access icon openaccess Fluorescence imaging under background light with a self-reset complementary metal–oxide–semiconductor image sensor

The authors propose and demonstrate the fluorescence imaging of green fluorescence protein (GFP) expressed in a brain slice with a self-reset complementary metal–oxide–semiconductor image sensor under background light. By using a self-reset function to avoid pixel saturation, the weak fluorescence of GFP was successfully observed, even under background light. The result suggests that the sensor can be applied to in vivo imaging of laboratory animals during light–dark cycles, so that they can observe the different responses to bright and dark environments.

Inspec keywords: proteins; CMOS image sensors; biological techniques; fluorescence

Other keywords: fluorescence imaging; background light; green fluorescence protein; self reset complementary metal–oxide–semiconductor image sensor; brain slice; pixel saturation

Subjects: Biological engineering and techniques; Biophysical instrumentation and techniques; Image sensors; Molecular biophysics

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http://iet.metastore.ingenta.com/content/journals/10.1049/joe.2015.0046
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