Determination of hydrogen peroxide using a biosensor based on Fe3O4 magnetic nanoparticles and horseradish peroxidase with graphene–chitosan composite
- Author(s): Jianying Qu 1 ; Ying Dong 1 ; Yong Wang 1 ; Tongfang Lou 1 ; Xueping Du 1
-
-
View affiliations
-
Affiliations:
1:
College of Chemistry and Chemical Engineering, Institute of Environmental and Analytical Sciences, Henan University, Kaifeng, Henan 475004, People's Republic of China
-
Affiliations:
1:
College of Chemistry and Chemical Engineering, Institute of Environmental and Analytical Sciences, Henan University, Kaifeng, Henan 475004, People's Republic of China
- Source:
Volume 9, Issue 9,
September 2014,
p.
572 – 576
DOI: 10.1049/mnl.2014.0352 , Online ISSN 1750-0443
A novel hydrogen peroxide (H2O2) biosensor was constructed based on Fe3O4 magnetic nanoparticles (MNPs) and horseradish peroxidase with graphene–chitosan composite material as a matrix. Fe3O4 MNPs were synthesised by chemical co-precipitation with sodium citrate as surfactant. Fourier transform infrared spectroscopy and transmission electron microscopy were applied for the characterisation of the obtained Fe3O4 MNPs. The conductivity of different composite films was researched by electrochemical impedance spectroscopy and the electrocatalytic properties of the H2O2 biosensor were studied by cyclic voltammetry. Under optimal conditions, experimental results indicated that the biosensor could electrocatalyse the reduction of H2O2; the reduction peak current had a good linear relationship with the concentration of H2O2 from 2.49 × 10−5 to 1.67 × 10−3 mol/l (R = 0.9990). The detection limit was 3.05 × 10−6 mol/l (S/N = 3). This novel biosensor showed good sensitivity, stability and repeatability for H2O2 detection.
Inspec keywords: electrochemical impedance spectroscopy; nanofabrication; sensitivity; graphene; thin films; nanocomposites; voltammetry (chemical analysis); biochemistry; thin film sensors; nanoparticles; surfactants; nanosensors; molecular biophysics; magnetic particles; biosensors; biomagnetism; nanomagnetics; iron compounds; transmission electron microscopy; Fourier transform spectra; catalysis; precipitation (physical chemistry); infrared spectra; reduction (chemical); electrical conductivity
Other keywords: composite hlms; graphene-chitosan composite material; electrocatalytic properties; surfactant; Fe3O4 magnetic nanoparticles; electrochemical impedance spectroscopy; conductivity; hydrogen peroxide determination; H2O2 reduction; Fourier transform infrared spectroscopy; transmission electron microscopy; sensitivity; sodium citrate; chemical coprecipitation; horseradish peroxidase; cyclic voltammetry; biosensor; electrocatalyse; Fe3O4-C
Subjects: Micromechanical and nanomechanical devices and systems; Biosensors; Biomolecular interactions, charge transfer complexes; Electrochemical analytical methods; Electrochemistry and electrophoresis; Physical chemistry of biomolecular solutions and condensed states; Methods of nanofabrication and processing; Microsensors and nanosensors; Biosensors; Specific chemical reactions; reaction mechanisms; Biomagnetism; Sensing and detecting devices; Heterogeneous catalysis at surfaces and other surface reactions
References
-
-
1)
-
1. Chen, D.H., Li, M.Q.: ‘Application and production technology of hydrogen peroxide’, Technol. Dev. Chem. Ind., 2011, 40, pp. 32–35.
-
-
2)
-
16. Wei, H., Wang, E.K.: ‘Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection’, Anal. Chem., 2008, 80, pp. 2250–2254 (doi: 10.1021/ac702203f).
-
-
3)
-
11. Zhang, L., Zhou, N.J., Wang, B., Liu, C., Zhu, G.: ‘Fabrication of Fe3O4/PAH/PSS@Pd core–shell microspheres by layer-by-layer assembly and application in catalysis’, J. Colloid Interf. Sci., 2014, 421, pp. 1–5 (doi: 10.1016/j.jcis.2014.01.023).
