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Dielectrophoretic manipulation of DNA

Dielectrophoretic manipulation of DNA

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The characterisation and spatial manipulation of cells by AC electrokinetic methods such as dielectrophoresis and electrorotation is well established. However, applications to submicroscopical objects like viruses and molecules have been rare. Only recently has the number of such studies risen more quickly due to the availability of suitable electrodes and a growing need for single molecule techniques. Of special interest is the spatial control of single DNA molecules for genetic investigations as well as for the building of well defined structures with nanometre resolution. Here a review is given of dielectrophoretic studies dealing with single and double stranded DNA emphasising single molecule aspects.

Inspec keywords: DNA; microelectrodes; reviews; electrokinetic effects; bioelectric phenomena; genetics; biological techniques; electrophoresis

Other keywords: well defined structures; spatial control; double stranded DNA; electrorotation; electrodes; genetic investigations; submicroscopical objects; nanometre resolution; single DNA molecules; viruses; single stranded DNA; AC electrokinetic methods; single molecule techniques; dielectrophoretic manipulation

Subjects: Bioelectricity; Molecular biophysics; Electrochemistry and electrophoresis; Reviews and tutorial papers; resource letters; Biophysical instrumentation and techniques

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