biosens. Bioelectron., 2010, 25, pp. 1070–1074 (doi: 10.1016/j.bios.2009.09.024).
25. 25)
  - 3. Pan, Y.J., Xie, H.Q., Tan, X.M., Liu, L., Zheng, C.F.: ‘Modification of the iodometric determination of concentration of hydrogen peroxide solution’, PTCA (Part B: Chem. Anal.), 2003, 39, pp. 404–405.
26. 26)
  - 5. Hu, J.M., Shi, W.P., Lin, S.B.: ‘Study on method for determination of H2O2 in cosmetics by HPLC’, Chin. J. Health Lab. Technol., 2003, 13, pp. 593–596.
27. 27)
  - 19. Zhao, X., Zhang, Q.H., Chen, D.J.: ‘Enhanced mechanical properties of graphene-based poly(vinyl alcohol) composites’, Macromolecules, 2010, 43, pp. 2357–2363 (doi: 10.1021/ma902862u).
28. 28)
  - 15. Gao, L.Z., Zhuang, J., Nie, L., et al: ‘Intrinsic peroxidase-like activity of ferromagnetic nanoparticles’, Nat. Nanotechnol., 2007, 2, pp. 577–583 (doi: 10.1038/nnano.2007.260).

Determination of hydrogen peroxide using a biosensor based on Fe₃O₄ magnetic nanoparticles and horseradish peroxidase with graphene–chitosan composite

References

Related content

Determination of hydrogen peroxide using a biosensor based on Fe3O4 magnetic nanoparticles and horseradish peroxidase with graphene–chitosan composite

References

Related content

Determination of hydrogen peroxide using a biosensor based on Fe₃O₄ magnetic nanoparticles and horseradish peroxidase with graphene–chitosan composite