Numerical simulation of droplet-based microfluidic chip interfacing with laser desorption/ionisation mass spectrometry target substrate
- Author(s): Chia-Wen Tsao 1 ; Ke-Siang Chen 1 ; Feng-Nan Hwang 2
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View affiliations
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Affiliations:
1:
Department of Mechanical Engineering, National Central University, Jhongli 32001, Taiwan;
2: Department of Mathematics, National Central University, Jhongli 32001, Taiwan
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Affiliations:
1:
Department of Mechanical Engineering, National Central University, Jhongli 32001, Taiwan;
- Source:
Volume 10, Issue 4,
April 2015,
p.
192 – 197
DOI: 10.1049/mnl.2014.0300 , Online ISSN 1750-0443
© The Institution of Engineering and Technology
Received
13/06/2014,
Accepted
03/12/2014,
Revised
12/11/2014,
Published
13/02/2015
Droplet-based microfluidic devices have shown great potential in biomedical applications. When proteomics bioanalysis or biomarker identification is required, off-line microfluidic chip interfacing with mass spectrometry (MS) is necessary. This reported work has focused on the use of numerical simulation of microdroplet formation and dripping for a droplet-based microfluidic-chip interfacing with off-line laser desorption/ionisation MS target substrate. Unlike most studies on the microdroplet formation at the liquid–liquid phase, this work has investigated the microdroplet formation at the liquid–gas phase at various injection flow rates and inner microchannel surface wettability to meet microfluidic-chip–MS interfacing requirements. After the microdroplet formation, subsequent microdroplet dripping analysis was performed. The effects of inner microchannel and outer orifice surface wettability are discussed.
Inspec keywords: drops; MALDI mass spectra; wetting; flow simulation; numerical analysis; microchannel flow; bioMEMS; orifices (mechanical); two-phase flow
Other keywords: liquid-gas phase; liquid-liquid phase; microdroplet formation; biomarker identification; proteomics bioanalysis; injection flow rates; outer orifice surface wettability; droplet-based microfluidic chip; microdroplet dripping; inner microchannel surface wettability; laser desorption-ionisation mass spectrometry target substrate; numerical simulation; biomedical applications
Subjects: Fluid interface activity, spreading; Numerical approximation and analysis; Flows in ducts, channels, and conduits; Biological engineering and techniques; Microfluidics and nanofluidics; Design and modelling of MEMS and NEMS devices; Multiphase flows; Micromechanical and nanomechanical devices and systems; Applied fluid mechanics; Biomedical engineering; General fluid dynamics theory, simulation and other computational methods
References
-
-
1)
-
22. Hufnagel, H., Huebner, A., Gulch, C., Guse, K., Abell, C., Hollfelder, F.: ‘An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets’, Lab Chip, 2009, 9, pp. 1576–1582 (doi: 10.1039/b821695a).
-
-
2)
-
24. Pan, J., Stephenson, A.L., Kazamia, E., et al: ‘Quantitative tracking of the growth of individual algal cells in microdroplet compartments’, Integr. Biol. (Camb.), 2011, 3, pp. 1043–1051 (doi: 10.1039/c1ib00033k).
-
-
3)
- M.G. Pollack , A.D. Shenderov , R.B. Fair . Electrowetting-based actuation of droplets for integrated microfluidics. Lab Chip , 96 - 101
-
4)
-
35. Küster, S.K., Fagerer, S.R., Verboket, P.E., et al: ‘Interfacing droplet microfluidics with matrix-assisted laser desorption/ionization mass spectrometry: label-free content analysis of single droplets’, Anal. Chem., 2013, 85, pp. 1285–1289 (doi: 10.1021/ac3033189).
-
-
5)
-
6. Casadevall i Solvas, X., deMello, A.: ‘Droplet microfluidics: recent developments and future applications’, Chem. Commun., 2011, 47, p. 1936 (doi: 10.1039/c0cc02474k).
-
-
6)
-
17. Ahn, B., Lee, K., Lee, H., et al: ‘Guiding, distribution, and storage of trains of shape-dependent droplets’, Lab Chip, 2011, 11, pp. 3915–3918 (doi: 10.1039/c1lc20729f).
