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access icon free Synthesis, characterisation and anti-tumour activity of biopolymer based platinum nanoparticles and 5-fluorouracil loaded platinum nanoparticles

© The Institution of Engineering and Technology
Received 21/06/2018, Accepted 22/10/2018, Revised 24/09/2018, Published 29/10/2018

A facile and green synthesis of platinum nanoparticles [gum kondagogu platinum nanoparticles (GKPtNP)] using biopolymer- gum kondagogu was developed. The formation of GKPtNP was confirmed by ultraviolet (UV)–visible spectroscopy, scanning electron microscopy–energy dispersive X-ray spectroscopy, transmission electron microscopy, X-ray diffraction, Zeta potential, Fourier transform infrared, inductively coupled plasma mass spectroscopy. The formed GKPtNP are well dispersed, homogeneous with a size of 2–4 ± 0.50 nm, having a negative zeta potential (−46.1 mV) indicating good stability. 5-Fluorouracil (5FU) was loaded onto the synthesised GKPtNP, which leads to the development of a new combination of nanomedicine (5FU–GKPtNP). The in vitro drug release studies of 5FU–GKPtNP in pH 7.4 showed a sustained release profile over a period of 120 min. Agrobacterium tumefaciens induced in vitro potato tumour bioassay was employed for screening the anti-tumour potentials of GKPtNP, 5FU, and 5FU–GKPtNP. The experimental results suggested a complete tumour inhibition by 5FU–GKPtNP at a lower concentration than the GKPtNP and 5FU. Furthermore, the mechanism of anti-tumour activity was assessed by their interactions with DNA using agarose gel electrophoresis and UV-spectroscopic analysis. The electrophoresis results revealed that the 5FU–GKPtNP totally diminishes DNA and the UV-spectroscopic analysis showed a hyperchromic effect with red shift indicating intercalation type of binding with DNA. Over all, the present study revealed that the combined exposure of the nanoformulation resulted in the enhanced anti-tumour effect.

Inspec keywords: Fourier transform infrared spectra; pH; biochemistry; biomedical materials; transmission electron microscopy; nanomedicine; microorganisms; tumours; visible spectra; materials preparation; DNA; electrophoresis; drugs; nanofabrication; molecular biophysics; X-ray chemical analysis; scanning electron microscopy; electrokinetic effects; drug delivery systems; X-ray diffraction; platinum; nanoparticles; polymers; ultraviolet spectra; cancer

Other keywords: in vitro drug release studies; Fourier transform infrared spectroscopy; gum kondagogu platinum nanoparticles; DNA; inductively coupled plasma mass spectroscopy; nanomedicine; X-ray diffraction; time 120.0 min; tumour inhibition; tumour activity; antitumour activity; ultraviolet-visible spectroscopy; zeta potential; UV-spectroscopic analysis; Agrobacterium tumefaciens; in vitro potato tumour bioassay; sustained release profile; UV-visible spectroscopy; Pt; transmission electron microscopy; agarose gel electrophoresis; 5-fluorouracil loaded platinum nanoparticles; 5FU–GKPtNP; biopolymer-based platinum nanoparticles; scanning electron microscopy-energy dispersive X-ray spectroscopy

Subjects: Physical chemistry of biomolecular solutions and condensed states; Electrochemistry and electrophoresis; Preparation of organic materials, polymers and plastics; Other methods of nanofabrication; Patient care and treatment; Biomedical materials; Structure of solid clusters, nanoparticles, nanotubes and nanostructured materials; Nanotechnology applications in biomedicine

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