-
-
4)
-
26. Kang, X.H., Wang, J., Wu, H., Aksay, I.A., Liu, J., Lin, Y.H.: ‘Glucose oxidase–graphene–chitosan modified electrode for direct electrochemistry and glucose sensing’, Biosens. Bioelectron., 2009, 25, pp. 901–905 (doi: 10.1016/j.bios.2009.09.004).
-
-
5)
-
21. Li, M.G., Xu, S.D., Tang, M., Liu, L., Gao, F., Wang, Y.L.: ‘Direct electrochemistry of horseradish peroxidase on graphene-modified electrode for electrocatalytic reduction towards H2O2’, Electrochim. Acta, 2011, 56, pp. 1144–1149 (doi: 10.1016/j.electacta.2010.10.034).
-
-
6)
-
25. Lu, X.B., Hu, J.Q., Yao, X., Wang, Z.P., Li, J.H.: ‘Composite system based on chitosan and room-temperature ionic liquid: direct electrochemistry and electrocatalysis of hemoglobin’, Biomacromolecules, 2006, 7, pp. 975–980 (doi: 10.1021/bm050933t).
-
-
7)
-
4. Chen, Z.B., Wang, P., Li, Y., Wang, X., Zhu, C.Q.: ‘Determination of hydrogen peroxide by fluorescence quenching of the functional nano-CdTe fluorescence probe’, PTCA (Part B: Chem. Anal.), 2009, 45, pp. 413–418.
-
-
8)
-
10. Tan, X.C., Zhang, J.L., Tan, S.W., et al: ‘Amperometric hydrogen peroxide biosensor based on horseradish peroxidase immobilized on Fe3O4/chitosan modified glassy carbon electrode’, Electroanalysis, 2009, 21, pp. 1514–1520 (doi: 10.1002/elan.200804572).
-
-
9)
-
12. Ou, J., Wang, F., Huang, Y.J., et al: ‘Fabrication and cyto-compatibility of Fe3O4/SiO2/grapheme-CdTe QDs/CS nanocom-posites for drug delivery’, Colloid Surface. B, 2014, 117, pp. 466–472 (doi: 10.1016/j.colsurfb.2013.12.003).
-
-
10)
-
2. Li, L., Lu, H.M., Deng, L.: ‘H2O2 electrochemistry biosensor based on graphene and gold nanorods composites’, Chin. J. Anal. Chem., 2013, 41, pp. 719–724.
-
-
11)
-
6. Wang, J.P., Wang, Z.H., Zhao, D.Y., Xu, C.X.: ‘Facile fabrication of nanoporous PdFe alloy for nonenzymatic electrochemical sensing of hydrogen peroxide and glucose’, Anal. Chim. Acta, 2014, 832, pp. 34–43 (doi: 10.1016/j.aca.2014.04.062).
-
-
12)
-
28. Pan, Q.X., Wang, H.S., Wang, G.X.: ‘Hydrogen peroxide biosensor based on immobilization of hemin in chitosan/nafion matrix’, Chin. J. Anal. Lab., 2010, 29, pp. 91–94.
-
-
13)
-
18. Zhang, L.H., Zhai, Y.M., Gao, N., Wen, D., Dong, S.J.: ‘Sensing H2O2 with layer-by-layer assembled Fe3O4–PDDA nanocomposite film’, Electrochem. Commun., 2008, 10, pp. 1524–1526 (doi: 10.1016/j.elecom.2008.05.022).
-
-
14)
-
27. Srivastava, S., Awasthi, R., Gajbhiye, N.S., Agarwal, V., Singh, A., Gupta, R.K.: ‘Innovative synthesis of citrate-coated superparamagnetic Fe3O4 nanoparticles and its preliminary applications’, J. Colloid Interface Sci., 2011, 359, pp. 104–111 (doi: 10.1016/j.jcis.2011.03.059).
-
-
15)
-
17. Zhang, Z.X., Zhu, H., Wang, X.L., Yang, X.R.: ‘Sensitive electrochemical sensor for hydrogen peroxide using Fe3O4 magnetic nanoparticles as a mimic for peroxidase’, Microchim. Acta, 2011, 174, pp. 183–189 (doi: 10.1007/s00604-011-0600-9).
