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access icon free Nano-biosensor based on reduced graphene oxide and gold nanoparticles, for detection of phenylketonuria-associated DNA mutation

  • Author(s): Seyed Morteza Seifati 1 ; Navid Nasirizadeh 2 ; Mostafa Azimzadeh 3, 4
    • View affiliations
  • Source: Volume 12, Issue 4, June 2018, p. 417 – 422
    DOI:  10.1049/iet-nbt.2017.0128 , Print ISSN 1751-8741, Online ISSN 1751-875X
© The Institution of Engineering and Technology
Received 29/05/2017, Accepted 05/12/2017, Revised 28/11/2017, Published 07/12/2017

Phenylketonuria (PKU)-associated DNA mutation in newborn children can be harmful to his health and early detection is the best way to inhibit consequences. A novel electrochemical nano-biosensor was developed for PKU detection, based on signal amplification using nanomaterials, e.g. gold nanoparticles (AuNPs) decorated on the reduced graphene oxide sheet on the screen-printed carbon electrode. The fabrication steps were checked by field emission scanning electron microscope imaging as well as cyclic voltammetry analysis. The specific alkanethiol single-stranded DNA probes were attached by self-assembly methodology on the AuNPs surface and Oracet blue was used as an intercalating electrochemical label. The results showed the detection limit of 21.3 fM and the dynamic range of 80–1200 fM. Moreover, the selectivity results represented a great specificity of the nano-biosensor for its specific target DNA oligo versus other non-specific sequences. The real sample simulation was performed successfully with almost no difference than a synthetic buffer solution environment.

Inspec keywords: DNA; biochemistry; field emission scanning electron microscopy; nanoparticles; voltammetry (chemical analysis); electrochemical electrodes; nanosensors; biosensors; gold; nanomedicine; biomedical equipment; self-assembly; molecular biophysics; graphene compounds; electrochemical sensors

Other keywords: screen-printed carbon electrode; cyclic voltammetry; Oracet blue; nanomaterials; reduced graphene oxide sheet; reduced graphene oxide; self-assembly methodology; field emission scanning electron microscopy imaging; intercalating electrochemical label; signal amplification; gold nanoparticles; newborn children; phenylketonuria-associated DNA mutation; electrochemical nanobiosensor; alkanethiol single-stranded DNA probes; Au-CO

Subjects: Patient diagnostic methods and instrumentation; Nanotechnology applications in biomedicine; Chemical sensors; Physical chemistry of biomolecular solutions and condensed states; Biosensors; Electrochemistry and electrophoresis; Microsensors and nanosensors; Biosensors; Chemical sensors; Electrochemical analytical methods; Biomedical measurement and imaging

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