RT Journal Article
A1 Manish Sharma
A1 Parth Sarthi Nayak
A1 Shreyasi Asthana
A1 Dipankar Mahapatra
A1 Manoranjan Arakha
A1 Suman Jha

PB iet
T1 Biofabrication of silver nanoparticles using bacteria from mangrove swamp
JN IET Nanobiotechnology
VO 12
IS 5
SP 626
OP 632
AB The last decade has observed a rapid advancement in utilising biological system towards bioremediation of metal ions in the form of respective metal nanostructures or microstructures. The process may also be adopted for respective metal nanoparticle biofabrication. Among different biological methods, bacteria-mediated method is gaining great attention for nanoparticle fabrication due to their eco-friendly and cost-effective process. In the present study, silver nanoparticle (AgNP) was synthesised via continuous biofabrication using Aeromonas veronii, isolated from swamp wetland of Sunderban, West Bengal, India. The biofabricated AgNP was further purified to remove non-conjugated biomolecules using size exclusion chromatography, and the purified AgNPs were characterised using UV–visible spectroscopy, X-ray diffraction, field emission scanning electron microscopy and transmission electron microscopy (TEM). Additionally, the presence of proteins as capping and stabilising agents was confirmed by the amide-I and amide-II peaks in the spectra obtained using attenuated total reflection Fourier transform infrared spectroscopy. The size of biofabricated AgNP was 10–20 nm, as observed using TEM. Additionally, biofabricated AgNP shows significant antibacterial potential against E. coli and S. aureus. Hence, biofabricated AgNP using Aeromonas veronii, which found resistant to a significant concentration of Ag ion, showed enhanced antimicrobial activity compared to commercially available AgNP.
K1 bacteria-mediated method
K1 silver nanoparticles
K1 amide-II peaks
K1 amide-I peaks
K1 TEM
K1 metal nanoparticle biofabrication
K1 field emission scanning electron microscopy
K1 nonconjugated biomolecules
K1 size exclusion chromatography
K1 attenuated total reflection Fourier transform infrared spectroscopy
K1 S. aureus
K1 transmission electron microscopy
K1 antibacterial potential
K1 Aeromonas veronii
K1 microstructures
K1 UV-visible spectroscopy
K1 capping agents
K1 X-ray diffraction
K1 stabilising agents
K1 E. coli
K1 purification
K1 metal ions
K1 swamp wetland
K1 biological methods
K1 proteins
K1 eco-friendly
K1 size 10 nm to 20 nm
K1 metal nanostructures
K1 biological system
K1 cost-effective process
K1 antimicrobial activity
K1 mangrove swamp
K1 bioremediation
K1 bacteria
K1 Ag
DO https://doi.org/10.1049/iet-nbt.2017.0205
UL https://digital-library.theiet.org/;jsessionid=mrcd3nux6o6n.x-iet-live-01content/journals/10.1049/iet-nbt.2017.0205
LA English
SN 1751-8741
YR 2018
OL EN