-
-
7)
-
26. Zhang, B.L., Foret, F., Karger, B.L.: ‘High-throughput microfabricated CE/ESI-MS: automated sampling from a microwell plate’, Anal. Chem., 2001, 73, pp. 2675–2681 (doi: 10.1021/ac001432v).
-
-
8)
-
19. Boukellal, H., Selimovic, S., Jia, Y., Cristobal, G., Fraden, S.: ‘Simple, robust storage of drops and fluids in a microfluidic device’, Lab Chip, 2009, 9, pp. 331–338 (doi: 10.1039/b808579j).
-
-
9)
-
12. Chang, Y.H., Lee, G.B., Huang, F.C., Chen, Y.Y., Lin, J.L.: ‘Integrated polymerase chain reaction chips utilizing digital microfluidics’, Biomed. Microdevices, 2006, 8, pp. 215–225 (doi: 10.1007/s10544-006-8171-y).
-
-
10)
-
9. Cho, S.K., Moon, H.J., Kim, C.J.: ‘Creating, transporting, cutting, and merging liquid droplets by electrowetting-based actuation for digital microfluidic circuits’, J. Microelectromech. Syst., 2003, 12, pp. 70–80 (doi: 10.1109/JMEMS.2002.807467).
-
-
11)
-
29. Musyimi, H.K., Guy, J., Narcisse, D.A., Soper, S.A., Murray, K.K.: ‘Direct coupling of polymer-based microchip electrophoresis to online MALDI-MS using a rotating ball inlet’, Electrophoresis, 2005, 26, pp. 4703–4710 (doi: 10.1002/elps.200500317).
-
-
12)
-
10. Tsao, C.W., Kumar, P., Liu, J.K., Devoe, L.: ‘Dynamic electrowetting on nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry’, Anal. Chem., 2008, 80, pp. 2973–2981 (doi: 10.1021/ac7026029).
-
-
13)
-
31. Tsao, C.W., Tao, S., Chen, C.F., Liu, J.K., DeVoe, D.L.: ‘Interfacing microfluidics to LDI-MS by automatic robotic spotting’, Microfluidics Nanofluidics, 2010, 8, pp. 777–787 (doi: 10.1007/s10404-009-0510-x).
-
-
14)
- T. Thorsen , S.J. Maerkl , S.R. Quake . Microfluidic large-scale integration. Science (New York, NY) , 5593 , 580 - 584
-
15)
-
2. Pollack, M.G., Fair, R.B., Shenderov, A.D.: ‘Electro wetting-based actuation of liquid droplets for micro fluidic applications’, Appl. Phys. Lett., 2000, 77, (11), pp. 1725–1726 (doi: 10.1063/1.1308534).
-
-
16)
-
1. Schneider, T., Kreutz, J., Chiu, D.T.: ‘The potential impact of droplet microfluidics in biology’, Anal. Chem., 2013, 85, pp. 3476–3482 (doi: 10.1021/ac400257c).
-
-
17)
-
3. Teh, S.Y., Lin, R., Hung, L.H., Lee, A.P.: ‘Droplet microfluidics’, Lab Chip, 2008, 8, pp. 198–220 (doi: 10.1039/b715524g).
-
-
18)
-
4. Huebner, A., Sharma, S., Srisa-Art, M., Hollfelder, F., Edel, J.B., Demello, A.J.: ‘Microdroplets: a sea of applications?’, Lab Chip, 2008, 8, pp. 1244–1254 (doi: 10.1039/b806405a).
-
-
19)
-
15. Paik, P.Y., Pamula, V.K., Fair, R.B.: ‘Rapid droplet mixers for digital microfluidic systems’, Lab Chip, 2003, 3, pp. 253–259 (doi: 10.1039/b307628h).
-
-
20)
-
20. Schaerli, Y., Hollfelder, F.: ‘The potential of microfluidic water-in-oil droplets in experimental biology’, Mol. Biosyst., 2009, 5, pp. 1392–1404 (doi: 10.1039/b907578j).
-
-
21)
-
27. Wang, Y.X., Cooper, J.W., Lee, C.S., DeVoe, D.L.: ‘Efficient electrospray ionization from polymer microchannels using integrated hydrophobic membranes’, Lab Chip, 2004, 4, pp. 363–367 (doi: 10.1039/b402825b).
