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Contribution of ATP synthase to stimulation of respiration by Ca2+ in heart mitochondria

Contribution of ATP synthase to stimulation of respiration by Ca2+ in heart mitochondria

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A variety of experimental conditions were applied with the aim to estimate the correlation between the contribution of ATP synthase to the respiratory flux control and the calcium-induced activation of succinate oxidation in heart mitochondria isolated from rat, rabbit and guinea pig. The sensitivity of respiration in heart mitochondria to the decrease in temperature from 37°C to 28°C decreases in the order rabbit>guinea pig>rat. Ca2+ effect on succinate oxidation rate in state 3 respiration was species- and temperature-dependent and ranged from 0 (rat, 37°C) to +44% (rabbit, 28°C). For mitochondria from all experimental animals, the increase of Ca2+ in physiological range of concentration did not change state 2 respiration rate, and the stimulatory effect of Ca2+ on state 3 respiration was more pronounced at 28°C than at 37°C. The respiratory subsystem was sensitive to Ca2+ ions only in rabbit heart mitochondria. A high positive correlation between Ca2+ ability to stimulate succinate oxidation in state 3 and the control exerted by ATP synthase over the respiratory flux provides argument confirming stimulation of ATP synthase by Ca2+ ions.

Inspec keywords: cardiology; calcium; molecular biophysics; cellular biophysics; enzymes; oxidation; biochemistry

Other keywords: ATP synthase; 37 degC; respiratory flux control; calcium-induced succinate oxidation activation; rat; respiration; heart mitochondria; guinea pig; 28 degC; rabbit; Ca

Subjects: Physics of subcellular structures; Model reactions in molecular biophysics

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