-
-
16)
- D. Li , M.B. Müller , S. Gilje , R.B. Kaner , G.G. Wallace . Processable aqueous dispersions of graphene nanosheets. Nature Nanotechnol. , 101 - 105
-
17)
-
9. Ma, M.P., Cao, J.M., Lian, G.J.: ‘Determination of H2O2 by modified electrode based on L-Cysteine/horseradish peroxidase/nano-Ag/horseradish peroxidase’, J. Instrum. Anal., 2010, 29, pp. 1165–1168.
-
-
18)
-
13. Ranjithkumar, V., Hazeen, A.N., Thamilselvan, M., Vairam, S.: ‘Magnetic activated carbon-Fe3O4 nanocomposites—synthesis and applications in the removal of acid yellow dye 17 from water’, J. Nanosci. Nanotechnol., 2014, 14, pp. 4949–4959 (doi: 10.1166/jnn.2014.9068).
-
-
19)
-
23. Jin, Y.H., Jia, M.Q., Zhang, M., Wen, Q.Q.: ‘Preparation of stable aqueous dispersion of graphene nanosheets and their electrochemical capacitive properties’, Appl. Surf. Sci., 2013, 264, pp. 787–793 (doi: 10.1016/j.apsusc.2012.10.130).
-
-
20)
-
20. Jin, E., Lu, X.F., Cui, L.L., Chao, D.M., Wang, C.: ‘Fabrication of graphene/Prussian blue composite nanosheets and their electrocatalytic reduction of H2O2’, Electrochim. Acta, 2010, 55, pp. 7230–7234 (doi: 10.1016/j.electacta.2010.07.029).
-
-
21)
-
8. Xu, S.X., Wu, J.S., Zhang, Y., Qi, H.L., Zhang, C.X.: ‘Study on the third-generation H2O2 biosensor incorporating HRP immobilizing by nanoparticle Au and carbon nanotubes’, J. Instrum. Anal., 2008, 27, pp. 1099–1102.
-
-
22)
-
7. Zhang, Y.Q., Yang, W.X., Wang, Y.Z., Jia, J.B., Wang, J.G.: ‘Nonenzymatic hydrogen peroxide sensor based on a glassy carbon electrode modified with electrospun PdO-NiO composite nanofibers’, Microchim. Acta, 2013, 180, pp. 1085–1091 (doi: 10.1007/s00604-013-1033-4).
-
-
23)
-
14. Yuan, K.F., Ni, Y.H., Zhang, L.: ‘Facile hydrothermal synthesis of polyhedral Fe3O4 nanocrystals, influencing factors and application in the electrochemical detection of H2O2’, J. Alloys Compd., 2012, 532, pp. 10–15 (doi: 10.1016/j.jallcom.2012.04.007).
-
-
24)
-
24. Shan, C.S., Yang, H.F., Han, D.X., Zhang, Q.X., Ivaska, A., Niu, L.: ‘Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing’, Biosens. Bioelectron., 2010, 25, pp. 1070–1074 (doi: 10.1016/j.bios.2009.09.024).
-
-
25)
-
3. Pan, Y.J., Xie, H.Q., Tan, X.M., Liu, L., Zheng, C.F.: ‘Modification of the iodometric determination of concentration of hydrogen peroxide solution’, PTCA (Part B: Chem. Anal.), 2003, 39, pp. 404–405.
-
-
26)
-
5. Hu, J.M., Shi, W.P., Lin, S.B.: ‘Study on method for determination of H2O2 in cosmetics by HPLC’, Chin. J. Health Lab. Technol., 2003, 13, pp. 593–596.
-
-
27)
-
19. Zhao, X., Zhang, Q.H., Chen, D.J.: ‘Enhanced mechanical properties of graphene-based poly(vinyl alcohol) composites’, Macromolecules, 2010, 43, pp. 2357–2363 (doi: 10.1021/ma902862u).
-
-
28)
-
15. Gao, L.Z., Zhuang, J., Nie, L., et al: ‘Intrinsic peroxidase-like activity of ferromagnetic nanoparticles’, Nat. Nanotechnol., 2007, 2, pp. 577–583 (doi: 10.1038/nnano.2007.260).
-
-
1)
Related content