-
-
22)
-
21. Trivedi, V., Doshi, A., Kurup, G.K., Ereifej, E., Vandevord, P.J., Basu, A.S.: ‘A modular approach for the generation, storage, mixing, and detection of droplet libraries for high throughput screening’, Lab Chip, 2010, 10, pp. 2433–2442 (doi: 10.1039/c004768f).
-
-
23)
-
6. Nelson, W.C., Kim, C.J.: ‘Droplet actuation by electrowetting-on-dielectric (EWOD): a review’, J. Adhesion Sci. Technol., 2012, 26, pp. 1747–1771.
-
-
24)
-
2. Zhu, Y., Fang, Q.: ‘Analytical detection techniques for droplet microfluidics – a review’, Anal. Chim. Acta, 2013, 787, pp. 24–35 (doi: 10.1016/j.aca.2013.04.064).
-
-
25)
-
32. Gasilova, N., Yu, Q., Qiao, L., Girault, H.H.: ‘On-chip spyhole mass spectrometry for droplet-based microfluidics’, Angew. Chem. Int. Ed., 2014, 53, pp. 4408–4412 (doi: 10.1002/anie.201310795).
-
-
26)
-
23. Tumarkin, E., Tzadu, L., Csaszar, E., et al: ‘High-throughput combinatorial cell co-culture using microfluidics’, Integr. Biol. (Camb.), 2011, 3, pp. 653–662 (doi: 10.1039/c1ib00002k).
-
-
27)
-
18. Fradet, E., McDougall, C., Abbyad, P., Dangla, R., McGloin, D., Baroud, C.N.: ‘Combining rails and anchors with laser forcing for selective manipulation within 2D droplet arrays’, Lab Chip, 2011, 11, p. 4228 (doi: 10.1039/c1lc20541b).
-
-
28)
-
16. Yang, C.H., Lin, Y.S., Huang, K.S., et al: ‘Microfluidic emulsification and sorting assisted preparation of monodisperse chitosan microparticles’, Lab Chip, 2009, 9, pp. 145–150 (doi: 10.1039/b807454b).
-
-
29)
-
28. Yin, N.F., Killeen, K., Brennen, R., Sobek, D., Werlich, M., van de Goor, T.V.: ‘Microfluidic chip for peptide analysis with an integrated HPLC column, sample enrichment column, and nanoelectrospray tip’, Anal. Chem., 2005, 77, pp. 527–533 (doi: 10.1021/ac049068d).
-
-
30)
-
25. Xue, Q.F., Foret, F., Dunayevskiy, Y.M., Zavracky, P.M., McGruer, N.E., Karger, B.L.: ‘Multichannel microchip electrospray mass spectrometry’, Anal. Chem., 1997, 69, pp. 426–430 (doi: 10.1021/ac9607119).
-
-
31)
-
30. Brivio, M., Tas, N.R., Goedbloed, M.H., et al: ‘A MALDI-chip integrated system with a monitoring window’, Lab Chip, 2005, 5, pp. 378–381 (doi: 10.1039/b418986h).
-
-
32)
-
14. Gu, H., Duits, M.H., Mugele, F.: ‘Droplets formation and merging in two-phase flow microfluidics’, Int. J. Mol. Sci., 2011, 12, pp. 2572–2597 (doi: 10.3390/ijms12042572).
-
-
33)
-
34. Wei, J., Buriak, J.M., Siuzdak, G.: ‘Desorption-ionization mass spectrometry on porous silicon’, Nature, 1999, 399, pp. 243–246 (doi: 10.1038/20400).
-
-
34)
-
15. Niu, X., Gulati, S., Edel, J.B., deMello, A.J.: ‘Pillar-induced droplet merging in microfluidic circuits’, Lab Chip, 2008, 8, pp. 1837–1841 (doi: 10.1039/b813325e).
-
-
35)
-
33. Su, Y., Zhu, Y., Fang, Q.: ‘A multifunctional microfluidic droplet-array chip for analysis by electrospray ionization mass spectrometry’, Lab Chip, 2013, 13, pp. 1876–1882 (doi: 10.1039/c3lc00063j).
-
-
1)
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